| (1)H MRS detection of glycine residue of reduced glutathione in vivo. | |
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MedLine Citation:
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PMID: 20005139 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Glutathione (GSH) is a powerful antioxidant found inside different kinds of cells, including those of the central nervous system. Detection of GSH in the human brain using (1)H MR spectroscopy is hindered by low concentration and spectral overlap with other metabolites. Previous MRS methods focused mainly on the detection of the cysteine residue (GSH-Cys) via editing schemes. This study focuses on the detection of the glycine residue (GSH-Gly), which is overlapped by glutamate and glutamine (Glx) under physiological pH and temperature. The first goal of the study was to obtain the spectral parameters for characterization of the GSH-Gly signal under physiological conditions. The second goal was to investigate a new method of separating GSH-Gly from Glx in vivo. The characterization of the signal was carried out by utilization of numerical simulations as well as experiments over a wide range of magnetic fields (4.0-14T). The proposed separation scheme utilizes J-difference editing to quantify the Glx contribution to separate it from the GSH-Gly signal. The presented method retains 100% of the GSH-Gly signal. The overall increase in signal to noise ratio of the targeted resonance is calculated to yield a significant SNR improvement compared to previously used methods that target GSH-Cys residue. This allows shorter acquisition times for in vivo human clinical studies. |
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Authors:
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Lana G Kaiser; Małgorzata Marjańska; Gerald B Matson; Isabelle Iltis; Seth D Bush; Brian J Soher; Susanne Mueller; Karl Young |
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Publication Detail:
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Type: Journal Article Date: 2009-11-26 |
Journal Detail:
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Title: Journal of magnetic resonance (San Diego, Calif. : 1997) Volume: 202 ISSN: 1096-0856 ISO Abbreviation: J. Magn. Reson. Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-02-03 Completed Date: 2010-04-23 Revised Date: 2011-07-22 |
Medline Journal Info:
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Nlm Unique ID: 9707935 Medline TA: J Magn Reson Country: United States |
Other Details:
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Languages: eng Pagination: 259-66 Citation Subset: IM |
Copyright Information:
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Copyright 2009 Elsevier Inc. All rights reserved. |
Affiliation:
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Varian Incorporated, Palo Alto, CA, USA. lana.kaiser@varianinc.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Algorithms Computer Simulation Cysteine / chemistry Data Interpretation, Statistical Electromagnetic Fields Glutathione / chemistry* Glycine / chemistry* Humans Nuclear Magnetic Resonance, Biomolecular / methods* Temperature |
| Grant Support | |
ID/Acronym/Agency:
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R01 EB000766-05/EB/NIBIB NIH HHS |
| Chemical | |
Reg. No./Substance:
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52-90-4/Cysteine; 56-40-6/Glycine; 70-18-8/Glutathione |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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