Document Detail


19F MRI of 3D CEM cells to study the effects of tocopherols and tocotrienols.
MedLine Citation:
PMID:  20439143     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Oximetry of the human T-Lymphoblastoid (CEM) cells was measured using (19)F magnetic resonance imaging ((19)F MRI). The cells were treated with the analogues of vitamin E, alpha-, gamma-, delta-tocopherols and corresponding tocotrienols, ex vivo in three-dimensional (3D) cell culture. The study showed that (19)F MRI allows to measure the effect of the analogues due to changes of oxygenation, which were detected using MRI. Hexafluorobenzene was used as a (19)F MRI probe sensitive to oxygen concentrations. After 72h of treatment in HFBR with alpha-, gamma-, delta-tocopherols the oxygen concentration was 19.9+/-0.8%, 19.3+/-1.4%, 16+/-3.5%, respectively. The oxygen concentration in cells treated with alpha-, gamma-, delta-tocotrienols was found to be 14+/-1.5%, 10+/-1.2% and 8.8+/-1.1%, respectively whereas for the control cells it was 22.1+/-1%. The results show that delta-tocopherol and delta-tocotrienol are the most effective treatments in CEM cells among all the tested analogues.
Authors:
Dorota Bartusik; Boguslaw Tomanek; Danuta Siluk; Roman Kaliszan
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Publication Detail:
Type:  Journal Article     Date:  2010-05-02
Journal Detail:
Title:  Journal of pharmaceutical and biomedical analysis     Volume:  53     ISSN:  1873-264X     ISO Abbreviation:  J Pharm Biomed Anal     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-07-20     Completed Date:  2010-09-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8309336     Medline TA:  J Pharm Biomed Anal     Country:  England    
Other Details:
Languages:  eng     Pagination:  599-602     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2010 Elsevier B.V. All rights reserved.
Affiliation:
National Research Council Canada, Institute for Biodiagnostics West, 3330 Hospital Drive NW, Calgary, Alberta T2N 4N1, Canada. dorota.bartusik@gmail.com
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MeSH Terms
Descriptor/Qualifier:
Cell Line, Tumor
Cell Proliferation / drug effects
Cell Survival / drug effects
Cells, Cultured
Fluorine Radioisotopes / analysis
Humans
Magnetic Resonance Imaging / methods*
T-Lymphocytes / drug effects*
Tocopherols / pharmacology*
Tocotrienols / pharmacology*
Chemical
Reg. No./Substance:
0/Fluorine Radioisotopes; 0/Tocotrienols; 1406-66-2/Tocopherols

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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