Document Detail

18α-glycyrrhetinic acid extracted from Glycyrrhiza radix inhibits proliferation and promotes apoptosis of hepatic stellate cell line.
MedLine Citation:
PMID:  23362936     Owner:  NLM     Status:  Publisher    
OBJECTIVE: To evaluate the effect of 18α-glycyrrhetinic acid (18α-GA) on the proliferation and apoptosis of hepatic stellate cells (HSCs) and its underlying mechanisms. METHODS: Cell cycle and apoptosis of HSCs were analyzed by flow cytometry, and changes in cell cycle and apoptosis-related proteins were analyzed by western blot. The effect of 18α-GA on nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) DNA binding activity was measured by ArrayStar transcription factor activity assay. Cells were pretreated with or without a selective PPAR-γ antagonist, GW9662, before 18α-GA treatment. RESULTS: 18α-GA markedly reduced LX-2 cell numbers by 14.76% and 31.19% after 48 h and 72 h of treatment, respectively (P < 0.05). 18α-GA also significantly increased the percentage of LX-2 cells in phase G0/G1, and decreased that in phase S after treatment for 48 h and 72 h. 18α-GA increased apoptosis to 6.8% at 48 h, compared with control (2.5%), and at 72 h, the percentages of apoptotic cells in control and the treatment groups were 3.1% and 15.6%, respectively in LX-2 cells (P<0.01). The similar changes occurred in CCL(4) -cirrhotic fat-storing cells (CFSCs). Furthermore, 18α-GA induced expression of peroxisome proliferator-activated receptor-γ (PPAR-γ), and altered some cell cycle and apoptosis-related proteins. 18α-GA also inhibited NF-κB DNA binding activity. All of these effects were abrogated by GW9662. CONCLUSIONS: 18α-GA inhibits the proliferation of activated HSCs and induces apoptosis in culture. It also increases PPAR-γ expression and decreases NF-κB DNA binding activity, which may be involved in these effects.
Lei Zong; Ying Qu; Ming Yi Xu; Yu Wei Dong; Lun Gen Lu
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-31
Journal Detail:
Title:  Journal of digestive diseases     Volume:  -     ISSN:  1751-2980     ISO Abbreviation:  J Dig Dis     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-31     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101302699     Medline TA:  J Dig Dis     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2013 The Authors. Journal of Digestive Diseases © 2013 Chinese Medical Association Shanghai Branch, Chinese Society of Gastroenterology, Renji Hospital Affiliated to Shanghai Jiaotong University School of Medicine and Wiley Publishing Asia Pty Ltd.
Department of Gastroenterology, Shanghai First People's Hospital, Shanghai Jiaotong University, Shanghai; Department of Gastroenterology, Weifang People's Hospital, Weifang, Shandong Province, China.
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