Document Detail

18 S rRNA degradation is not accompanied by altered rRNA transport at early times following irradiation of HeLa cells.
MedLine Citation:
PMID:  2300670     Owner:  NLM     Status:  MEDLINE    
The effect of ionizing radiation (137Cs) on processing and transport of ribosomal RNA (rRNA) was studied by pulse-labeling HeLa S3 cells with [3H]uridine immediately prior to irradiation. This approach permits kinetic analysis of processing of 45 S rRNA (radiolabeled predominantly prior to irradiation) into its 28 S and 18 S rRNA daughter species following irradiation. By this technique, we have recently demonstrated an increase in the normal 28 S:18 S rRNA stoichiometric ratio of 1:1 to as high as 1.6:1 during the interval 5 to 20 h following irradiation of HeLa cells at greater than or equal to 7.5 Gy. Alterations in 28 S:18 S ratio were evaluated in greater detail at early times following irradiation, up to 2 h. The 28 S:18 S ratio was found to be maximal at 1 h after radiation, at about 2:1, following 5 or 10 Gy. Using a method for rapid separation of nucleus from cytoplasm, transport of rRNA from nucleus to cytoplasm was also evaluated during this period. Despite an increase in the rate of 45 S rRNA processing, as well as an increased 28 S:18 S ratio, no alterations in transport from nucleus to cytoplasm were detected. This lack of transport alteration suggests that accumulation of excess 28 S rRNA is restricted to the nucleus, where it may represent an early step in the process of radiation-induced cell killing.
P Fuchs; J M Krolak; D McClain; K W Minton
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Radiation research     Volume:  121     ISSN:  0033-7587     ISO Abbreviation:  Radiat. Res.     Publication Date:  1990 Jan 
Date Detail:
Created Date:  1990-03-01     Completed Date:  1990-03-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0401245     Medline TA:  Radiat Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  67-70     Citation Subset:  IM; S    
Israel Institute for Biological Research, Ness-Ziona.
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MeSH Terms
Biological Transport
Cell Nucleus / metabolism
Cesium Radioisotopes
Cytoplasm / metabolism
Gamma Rays
Hela Cells / radiation effects
RNA, Ribosomal / metabolism*
RNA, Ribosomal, 18S / metabolism
Time Factors
Reg. No./Substance:
0/Cesium Radioisotopes; 0/RNA, Ribosomal; 0/RNA, Ribosomal, 18S

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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