Document Detail

(18)F-FDG Cell Labeling may Underestimate Transplanted Cell Homing: More Accurate, Efficient and Stable Cell Labeling with Hexadecyl-4-[(18)F]fluorobenzoate for In Vivo Tracking of Transplanted Human Progenitor Cells by Positron Emission Tomography.
MedLine Citation:
PMID:  22469629     Owner:  NLM     Status:  Publisher    
Cell therapy is expected to restore perfusion and improve function in the ischemic/infarcted myocardium; however, the biological mechanisms and local effects of transplanted cells remain unclear. To assess cell fate in vivo, hexadecyl-4-[(18)F]fluorobenzoate ((18)F-HFB) cell labeling was evaluated for tracking human circulating progenitor cells (CPCs) with positron emission tomography (PET), and was compared to the commonly used 2-[(18)F] fluoro-2-deoxy-D-glucose ((18)F-FDG) labeling method in a rat myocardial infarction model. CPCs were labeled with (18)F-HFB or (18)F-FDG ex vivo under the same conditions. (18)F-HFB cell labeling efficiency (23.4±7.5%) and stability (4h, 88.4±6.0%) were superior to (18)F-FDG (7.6±4.1% and 26.6±6.1%, respectively; p<0.05). Neither labeling approach significantly altered cell viability, phenotype or migration potential up to 24h post-labeling. Two weeks after left anterior descending coronary artery ligation, rats received echo-guided intramyocardial injection in the infarct border zone with: (18)F-HFB-CPCs; (18)F-FDG-CPCs; (18)F-HFB; or (18)F-FDG. Dynamic PET imaging of both (18)F-HFB-CPCs and (18)F-FDG-CPCs demonstrated that only 16-37% of the initial injection dose (ID) was retained in the injection site at 10min post-delivery, and remaining activity fell significantly over the first 4h post-transplantation. The (18)F-HFB-CPC signal in the target area at 2h (23.7±14.7%ID/g) and 4h (17.6±13.3%ID/g) post-injection was greater than that of (18)F-FDG-CPCs (5.4±2.3%ID/g and 2.6±0.7%ID/g, respectively; p<0.05). Tissue biodistribution confirmed the higher radioactivity in the border zone of (18)F-HFB-CPC rats. Immunostaining of heart tissue sections revealed no significant difference in cell retention between two labeled-cell transplantation groups. Good correlation with biodistribution results was observed in the (18)F-HFB-CPC rats (r=0.81, p<0.05). Compared to (18)F-FDG, labeling human CPCs with (18)F-HFB provides a more efficient, stable, and accurate way to quantify the distribution of transplanted cells. (18)F-HFB cell labeling with PET imaging offers a better modality to enhance our understanding of early retention, homing, and engraftment with cardiac cell therapy.
Yan Zhang; Jean N Dasilva; Tayebeh Hadizad; Stephanie Thorn; Drew Kuraitis; Jennifer M Renaud; Ali Ahmadi; Myra Kordos; Robert A Dekemp; Rob S Beanlands; Erik J Suuronen; Marc Ruel
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-3-28
Journal Detail:
Title:  Cell transplantation     Volume:  -     ISSN:  1555-3892     ISO Abbreviation:  -     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-4-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9208854     Medline TA:  Cell Transplant     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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