Document Detail


A 150-megadalton plasmid in Rhizobium etli strain TAL182 contains genes for nodulation competitiveness on Phaseolus vulgaris L.
MedLine Citation:
PMID:  8864213     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Rhizobium etli TAL182, a competitive strain for the nodulation of Phaseolus beans, occupied more than 99% of the nodules when co-inoculated in various proportions with Rhizobium TAL1145 or Rhizobium tropici CIAT899. Two overlapping cosmid clones, pUHR68 and pUHR69, containing genes for nodulation competitiveness from TAL182, were isolated by functional complementation of strain TAL1145. Using one of these cosmid clones, we constructed two Tn5-insertion mutants of TAL182 defective in nodulation competitiveness. The Tn5 insertions in both mutants were localized in identical positions within a 4.6-kb HindIII fragment. One mutant, RUH120, was complemented for nodulation competitiveness by this HindIII fragment. The cloned DNA in pUHR68 is a part of a plasmid, 150 MDa in size, in TAL182 and does not show homology with TAL1145 genomic DNA. The 4.6-kb HindIII fragment contains a gene(s) required for nodulation competitiveness on beans, which is present only in some R. etli strains and absent in other Rhizobium spp.
Authors:
D Borthakur; X Gao
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Canadian journal of microbiology     Volume:  42     ISSN:  0008-4166     ISO Abbreviation:  Can. J. Microbiol.     Publication Date:  1996 Sep 
Date Detail:
Created Date:  1996-11-21     Completed Date:  1996-11-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372707     Medline TA:  Can J Microbiol     Country:  CANADA    
Other Details:
Languages:  eng     Pagination:  903-10     Citation Subset:  IM    
Affiliation:
Department of Plant Molecular Physiology, University of Hawaii, Honolulu, 96822, USA, dulal@hawaii.edu
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MeSH Terms
Descriptor/Qualifier:
Cosmids / genetics
DNA Transposable Elements / genetics
Fabaceae / microbiology*
Genes, Bacterial / physiology*
Genetic Complementation Test
Mutagenesis, Site-Directed
Plant Roots / microbiology
Plants, Medicinal*
Plasmids / genetics*
Restriction Mapping
Rhizobium / genetics*
Sequence Homology, Nucleic Acid
Chemical
Reg. No./Substance:
0/DNA Transposable Elements

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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