Document Detail

1H MRS signals from glutathione may act as predictive markers of apoptosis in irradiated tumour cells.
MedLine Citation:
PMID:  17251247     Owner:  NLM     Status:  MEDLINE    
Inhibition of apoptosis in tumour cells may depend on intracellular reduced glutathione (GSH) level. In this work, GSH levels were studied by (1)H MRS in MCF-7 and HeLa cells, characterised by a different radiosensitivity. Annexin-V test showed that the fraction of apoptotic HeLa cells after irradiation is much higher than in control, although MCF-7 cells did not show a significant apoptosis. MRS signals from GSH (G) show lower intensity in HeLa with respect to MCF-7 cells; the opposite is true for free glutamic acid [glu (g)]. After irradiation, the G/g ratio decreases in MCF-7, although remaining approximately constant in HeLa cells. Buthionine sulfoximine (BSO) treated MCF-7 cells show an increase in the percentage of apoptotic cells; in parallel, G/g ratio behaves as in HeLa. This study indicates that GSH level may act as predictive marker of apoptosis by irradiation.
S Grande; C Giovannini; L Guidoni; A M Luciani; A Palma; A Rosi; O Sapora; V Viti
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Publication Detail:
Type:  Journal Article     Date:  2007-01-24
Journal Detail:
Title:  Radiation protection dosimetry     Volume:  122     ISSN:  0144-8420     ISO Abbreviation:  Radiat Prot Dosimetry     Publication Date:  2006  
Date Detail:
Created Date:  2007-04-30     Completed Date:  2007-07-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8109958     Medline TA:  Radiat Prot Dosimetry     Country:  England    
Other Details:
Languages:  eng     Pagination:  205-6     Citation Subset:  IM    
Department of Technology and Health and INFN Sanità Group, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Roma, Italy.
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MeSH Terms
Apoptosis / radiation effects*
Breast Neoplasms / metabolism*,  pathology*
Cell Line, Tumor
Dose-Response Relationship, Radiation
Glutathione / analysis*
Hela Cells
Magnetic Resonance Spectroscopy / methods*
Protons / diagnostic use*
Radiation Dosage
Reproducibility of Results
Sensitivity and Specificity
Tumor Markers, Biological / analysis*
Reg. No./Substance:
0/Protons; 0/Tumor Markers, Biological; 70-18-8/Glutathione

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