Document Detail


2-D DIGE uterine endothelial proteomic profile for maternal chronic binge-like alcohol exposure.
MedLine Citation:
PMID:  21839868     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Little is known about alcohol effects on the utero-placental compartment during pregnancy. For the first time, we utilized 2-D DIGE quantitative proteomics to evaluate the role of the uterus in Fetal Alcohol Spectrum Disorders (FASD) pathogenesis. Uterine artery endothelial cells were isolated from pregnant ewes, FAC sorted, validated, and maintained in culture. To mimic maternal binge drinking patterns, cells were cultured in the absence or presence of alcohol (300 mg/dl) in a compensating sealed humidified chamber system equilibrated with aqueous alcohol for 3 h on 3 consecutive days for two weeks. CyDye switch combined with 2-D DIGE followed by MALDI-TOF and tandem MS/MS were utilized. Validation was performed using Western immunoblot analysis. Chronic binge-like alcohol significantly (P<0.05) decreased 30 proteins and increased 19 others. Gene-enrichment and functional annotation cluster analysis revealed significant enrichment (P<0.05) in three categories: glutathione S transferase, thioredoxin, and vesicle transport-related. Furthermore, alcohol differentially altered proteins with certain isoforms being downregulated while others were upregulated. In summary, binge alcohol has specific effects on the maternal uterine proteome, especially those related to oxidative stress. The current study also demonstrates a great need to utilize proteomic approaches for diagnostic, mechanistic and therapeutic aspects of FASD.
Authors:
Jayanth Ramadoss; Ronald R Magness
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-08-04
Journal Detail:
Title:  Journal of proteomics     Volume:  74     ISSN:  1876-7737     ISO Abbreviation:  J Proteomics     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-11-14     Completed Date:  2012-03-01     Revised Date:  2014-09-19    
Medline Journal Info:
Nlm Unique ID:  101475056     Medline TA:  J Proteomics     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  2986-94     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier B.V. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Animals
Central Nervous System Depressants / pharmacology*
Endothelium / metabolism*,  pathology
Ethanol / pharmacology*
Female
Fetal Alcohol Spectrum Disorders / etiology,  metabolism*
Pregnancy
Pregnancy Proteins / metabolism*
Proteomics / methods
Sheep
Uterus / blood supply,  metabolism*,  pathology
Grant Support
ID/Acronym/Agency:
AA19446/AA/NIAAA NIH HHS; HD38843/HD/NICHD NIH HHS; HL49210/HL/NHLBI NIH HHS; HL89144/HL/NHLBI NIH HHS; K99 AA019446/AA/NIAAA NIH HHS; K99 AA019446-01/AA/NIAAA NIH HHS; P01 HD038843/HD/NICHD NIH HHS; P01 HD038843-10/HD/NICHD NIH HHS; R01 HL049210/HL/NHLBI NIH HHS; R01 HL049210-13/HL/NHLBI NIH HHS; R01 HL087144/HL/NHLBI NIH HHS; R01 HL087144-04/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Central Nervous System Depressants; 0/Pregnancy Proteins; 3K9958V90M/Ethanol
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