| 15(S)-HETE modulates LTB(4) production and neutrophil chemotaxis in chronic bronchitis. | |
| | |
MedLine Citation:
|
PMID: 11003605 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We evaluated the levels of 15(S)-hydroxyeicosatetraenoic acid [15(S)-HETE] and the expression of 15-lipoxygenase (15-LO) mRNA in induced sputum obtained from 10 control and 15 chronic bronchitis subjects. 15(S)-HETE was evaluated by reverse phase high-performance liquid chromatography separation followed by specific RIA. 15-LO mRNA expression was determined by primed in situ labeling. The levels of both soluble and cell-associated 15(S)-HETE resulted significantly higher in chronic bronchitis than in control subjects. The percentage of cells expressing 15-LO mRNA was significantly higher in chronic bronchitis than in control subjects (P < 0.01). Double staining for specific cell type markers and 15-LO mRNA showed macrophages and neutrophils positive for 15-LO, whereas similar staining of peripheral blood neutrophils did not show evidence for 15-LO expression, suggesting that expression of 15-LO in neutrophils takes place on migration into the airways. Because 15(S)-HETE inversely correlated with the percentage of neutrophils in sputum of chronic bronchitis subjects, we studied the effect of 15(S)-HETE on leukotriene B(4) (LTB(4)) production in vitro and evaluated the concentration of LTB(4) in induced sputum and the contribution of LTB(4) to the chemotactic activity of induced sputum samples ex vivo. The results obtained indicate that macrophages and neutrophils present within the airways of chronic bronchitis subjects express 15-LO mRNA; increased basal levels of 15(S)-HETE may contribute to modulate, through the inhibition of 5-lipoxygenase metabolites production, neutrophil infiltration and airway inflammation associated with chronic bronchitis. |
| | |
Authors:
|
M Profita; A Sala; L Riccobono; E Pace; A Paternò; S Zarini; L Siena; A Mirabella; G Bonsignore; A M Vignola |
Related Documents
:
|
10615065 - Interleukin-8 gene repression by clarithromycin is mediated by the activator protein-1 ... 19184985 - Hepatocyte growth factor induces hypoxia-related interleukin-8 expression in lung adeno... 12622935 - Shear stress induces interleukin-8 mrna expression and transcriptional activation in hu... 18647315 - Rhinovirus infection and house dust mite exposure synergize in inducing bronchial epith... 23416275 - Modulation of p53 c-terminal acetylation by mdm2, p14arf and cytoplasmic sirt2. 18544455 - Interactions between gonadotropin-releasing hormone (gnrh) and orexin in the regulation... |
Publication Detail:
|
Type: Clinical Trial; Controlled Clinical Trial; Journal Article |
Journal Detail:
|
Title: American journal of physiology. Cell physiology Volume: 279 ISSN: 0363-6143 ISO Abbreviation: Am. J. Physiol., Cell Physiol. Publication Date: 2000 Oct |
Date Detail:
|
Created Date: 2000-10-12 Completed Date: 2000-10-30 Revised Date: 2004-11-17 |
Medline Journal Info:
|
Nlm Unique ID: 100901225 Medline TA: Am J Physiol Cell Physiol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: C1249-58 Citation Subset: IM |
Affiliation:
|
Istituto di Fisiopatologia Respiratoria, Consiglio Nazionale delle Ricerche, Palermo, Italy. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Aged Arachidonate 15-Lipoxygenase / biosynthesis, genetics Bronchitis / metabolism*, pathology Cell Count Cell Survival / drug effects Cells, Cultured Chemotaxis, Leukocyte / drug effects Chronic Disease Humans Hydroxyeicosatetraenoic Acids / analysis, metabolism*, pharmacology Immunohistochemistry In Situ Hybridization Ionophores / pharmacology Leukotriene Antagonists / pharmacology Leukotriene B4 / biosynthesis* Lung Diseases, Obstructive / metabolism*, pathology Macrophages / metabolism Middle Aged Neutrophils / drug effects, metabolism*, pathology RNA, Messenger / biosynthesis Sputum / chemistry, cytology, metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Hydroxyeicosatetraenoic Acids; 0/Ionophores; 0/Leukotriene Antagonists; 0/RNA, Messenger; 71160-24-2/Leukotriene B4; 73945-47-8/15-hydroxy-5,8,11,13-eicosatetraenoic acid; EC 1.13.11.33/Arachidonate 15-Lipoxygenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Transport and function of syntaxin 3 in human epithelial intestinal cells.
Next Document: Sterol carrier protein-2 localization in endoplasmic reticulum and role in phospholipid formation.