Document Detail


17beta-Estradiol affects osmoregulation in Fundulus heteroclitus.
MedLine Citation:
PMID:  15465664     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of 17beta-estradiol (E(2)) on osmoregulatory performance was examined in the euryhaline killifish, Fundulus heteroclitus. Fish were injected once with 1, 2 and 5 microg g(-1) E(2) and, 6 h after injection, transferred from 1 ppt seawater (SW) to full strength SW (40 ppt) or from SW to 1 ppt SW. In another set of experiments, fish were injected four times on alternate days with 2 microg g(-1) E(2) and then, 6 h after the last injection, transferred from 1 ppt SW to SW or from SW to 1 ppt SW. Fish were sampled 18 h after transfer (i.e., 24 h post-injection), and plasma osmolality, Na(+) and Cl(-) concentration and gill K(+)-pNPPase activity (a reflection of the sodium pump) were examined. Transfer from 1 ppt SW to SW resulted in significantly increased plasma osmolality, but did not affect gill K(+)-pNPPase activity. A single dose of E(2) (1, 2 and 5 microg g(-1)) prior to transfer from 1 ppt SW to SW increased plasma osmolality and decreased gill K(+)-pNPPase activity in a dose-dependent manner. Prolonged treatment with E(2) increased plasma osmolality and decreased gill K(+)-pNPPase activity in 1 ppt SW-adapted fish. Transfer of fish thus treated from 1 ppt SW to SW increased plasma osmolality and did not alter gill K(+)-pNPPase activity. Transfer from SW to 1 ppt SW had no significant effect on plasma osmolality or gill K(+)-pNPPase activity. Only the highest single dose of E(2) (5 microg g(-1)) prior to transfer from SW to 1 ppt SW decreased gill K(+)-pNPPase activity. Prolonged treatment with 2 microg g(-1) E(2) decreased gill K(+)-pNPPase activity only following transfer from SW to 1 ppt SW. The results substantiate an inhibitory action of E(2) on hypoosmoregulatory capacity in this euryhaline teleost.
Authors:
Juan Miguel Mancera; Marcel Smolenaars; Raúl Laiz-Carrión; María del Pilar Martín del Río; S E Wendelaar Bonga; Gert Flik
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology     Volume:  139     ISSN:  1096-4959     ISO Abbreviation:  Comp. Biochem. Physiol. B, Biochem. Mol. Biol.     Publication Date:  2004 Oct 
Date Detail:
Created Date:  2004-10-06     Completed Date:  2005-06-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9516061     Medline TA:  Comp Biochem Physiol B Biochem Mol Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  183-91     Citation Subset:  IM    
Affiliation:
Departamento de Biología, Facultad de Ciencias del Mar y Ambientales, Universidad de Cádiz, Puerto Real, Cádiz 11510, Spain. juanmiguel.mancera@uca.es
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MeSH Terms
Descriptor/Qualifier:
4-Nitrophenylphosphatase / metabolism
Animals
Chlorides / blood
Dose-Response Relationship, Drug
Estradiol / administration & dosage,  pharmacology*
Fundulidae / physiology*
Gills / enzymology
Sodium / blood
Water-Electrolyte Balance / drug effects*
Chemical
Reg. No./Substance:
0/Chlorides; 50-28-2/Estradiol; 7440-23-5/Sodium; EC 3.1.3.41/4-Nitrophenylphosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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