Document Detail


A 1H STD NMR spectroscopic investigation of sialylnucleoside mimetics as probes of CMP-Kdn synthetase.
MedLine Citation:
PMID:  16897179     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
CMP-Kdn synthetase catalyses the reaction of sialic acids (Sia) and CTP to the corresponding activated sugar nucleotide CMP-Sia and pyrophosphate PP( i ). Saturation Transfer Difference (STD) NMR spectroscopy has been employed to investigate the sub-structural requirements of the enzyme's binding domain. Sialylnucleoside mimetics, where the sialic acid moiety has been replaced by a carboxyl group and a hydrophobic moiety, have been used in NMR experiments, to probe the tolerance of the CMP-Kdn synthetase to such replacements. From our data it would appear that unlike another sialylnucleotide-recognising protein, the CMP-Neu5Ac transport protein, either a phosphate group or other functional groups on the sialic acid framework may play important roles in recognition by the synthetase.
Authors:
Thomas Haselhorst; Melanie Oschlies; Tareq Abu-Izneid; Milton J Kiefel; Joe Tiralongo; Anja K Münster-Kühnel; Rita Gerardy-Schahn; Mark von Itzstein
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Glycoconjugate journal     Volume:  23     ISSN:  0282-0080     ISO Abbreviation:  Glycoconj. J.     Publication Date:  2006 Jul 
Date Detail:
Created Date:  2006-08-09     Completed Date:  2007-10-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8603310     Medline TA:  Glycoconj J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  371-5     Citation Subset:  IM    
Affiliation:
Institute for Glycomics, Griffith University (Gold Coast Campus), PMB 50 Gold Coast Mail Centre, Queensland 9726, Australia.
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MeSH Terms
Descriptor/Qualifier:
Carbohydrate Sequence
Magnetic Resonance Spectroscopy*
Molecular Mimicry*
Molecular Probes / chemistry*
Molecular Sequence Data
N-Acetylneuraminic Acid / chemistry*,  metabolism
Nucleosides / chemistry*,  metabolism
Nucleotidyltransferases / chemistry*
Chemical
Reg. No./Substance:
0/Molecular Probes; 0/Nucleosides; 131-48-6/N-Acetylneuraminic Acid; EC 2.7.7.-/CTP-CMP-3-deoxynonulosonate cytidyltransferase; EC 2.7.7.-/Nucleotidyltransferases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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