| 15-deoxy-(Delta12,14)-prostaglandin J2 (15d-PGJ2) induces cell death through caspase-independent mechanism in A172 human glioma cells. | |
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MedLine Citation:
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PMID: 17006759 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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15-Deoxy-(Delta12,14)-prostaglandin J(2) (15d-PGJ(2)) is a naturally occurring cyclopentenone metabolite of prostaglandin D(2) (PGD(2)) and is known as a specific potent ligand for the peroxisome proliferators activator receptor-gamma (PPARgamma). 15d-PGJ(2) inhibits cell growth and induces apoptosis in a number of different cancer cells. However, the underlying mechanism by which 15d-PGJ(2) induces cell death remains to be defined. The present study was undertaken to determine the effect of 15d-PGJ(2) on cell death in A172 human glioma cells. 15d-PGJ(2) caused reactive oxygen species (ROS) generation. 15d-PGJ(2)-induced ROS production and cell death were prevented by the antioxidant N-acetylcysteine. Activation of mitogen-activated protein kinases (MAPK) was not observed in cells treated with 15d-PGJ(2 )and inhibitors of MAPK subfamilies also were not effective in preventing 15d-PGJ(2)-induced cell death. 15d-PGJ(2) treatment caused mitochondrial dysfunction, as evidenced by depolarization of mitochondrial membrane potential. 15d-PGJ(2) induced caspase activation at 24 h of treatment, but the 15d-PGJ(2)-induced cell death was not prevented by caspase inhibitors. The antiapoptotic protein XIAP levels and release of apoptosis inducing factor (AIF) into the cytosol were not altered by 15d-PGJ(2) treatment. Taken together, these findings indicate that 15d-PGJ(2) triggers cell death through a caspase-independent mechanism and ROS production and disruption of mitochondrial membrane potential play an important role in the 15d-PGJ(2)-induced cell death in A172 human glioma cells. |
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Authors:
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W H Cho; C H Choi; J Y Park; S K Kang; Y K Kim |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Neurochemical research Volume: 31 ISSN: 0364-3190 ISO Abbreviation: Neurochem. Res. Publication Date: 2006 Oct |
Date Detail:
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Created Date: 2006-11-03 Completed Date: 2007-01-24 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 7613461 Medline TA: Neurochem Res Country: United States |
Other Details:
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Languages: eng Pagination: 1247-54 Citation Subset: IM |
Affiliation:
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Department of Neurosurgery, College of Medicine, Pusan National University, Pusan, 602-739, Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Brain Neoplasms
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enzymology,
metabolism,
pathology* Caspases / metabolism Cell Death / drug effects* Cell Line, Tumor Glioma / enzymology, metabolism, pathology* Humans Mitogen-Activated Protein Kinases / antagonists & inhibitors, metabolism Prostaglandin D2 / analogs & derivatives*, pharmacology Reactive Oxygen Species / metabolism |
| Chemical | |
Reg. No./Substance:
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0/15-deoxyprostaglandin J2; 0/Reactive Oxygen Species; 41598-07-6/Prostaglandin D2; EC 2.7.11.24/Mitogen-Activated Protein Kinases; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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