Document Detail


17beta-oestradiol and progesterone regulate anandamide synthesis in the rat uterus.
MedLine Citation:
PMID:  19192341     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Anandamide is an endocannabinoid known to participate in reproductive processes. This study observed that 17beta-oestradiol and progesterone modulated the production of anandamide and its metabolizing enzymes in the rat uterus. Anandamide production was highest at the oestrous stage and 17beta-oestradiol and progesterone stimulated its synthesis in ovariectomized rats. During early pregnancy, anandamide production remained constant on days 1-5 of gestation and diminished towards day 6. On day 6, implantation sites showed lower synthesis compared with interimplantation sites. In the delayed implantation model, 17beta-oestradiol inhibited anandamide synthesis compared with progesterone. During pseudopregnancy, anandamide production did not decrease towards day 6 as occurred during normal gestation. The administration of 17beta-oestradiol augmented anandamide production in rats on day 5 of pseudopregnancy; the treatment with mifepristone did not produce any change in anandamide synthesis. Anandamide-metabolizing enzymes were regulated by progesterone and 17beta-oestradiol. The effect of ovarian hormones on the synthesis of anandamide depends on different physiological conditions, oestrous cycle and early pregnancy, and on the presence of the activated blastocyst. Thus, ovarian hormones, as signals that emanate from the mother, operate in conjunction with the blastocyst intrinsic programme, regulating the synthesis of anandamide in a specific manner during crucial reproductive events that may compromise pregnancy outcome.
Authors:
María L Ribeiro; Claudia A Vercelli; Micaela S Sordelli; Mariana G Farina; Marcos Cervini; Silvia Billi; Ana M Franchi
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Reproductive biomedicine online     Volume:  18     ISSN:  1472-6491     ISO Abbreviation:  Reprod. Biomed. Online     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-02-04     Completed Date:  2009-05-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101122473     Medline TA:  Reprod Biomed Online     Country:  England    
Other Details:
Languages:  eng     Pagination:  209-18     Citation Subset:  IM    
Affiliation:
Laboratory of Physiopathology of Pregnancy and Labor, Center for Pharmacological and Botanical Studies, School of Medicine (National Research Council, University of Buenos Aires), Paraguay, Buenos Aires, Argentina. marialribeiro@yahoo.com.ar
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MeSH Terms
Descriptor/Qualifier:
Amidohydrolases / genetics,  metabolism
Animals
Arachidonic Acids / biosynthesis*
Embryo Implantation / drug effects,  genetics
Endocannabinoids / biosynthesis
Estradiol / pharmacology*
Estrous Cycle / drug effects,  genetics,  metabolism
Female
Gene Expression Regulation, Enzymologic / drug effects
Ovariectomy
Phospholipase D / genetics,  metabolism
Polyunsaturated Alkamides
Pregnancy
Progesterone / pharmacology*
Pseudopregnancy / genetics,  metabolism
Rats
Rats, Wistar
Time Factors
Uterus / drug effects*,  metabolism
Chemical
Reg. No./Substance:
0/Arachidonic Acids; 0/Endocannabinoids; 0/Polyunsaturated Alkamides; 50-28-2/Estradiol; 57-83-0/Progesterone; 94421-68-8/anandamide; EC 3.1.4.4/NAPE-PLD protein, rat; EC 3.1.4.4/Phospholipase D; EC 3.5.-/Amidohydrolases; EC 3.5.1.-/fatty-acid amide hydrolase

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