Document Detail


18α-glycyrrhetinic acid extracted from Glycyrrhiza radix inhibits proliferation and promotes apoptosis of hepatic stellate cell line.
MedLine Citation:
PMID:  23362936     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
OBJECTIVE: To evaluate the effect of 18α-glycyrrhetinic acid (18α-GA) on the proliferation and apoptosis of hepatic stellate cells (HSCs) and its underlying mechanisms. METHODS: Cell cycle and apoptosis of HSCs were analyzed by flow cytometry, and changes in cell cycle and apoptosis-related proteins were analyzed by western blot. The effect of 18α-GA on nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) DNA binding activity was measured by ArrayStar transcription factor activity assay. Cells were pretreated with or without a selective PPAR-γ antagonist, GW9662, before 18α-GA treatment. RESULTS: 18α-GA markedly reduced LX-2 cell numbers by 14.76% and 31.19% after 48 h and 72 h of treatment, respectively (P < 0.05). 18α-GA also significantly increased the percentage of LX-2 cells in phase G0/G1, and decreased that in phase S after treatment for 48 h and 72 h. 18α-GA increased apoptosis to 6.8% at 48 h, compared with control (2.5%), and at 72 h, the percentages of apoptotic cells in control and the treatment groups were 3.1% and 15.6%, respectively in LX-2 cells (P<0.01). The similar changes occurred in CCL(4) -cirrhotic fat-storing cells (CFSCs). Furthermore, 18α-GA induced expression of peroxisome proliferator-activated receptor-γ (PPAR-γ), and altered some cell cycle and apoptosis-related proteins. 18α-GA also inhibited NF-κB DNA binding activity. All of these effects were abrogated by GW9662. CONCLUSIONS: 18α-GA inhibits the proliferation of activated HSCs and induces apoptosis in culture. It also increases PPAR-γ expression and decreases NF-κB DNA binding activity, which may be involved in these effects.
Authors:
Lei Zong; Ying Qu; Ming Yi Xu; Yu Wei Dong; Lun Gen Lu
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-31
Journal Detail:
Title:  Journal of digestive diseases     Volume:  -     ISSN:  1751-2980     ISO Abbreviation:  J Dig Dis     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-31     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101302699     Medline TA:  J Dig Dis     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2013 The Authors. Journal of Digestive Diseases © 2013 Chinese Medical Association Shanghai Branch, Chinese Society of Gastroenterology, Renji Hospital Affiliated to Shanghai Jiaotong University School of Medicine and Wiley Publishing Asia Pty Ltd.
Affiliation:
Department of Gastroenterology, Shanghai First People's Hospital, Shanghai Jiaotong University, Shanghai; Department of Gastroenterology, Weifang People's Hospital, Weifang, Shandong Province, China.
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