Document Detail


1,8-dihydroxynaphthalene (DHN)-melanin biosynthesis inhibitors increase erythritol production in Torula corallina, and DHN-melanin inhibits erythrose reductase.
MedLine Citation:
PMID:  12788746     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The yeast Torula corallina is a strong erythritol producer that is used in the industrial production of erythritol. However, melanin accumulation during culture represents a serious problem for the purification of erythritol from the fermentation broth. Melanin biosynthesis inhibitors such as 3,4-dihydroxyphenylalanine and 1,8-dihydroxynaphthalene (DHN)-melanin inhibitors were added to the T. corallina cultures. Only the DHN-melanin inhibitors showed an effect on melanin production, which suggests that the melanin formed during the culturing of T. corallina is derived from DHN. This finding was confirmed by the detection of a shunt product of the pentaketide pathway, flaviolin, and elemental analysis. Among the DHN-melanin inhibitors, tricyclazole was the most effective. Supplementation with tricyclazole enhanced the production of erythritol while significantly inhibiting the production of DHN-melanin and DHN-melanin biosynthetic enzymes, such as trihydroxynaphthalene reductase. The erythrose reductase from T. corallina was purified to homogeneity by ion-exchange and affinity chromatography. Purified erythrose reductase was significantly inhibited in vitro in a noncompetitive manner by elevated levels of DHN-melanin. In contrast, the level of erythrose reductase activity was unaffected by increasing concentrations of tricyclazole. These results suggest that supplemental tricyclazole reduces the production of DHN-melanin, which may lead to a reduction in the inhibition of erythrose reductase and a higher yield of erythritol. This is the first report to demonstrate that melanin biosynthesis inhibitors increase the production of a sugar alcohol in T. corallina.
Authors:
Jung-Kul Lee; Hyung-Moo Jung; Sang-Yong Kim
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  69     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2003 Jun 
Date Detail:
Created Date:  2003-06-05     Completed Date:  2003-10-01     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3427-34     Citation Subset:  IM    
Affiliation:
BioNgene Co., Ltd., Chongro-Ku, Seoul 110-521, Korea. jkrhee@biongene.com
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MeSH Terms
Descriptor/Qualifier:
Aldehyde Reductase / antagonists & inhibitors*,  biosynthesis
Cryptococcus / growth & development,  metabolism*
Culture Media
Erythritol / metabolism*
Melanins / antagonists & inhibitors*,  biosynthesis
Naphthols / antagonists & inhibitors*,  metabolism
Thiazoles / pharmacology*
Chemical
Reg. No./Substance:
0/Culture Media; 0/Melanins; 0/Naphthols; 0/Thiazoles; 149-32-6/Erythritol; 569-42-6/1,8-dihydroxynaphthalene; EC 1.1.1.21/Aldehyde Reductase; R7U3MEU7VR/tricyclazole
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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