|1H, 15N and 13C backbone resonance assignments of the archetypal serpin α1-antitrypsin.|
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|PMID: 22109101 Owner: NLM Status: MEDLINE|
|Alpha(1)-antitrypsin is a 45-kDa (394-residue) serine protease inhibitor synthesized by hepatocytes, which is released into the circulatory system and protects the lung from the actions of neutrophil elastase via a conformational transition within a dynamic inhibitory mechanism. Relatively common point mutations subvert this transition, causing polymerisation of α(1)-antitrypsin and deficiency of the circulating protein, predisposing carriers to severe lung and liver disease. We have assigned the backbone resonances of α(1)-antitrypsin using multidimensional heteronuclear NMR spectroscopy. These assignments provide the starting point for a detailed solution state characterization of the structural properties of this highly dynamic protein via NMR methods.|
|Mun Peak Nyon; John Kirkpatrick; Lisa D Cabrita; John Christodoulou; Bibek Gooptu|
|Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-11-23|
|Title: Biomolecular NMR assignments Volume: 6 ISSN: 1874-270X ISO Abbreviation: Biomol NMR Assign Publication Date: 2012 Oct|
|Created Date: 2012-09-11 Completed Date: 2013-01-31 Revised Date: 2013-03-08|
Medline Journal Info:
|Nlm Unique ID: 101472371 Medline TA: Biomol NMR Assign Country: Netherlands|
|Languages: eng Pagination: 153-6 Citation Subset: IM|
|Department of Biological Sciences, Institute of Structural and Molecular Biology, Crystallography, Birkbeck College, University of London, Malet Street, London WC1E 7HX, UK.|
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Amino Acid Sequence
Molecular Sequence Data
Nuclear Magnetic Resonance, Biomolecular*
Protein Structure, Secondary
alpha 1-Antitrypsin / chemistry*
|//Biotechnology and Biological Sciences Research Council; //Medical Research Council; //Wellcome Trust|
|0/Carbon Isotopes; 0/Nitrogen Isotopes; 0/Protons; 0/alpha 1-Antitrypsin|
Journal ID (nlm-ta): Biomol NMR Assign
Journal ID (iso-abbrev): Biomol NMR Assign
Publisher: Springer Netherlands, Dordrecht
© The Author(s) 2011
Received Day: 11 Month: 8 Year: 2011
Accepted Day: 2 Month: 11 Year: 2011
Electronic publication date: Day: 23 Month: 11 Year: 2011
pmc-release publication date: Day: 23 Month: 11 Year: 2011
Print publication date: Month: 10 Year: 2012
Volume: 6 Issue: 2
First Page: 153 Last Page: 156
PubMed Id: 22109101
Publisher Id: 9345
|1H, 15N and 13C backbone resonance assignments of the archetypal serpin α1-antitrypsin|
|Mun Peak Nyon1|
|Lisa D. Cabrita12|
Address: +442076792375 email@example.com
Address: +442076316800 firstname.lastname@example.org
1Department of Biological Sciences, Institute of Structural and Molecular Biology, Crystallography, Birkbeck College, University of London, Malet Street, London, WC1E 7HX UK
2Division of Biosciences, Institute of Structural and Molecular Biology, University College London, Gower Street, London, WC1E 6BT UK
Alpha1-antitrypsin is a glycoprotein of the serpin (serine protease inhibitor) protein superfamily (Silverman et al. 2001). It is synthesized within hepatocytes and secreted into the circulatory system, where it is the most abundant human plasma proteinase (Gooptu and Lomas 2009; Travis and Salvesen 1983). The primary function of α1-antitrypsin is the inhibition of neutrophil elastase. Naturally occurring point mutations within the protein such as the Z (Glu342Lys) variant cause it to undergo conformational rearrangement and self-associate into polymer chains (Lomas et al. 1992). The accumulation of α1-antitrypsin polymers within the endoplasmic reticulum (ER) is associated with cellular toxicity and development of liver diseases such as cirrhosis and hepatocellular carcinoma (Eriksson et al. 1986; Perlmutter 2002). Polymerisation abolishes functional activity of α1-antitrypsin and so allows dysregulated elastase activity within the lung, causing emphysema (Elliott et al. 1998). Moreover, extracellular α1-antitrypsin polymers are found within the lung in patients with α1-antitrypsin deficiency related emphysema, and are likely to have pro-inflammatory effects. The combination of loss- and gain-of-function consequences of α1-antitrypsin polymerisation mean there is a need for a strategy that prevents polymerisation and maintains enzyme inhibitory activity (Gooptu et al. 2009). While some small molecules have shown polymerisation blocking effects in vitro, so far they have all abolished the functional activity of α1-antitrypsin (Mahadeva et al. 2002; Mallya et al. 2007). Moreover, there is a lack of structural data defining interactions of the protein with promising lead compounds, preventing their rational modification. Solution NMR is well-suited to provide a residue-specific characterisation of this conformationally heterogeneous protein and to probe its interactions with putative polymerisation blockers. We report here the 1H, 15N and 13C backbone assignments of 45 kDa α1-antitrypsin as a crucial step not only for detailed characterization of this highly dynamic molecule but also towards the application of drug discovery techniques to α1-antitrypsin deficiency.
cDNA coding for 6xHis-tagged α1-antitrypsin within a pQE31 plasmid was transformed into BL21-Gold (DE3) E. coli (Stratagene). Cells were grown in M9 minimal medium (H2O or D2O) at 37°C with 1 g/l of 15NH4Cl (Spectra Stable Isotopes) and 2 g/l of either glucose or 13C-glucose (Sigma-Aldrich) as the sole nitrogen and carbon sources, respectively. Following induction of protein expression at OD600 = 0.6 using isopropyl β-D-1-thiogalactopyranoside (IPTG), cells were incubated at 30°C for 8 h. The cells were collected by centrifugation, resuspended in 10 mM sodium phosphate buffer, pH 8.0, 0.5 M NaCl, 20 mM imidazole, and lysed using a cell disruptor. The supernatant of the cell lysate was loaded onto a HisTrap Crude FF column (5 ml; GE Healthcare) and after washing to baseline with the same buffer, the bound protein was eluted with an imidazole gradient (20 mM – 200 mM). The fractions containing α1-antitrypsin were collected, dialyzed 10 mM Tris, pH 8.0, 1 mM EDTA, and 1 mM β-mercaptoethanol and loaded onto a HiTrap-Q Sepharose column (5 ml; GE Healthcare). Bound protein was then eluted with a 0–0.5 M NaCl gradient. The fractions containing α1-antitrypsin were pooled, dialysed against 25 mM Na2HPO4, 50 mM NaCl and 1 mM EDTA at pH 8.0. For complete back exchange of buried backbone amides in 2H/15N/13C labeled α1-antitrypsin, the protein (1 mg/ml) was unfolded in storage buffer containing 8 M urea for 1 h at room temperature. It was subsequently refolded by dropwise addition to 5 L storage buffer with continuous stirring at 4°C. Precipitated material was removed by centrifugation, and the conformational integrity of the refolded protein was confirmed by non-denaturing and SDS-PAGE and inspection of its 15N TROSY-HSQC spectrum.
NMR spectra were collected from α1-antitrypsin at a typical concentration of 225 μM in 25 mM Na2HPO4, 50 mM NaCl, 1 mM EDTA and 0.001% 4,4-dimethyl-4-silapentane-1-sulfonic acid (DSS) at pH 8.0 or pH 7.0 (10% D2O/90% H2O). Spectra from a sample prior to refolding at pH 8.0 were recorded on a Bruker Avance III 700 MHz spectrometer equipped with an HCN cryoprobe. TROSY (Pershuvin et al. 1997) versions of HNCO, HN(CA)CO, HNCA, HN(CO)CA, HNCACB and HN(CO)CACB spectra (Eletsk et al. 2001; Salzmann et al. 1998, 1999) were acquired with constant-time chemical shift evolution in the 15N dimension and 2H decoupling (1 kHz WALTZ-16) (Shaka et al. 1983) while magnetisation was transverse on CA/CB. Spectra from refolded samples at pH 8.0 and pH 7.0 were acquired on Varian Inova 600, 800 and 900 MHz spectrometers, all equipped with HCN cold-probes. TROSY-HNCA (Yang and Kay 1999) spectra were acquired with 2H decoupling as above and constant-time chemical shift evolution in the 13C indirect dimension. All spectra were processed using NMRPipe (Delaglio et al. 1995) and analysed using the CCPN software suite (Fogh et al. 2002).
The sequential backbone resonance assignments of α1-antitrypsin at pH 8.0 and pH 7.0 have been deposited in the Biological Magnetic Resonance Data Bank (http://www.bmrb.wisc.edu) under the accession number 17804. The 15N-HSQC spectrum of 45 kDa α1-antitrypsin shows excellent cross-peak dispersion (Fig. 1). With 394 residues, α1-antitrypsin is to date one of the largest proteins for which the backbone has been assigned to near completion (Supp Fig. 1). The high molecular weight rendered the backbone assignment a challenging task and this was therefore carried out in successive stages. Initially (stage 1), native 2H/15N/13C-labelled antitrypsin was used, followed by the use of refolded (see Methods) 2H/15N/13C antitrypsin samples at pH 8.0 (stage 2) and pH 7.0 (stage 3). The majority of the secondary-structure elements were assigned in stage 1, except for resonances corresponding to the core β-sheet B and also the loop regions (Supp Fig. 2a), which were assigned during stages 2 (Supp Figs. 2b and 3a) and 3 (Supp Figs. 2c and 3b), respectively. Aside from the additional expected cross-peaks, the 15N-TROSY-HSQC spectra before and after refolding were identical.
82.8 and 79.3% of backbone amide resonances were assigned at pH 8.0 and pH 7.0, respectively. 84.9, 88.3 and 75.1% of the CO, CA and CB resonances, respectively, were assigned at pH 8.0. At pH 7.0, 82.5% of the CA resonances were assigned. The secondary structure of a1-antitrypsin was predicted from the assigned chemical shifts at pH 8.0 using DANGLE (Cheung et al. 2010). The measured CA and CB chemical shifts were first corrected for deuterium isotope shifts (Venters et al. 1996; Gardner et al. 1997). The predicted secondary structure is broadly consistent with that observed crystallographically (Elliott et al. 2000; Fig. 2).
M. P. Nyon, J. Kirkpatrick contributed equally.
We are grateful for the use of the Biological NMR Centre at UCL, the Henry Wellcome Building Biomolecular NMR Facility, University of Birmingham and the MRC Biomedical NMR Centre (Mill Hill). This work was supported by funding from the Wellcome Trust, the Medical Research Council (UK), the Human Frontiers Science Programme, the Biotechnology and Biological Sciences Research Council, Papworth Hospital NHS Trust, and the Birkbeck College Faculty Research Fund.
This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
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Keywords: Keywords Serpin, Antitrypsin, Assignment, Refolding.
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