| 1H NMR metabonomics can differentiate the early atherogenic effect of dairy products in hyperlipidemic hamsters. | |
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MedLine Citation:
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PMID: 19324361 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Diet is an important environmental factor modulating the onset of atherosclerosis. The aim of this study was to evaluate the effects of different dairy-based food products on early atherogenesis using both conventional and metabonomic approaches in hyperlipidemic hamsters. The hamsters received up to 200 g/kg of fat as anhydrous butter or cheese made from various milk fats or canola-based oil (CV), in addition to a non-atherogenic low-fat diet. Aortic cholesteryl ester loading was considered to be an early atherogenic point, and metabolic changes linked to atherogenesis were measured using plasma (1)H NMR-based metabonomics. The lowest atherogenicity was obtained with the plant-oil cheese diet, followed by the dairy fat cheese diet, while the greatest atherogenicity was observed with the butter diet (P<0.05). Disease outcome was correlated with conventional plasma biomarkers (total cholesterol, triglycerides, LDL cholesterol, R(2)=0.42-0.60). NMR plasma metabonomics selectively captured part of the diet-induced metabotypes correlated with aortic cholesteryl esters (R(2)=0.63). In these metabotypes, VLDL lipids, cholesterol, and N-acetylglycoproteins (R(2) range: 0.45-0.51) were the most positively correlated metabolites, whereas a multimetabolite response at 3.75 ppm, albumin lysyl residues, and trimethylamine-N-oxide were the most negatively correlated metabolites (R(2) range: 0.43-0.63) of the aortic cholesteryl esters. Collectively, these metabolites predicted 89% of atherogenic variability compared to the 60% predicted by total plasma cholesterol alone. In conclusion, we show that the food environment can modulate the atherogenic effect of dairy fat. This proof-of-principle study demonstrates the first use of plasma metabonomics for improving the prognosis of diet-induced atherogenesis, revealing novel potential disease biomarkers. |
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Authors:
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Jean-Charles Martin; Cécile Canlet; Bernadette Delplanque; Genevieve Agnani; Denis Lairon; Gaëlle Gottardi; Karima Bencharif; Daniel Gripois; Anissa Thaminy; Alain Paris |
Publication Detail:
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Type: Journal Article Date: 2009-02-07 |
Journal Detail:
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Title: Atherosclerosis Volume: 206 ISSN: 1879-1484 ISO Abbreviation: Atherosclerosis Publication Date: 2009 Sep |
Date Detail:
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Created Date: 2009-08-31 Completed Date: 2009-12-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0242543 Medline TA: Atherosclerosis Country: Ireland |
Other Details:
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Languages: eng Pagination: 127-33 Citation Subset: IM |
Affiliation:
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Institut National de la Recherche Agronomique, UMR1260 Nutriments Lipidiques et Prévention des Maladies Métaboliques, Marseille, France. jean-charles.martin@univmed.fr |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Atherosclerosis / etiology*, metabolism Cholesterol Esters / metabolism Cricetinae Dairy Products / adverse effects* Diet, Atherogenic* Diet, Fat-Restricted Dietary Fats / adverse effects Hyperlipidemias / complications Male Mesocricetus Metabolomics Nuclear Magnetic Resonance, Biomolecular |
| Chemical | |
Reg. No./Substance:
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0/Cholesterol Esters; 0/Dietary Fats |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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