| {2-[(3-Carboxy-1-oxopropyl) amino]-2-deoxy-D: -Glucose} suppresses proliferation and induces apoptosis in the human esophageal cancer cell line. | |
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MedLine Citation:
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PMID: 20524085 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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The aim of this study was to investigate the molecular mechanisms of apoptosis induced by {2-[(3-Carboxy-1-oxopropyl) amino]-2-deoxy-D: -Glucose} (COPADG) in the esophageal cancer cell line Eca-109 and to establish a relationship between the rate of apoptosis and Fas and Bcl-2 protein expression. Eca-109 cells were cultured under standard condition. Cell growth was measured by MTT assay. Apoptosis and cell proliferation were determined by flow cytometry. Expressions of apoptosis-regulated genes Fas and Bcl-2 were detected by immunohistochemical methods and image analysis. COPADG dose- and time-dependently inhibited the growth of Eca-109 cells in vitro. Incubation of Eca-109 cells with 40 μmol/l of COPADG for 48 h induced significant apoptosis. After drug treatment, Fas protein expression was increased, while Bcl-2 protein expression was decreased. COPADG is able to induce apoptosis in the esophageal cancer cell line Eca-109. The mechanism of apoptosis in these cells may be related to up-regulation of Fas gene expression and the down-regulation of Bcl-2, which may serve as the experimental evidence for development of new drugs for the non-surgical management of human esophageal cancer. |
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Authors:
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Jing Wu; Hong Lu; Xiangchun Ling; Canghai Wang; Ji Rui; Aiqin Wang; Liang Qiao |
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Publication Detail:
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Type: Journal Article Date: 2010-06-04 |
Journal Detail:
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Title: Medical oncology (Northwood, London, England) Volume: 28 ISSN: 1559-131X ISO Abbreviation: Med. Oncol. Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2011-11-18 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9435512 Medline TA: Med Oncol Country: United States |
Other Details:
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Languages: eng Pagination: 986-90 Citation Subset: IM |
Affiliation:
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Department of Gastroenterology and Hepatology, Beijing Shijitan Hospital, Beijing, 100038, China, wu_jing2010@hotmail.com. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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