| 19F MRI of 3D CEM cells to study the effects of tocopherols and tocotrienols. | |
| | |
MedLine Citation:
|
PMID: 20439143 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Oximetry of the human T-Lymphoblastoid (CEM) cells was measured using (19)F magnetic resonance imaging ((19)F MRI). The cells were treated with the analogues of vitamin E, alpha-, gamma-, delta-tocopherols and corresponding tocotrienols, ex vivo in three-dimensional (3D) cell culture. The study showed that (19)F MRI allows to measure the effect of the analogues due to changes of oxygenation, which were detected using MRI. Hexafluorobenzene was used as a (19)F MRI probe sensitive to oxygen concentrations. After 72h of treatment in HFBR with alpha-, gamma-, delta-tocopherols the oxygen concentration was 19.9+/-0.8%, 19.3+/-1.4%, 16+/-3.5%, respectively. The oxygen concentration in cells treated with alpha-, gamma-, delta-tocotrienols was found to be 14+/-1.5%, 10+/-1.2% and 8.8+/-1.1%, respectively whereas for the control cells it was 22.1+/-1%. The results show that delta-tocopherol and delta-tocotrienol are the most effective treatments in CEM cells among all the tested analogues. |
| | |
Authors:
|
Dorota Bartusik; Boguslaw Tomanek; Danuta Siluk; Roman Kaliszan |
Related Documents
:
|
7738723 - Biochemical basis for the killing of cryptococcus neoformans by rat peritoneal cells. 19440983 - Effect of oxidative stress on the growth of magnetic particles in magnetospirillum magn... 2782393 - Cl-cl exchange in promyelocytic hl-60 cells follows simultaneous rather than ping-pong ... |
Publication Detail:
|
Type: Journal Article Date: 2010-05-02 |
Journal Detail:
|
Title: Journal of pharmaceutical and biomedical analysis Volume: 53 ISSN: 1873-264X ISO Abbreviation: J Pharm Biomed Anal Publication Date: 2010 Nov |
Date Detail:
|
Created Date: 2010-07-20 Completed Date: 2010-09-16 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8309336 Medline TA: J Pharm Biomed Anal Country: England |
Other Details:
|
Languages: eng Pagination: 599-602 Citation Subset: IM |
Copyright Information:
|
Copyright (c) 2010 Elsevier B.V. All rights reserved. |
Affiliation:
|
National Research Council Canada, Institute for Biodiagnostics West, 3330 Hospital Drive NW, Calgary, Alberta T2N 4N1, Canada. dorota.bartusik@gmail.com |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Cell Line, Tumor Cell Proliferation / drug effects Cell Survival / drug effects Cells, Cultured Fluorine Radioisotopes / analysis Humans Magnetic Resonance Imaging / methods* T-Lymphocytes / drug effects* Tocopherols / pharmacology* Tocotrienols / pharmacology* |
| Chemical | |
Reg. No./Substance:
|
0/Fluorine Radioisotopes; 0/Tocotrienols; 1406-66-2/Tocopherols |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The significance of pontomedullar laceration in car occupants following frontal collisions: A retros...
Next Document: Isolation and identification of ester impurities in RG7128, an HCV polymerase inhibitor.