Document Detail


1H NMR studies on bovine cyclophilin: preliminary structural characterization of this specific cyclosporin A binding protein.
MedLine Citation:
PMID:  3542017     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
High-field 1H NMR spectroscopy has been used to study the conformation of the cytosolic cyclosporin A binding protein cyclophilin. For the drug-free form of cyclophilin, spectral editing methods in conjunction with a pH titration were used to identify all four His residues present in the protein, and two-dimensional COSY and RELAY spectroscopy was used to elucidate the scalar connectivities in the aromatic and upfield methyl regions of the spectrum. From these scalar connectivities, it was possible to distinguish between inter- and intraresidue dipolar interactions within the aromatic and upfield methyl regions of cyclophilin in the NOESY spectrum. The results of this analysis showed extensive interresidue cross-relaxation among and between these latter spectral regions indicative of the proximal relationships of several of these residues and the presence of a hydrophobic core within cyclophilin.
Authors:
D C Dalgarno; M W Harding; A Lazarides; R E Handschumacher; I M Armitage
Related Documents :
19243167 - Combinatorial synthesis of thrombin-binding aptamers containing iso-guanine.
18989937 - Effect of linker structure on surface density of aptamer monolayers and their correspon...
12185247 - Isolation of high-affinity gtp aptamers from partially structured rna libraries.
20829027 - A novel colorimetric aptasensor using gold nanoparticle for a highly sensitive and spec...
6444947 - Location of a collagen-binding domain in fibronectin.
6809827 - A direct binding assay for mitochondrial autoantibodies.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemistry     Volume:  25     ISSN:  0006-2960     ISO Abbreviation:  Biochemistry     Publication Date:  1986 Nov 
Date Detail:
Created Date:  1987-02-27     Completed Date:  1987-02-27     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370623     Medline TA:  Biochemistry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  6778-84     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Carrier Proteins* / isolation & purification,  metabolism
Cattle
Cyclosporins / metabolism
Magnetic Resonance Spectroscopy / methods
Peptidylprolyl Isomerase
Protein Binding
Protein Conformation
Thymus Gland / metabolism
Grant Support
ID/Acronym/Agency:
AM18778/AM/NIADDK NIH HHS; CA09200/CA/NCI NIH HHS; GM 3224351/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/Cyclosporins; EC 5.2.1.8/Peptidylprolyl Isomerase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Desmoplastic malignant melanoma masquerading as chalazion.
Next Document:  Relationship between cytostatic activity of oxazolopyridocarbazoles and accessibility of DNA interca...