| 2-(4-Chlorophenyl)benzo-1,4-quinone induced ROS-signaling inhibits proliferation in human non-malignant prostate epithelial cells. | |
| | |
MedLine Citation:
|
PMID: 20163859 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Polychlorinated biphenyls (PCBs) and their metabolites are environmental chemical contaminants which can produce reactive oxygen species (ROS) by auto-oxidation of di-hydroxy PCBs as well as the reduction of quinones and redox-cycling. We investigate the hypothesis that 2-(4-chlorophenyl)benzo-1,4-quinone (4-Cl-BQ), a metabolite of 4-chlorobiphenyl (PCB3), induced ROS-signaling inhibits cellular proliferation. Monolayer cultures of exponentially growing asynchronous human non-malignant prostate epithelial cells (RWPE-1) were incubated with 0-6 μM of 4-Cl-BQ and harvested at the end of 72 h of incubation to assess antioxidant enzyme expression, cellular ROS levels, cell growth, and cell cycle phase distributions. 4-Cl-BQ decreased manganese superoxide dismutase (MnSOD) activity, protein, and mRNA levels. 4-Cl-BQ treatment increased dihydroethidium (DHE) fluorescence, which was suppressed in cells pretreated with polyethylene glycol conjugated superoxide dismutase (PEG-SOD). The increase in ROS levels was associated with a decrease in cell growth, and an increase in the percentage of S-phase cells. These effects were suppressed in cells pretreated with PEG-SOD. 4-Cl-BQ treatment did not change the protein levels of phosphorylated H2AX at the end of 72 h of incubation, suggesting that the inhibition in cell growth and accumulation of cells in S-phase at the end of the treatments were probably not due to 4-Cl-BQ induced DNA double strand break. These results demonstrate that MnSOD activity and ROS-signaling perturb proliferation in 4-Cl-BQ treated in vitro cultures of human prostate cells. |
| | |
Authors:
|
Leena Chaudhuri; Ehab H Sarsour; Prabhat C Goswami |
Related Documents
:
|
15964399 - The evaluation of apoptosis and reactive oxygen species in hematopoietic cells derived ... 19144049 - Carbocisteine can scavenge reactive oxygen species in vitro. 9084499 - Generation of free radicals during lipid hydroperoxide-triggered apoptosis in pc12h cells. 21400659 - Cytokine loaded biopolymers as a novel strategy to study stem cells during wound-healin... 19263049 - The role of oxygen in yeast metabolism during high cell density brewery fermentations. 11797039 - Assay of atp in intact cells of the facultative phototroph rhodobacter capsulatus expre... 2842759 - Camp antagonizes interleukin 2-promoted t-cell cycle progression at a discrete point in... 20802299 - The nipple-areola complex epidermis: a prospective systematic study in adult autopsies. 12071459 - Intestinal adaptation in short bowel syndrome. |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-02-16 |
Journal Detail:
|
Title: Environment international Volume: 36 ISSN: 1873-6750 ISO Abbreviation: Environ Int Publication Date: 2010 Nov |
Date Detail:
|
Created Date: 2010-10-04 Completed Date: 2011-01-11 Revised Date: 2011-11-01 |
Medline Journal Info:
|
Nlm Unique ID: 7807270 Medline TA: Environ Int Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 924-30 Citation Subset: IM |
Copyright Information:
|
Copyright © 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
|
Free Radical and Radiation Biology Program, Department of Radiation Oncology, University of Iowa, Iowa City, Iowa, United States. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Biphenyl Compounds
/
toxicity* Cell Proliferation / drug effects* Cells, Cultured Epithelial Cells / drug effects* Humans Male Prostate / drug effects Quinones / toxicity* Reactive Oxygen Species / metabolism* Signal Transduction* Superoxide Dismutase / metabolism |
| Grant Support | |
ID/Acronym/Agency:
|
CA 111365/CA/NCI NIH HHS; P42 ES 013661/ES/NIEHS NIH HHS; P42 ES013661-040002/ES/NIEHS NIH HHS; R01 CA111365-05/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Biphenyl Compounds; 0/Quinones; 0/Reactive Oxygen Species; EC 1.15.1.1/Superoxide Dismutase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Theory underlying a national teacher evaluation program.
Next Document: IntraLase-Enabled Astigmatic Keratotomy for Post-Keratoplasty Astigmatism: On-Axis Vector Analysis.