Document Detail


15(S)-HETE modulates LTB(4) production and neutrophil chemotaxis in chronic bronchitis.
MedLine Citation:
PMID:  11003605     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We evaluated the levels of 15(S)-hydroxyeicosatetraenoic acid [15(S)-HETE] and the expression of 15-lipoxygenase (15-LO) mRNA in induced sputum obtained from 10 control and 15 chronic bronchitis subjects. 15(S)-HETE was evaluated by reverse phase high-performance liquid chromatography separation followed by specific RIA. 15-LO mRNA expression was determined by primed in situ labeling. The levels of both soluble and cell-associated 15(S)-HETE resulted significantly higher in chronic bronchitis than in control subjects. The percentage of cells expressing 15-LO mRNA was significantly higher in chronic bronchitis than in control subjects (P < 0.01). Double staining for specific cell type markers and 15-LO mRNA showed macrophages and neutrophils positive for 15-LO, whereas similar staining of peripheral blood neutrophils did not show evidence for 15-LO expression, suggesting that expression of 15-LO in neutrophils takes place on migration into the airways. Because 15(S)-HETE inversely correlated with the percentage of neutrophils in sputum of chronic bronchitis subjects, we studied the effect of 15(S)-HETE on leukotriene B(4) (LTB(4)) production in vitro and evaluated the concentration of LTB(4) in induced sputum and the contribution of LTB(4) to the chemotactic activity of induced sputum samples ex vivo. The results obtained indicate that macrophages and neutrophils present within the airways of chronic bronchitis subjects express 15-LO mRNA; increased basal levels of 15(S)-HETE may contribute to modulate, through the inhibition of 5-lipoxygenase metabolites production, neutrophil infiltration and airway inflammation associated with chronic bronchitis.
Authors:
M Profita; A Sala; L Riccobono; E Pace; A Paternò; S Zarini; L Siena; A Mirabella; G Bonsignore; A M Vignola
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Publication Detail:
Type:  Clinical Trial; Controlled Clinical Trial; Journal Article    
Journal Detail:
Title:  American journal of physiology. Cell physiology     Volume:  279     ISSN:  0363-6143     ISO Abbreviation:  Am. J. Physiol., Cell Physiol.     Publication Date:  2000 Oct 
Date Detail:
Created Date:  2000-10-12     Completed Date:  2000-10-30     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  100901225     Medline TA:  Am J Physiol Cell Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  C1249-58     Citation Subset:  IM    
Affiliation:
Istituto di Fisiopatologia Respiratoria, Consiglio Nazionale delle Ricerche, Palermo, Italy.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Arachidonate 15-Lipoxygenase / biosynthesis,  genetics
Bronchitis / metabolism*,  pathology
Cell Count
Cell Survival / drug effects
Cells, Cultured
Chemotaxis, Leukocyte / drug effects
Chronic Disease
Humans
Hydroxyeicosatetraenoic Acids / analysis,  metabolism*,  pharmacology
Immunohistochemistry
In Situ Hybridization
Ionophores / pharmacology
Leukotriene Antagonists / pharmacology
Leukotriene B4 / biosynthesis*
Lung Diseases, Obstructive / metabolism*,  pathology
Macrophages / metabolism
Middle Aged
Neutrophils / drug effects,  metabolism*,  pathology
RNA, Messenger / biosynthesis
Sputum / chemistry,  cytology,  metabolism
Chemical
Reg. No./Substance:
0/Hydroxyeicosatetraenoic Acids; 0/Ionophores; 0/Leukotriene Antagonists; 0/RNA, Messenger; 71160-24-2/Leukotriene B4; 73945-47-8/15-hydroxy-5,8,11,13-eicosatetraenoic acid; EC 1.13.11.33/Arachidonate 15-Lipoxygenase

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