Document Detail

17Beta-estradiol inhibits the adhesion of leukocytes in TNF-alpha stimulated human endothelial cells by blocking IL-8 and MCP-1 secretion, but not its transcription.
MedLine Citation:
PMID:  12204776     Owner:  NLM     Status:  MEDLINE    
Inflammation, and especially mononuclear cell adhesion to endothelium, is an important physiopathological component of atherosclerosis. Since coronary heart disease in women of reproductive age and/or with estrogen replacement therapy is reduced, our aim was to determine if 17beta-estradiol had a regulatory effect on the adhesion of lymphocytes to the endothelium. We performed U-937 cells adhesion assays in TNF-alpha-stimulated HUVECs, and we also quantitated IL-8 and MCP-1 in culture supernatants, in the presence or not of 17beta-estradiol. The presence of alpha- and beta-estrogen receptors was determined by Western blot and RT-PCR, respectively, whereas the transcription of both chemokines was evaluated by RT-PCR. The results showed a 35% decrease in the adhesion of U-937 monocyte cells to TNF-alpha-stimulated HUVECs, and a 54% and 65% inhibition of TNF-alpha-induced IL-8 and MCP-1 secretion by physiological and physiologically high doses of 17beta-estradiol. The hormone did not affect the transcription of both chemokine genes. Tamoxifen reverted the inhibitory effect induced by 17beta-estradiol. In conclusion, 17beta-estradiol modifies the adhesion of leukocytes to endothelial cells by inhibiting the secretion, but not the gene transcription, of proinflammatory chemokines.
Emma Rodríguez; Rebeca López; Araceli Paez; Felipe Massó; Luis F Montaño
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Life sciences     Volume:  71     ISSN:  0024-3205     ISO Abbreviation:  Life Sci.     Publication Date:  2002 Sep 
Date Detail:
Created Date:  2002-09-02     Completed Date:  2002-10-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375521     Medline TA:  Life Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  2181-93     Citation Subset:  IM    
Depto. Biología Celular, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano 1, Tlalpan 14080, D.F., México, Mexico.
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MeSH Terms
Blotting, Western
Cell Adhesion / drug effects
Cell Movement / drug effects
Cells, Cultured
Chemokine CCL2 / antagonists & inhibitors*,  biosynthesis,  metabolism
Chemotaxis, Leukocyte / drug effects
Endothelium, Vascular / cytology*,  drug effects
Estradiol / pharmacology*
Interleukin-8 / antagonists & inhibitors*,  biosynthesis,  metabolism
Leukocytes / drug effects*
RNA / biosynthesis,  genetics
Reverse Transcriptase Polymerase Chain Reaction
Transcription, Genetic / drug effects
Tumor Necrosis Factor-alpha / pharmacology*
Umbilical Veins / cytology,  drug effects
Reg. No./Substance:
0/Chemokine CCL2; 0/Interleukin-8; 0/Tumor Necrosis Factor-alpha; 50-28-2/Estradiol; 63231-63-0/RNA

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