Document Detail

1.5 MHz ultrasound irradiation of human lymphocytes.
MedLine Citation:
PMID:  312789     Owner:  NLM     Status:  MEDLINE    
Human lymphocyte cultures are exposed to 1.5 MHz continuous wave ultrasound, and it is demonstrated that cell death, as monitored by pyknosis, follows immediately on sonication at intensities within the usual therapeutic range (less than 1.7 W/cm2,spatial average). The number of cells affected is determined by the ultrasound intensity only, but the rate at which they proceed through their pyknosis cycle is modified by both the intensity and the duration of exposure. There is a clear indication of an intensity threshold for the effect approximately 1.1 W/cm2. Pulsed 1.5 MHz ultrasound (70 mus, 1 :1 pulses, 1.7 W/cm2 space-time average) results in a 15-20 hour delay in the measurable response to sonication. It is shown that the intracellular presence of a lysosomal-enzyme inhibitor strongly modifies the course of the ultrasound action. Evidence is presented to suggest that the basic interaction mechanism is via a cavitation process, but there are some difficulties with this interpretation, which are also discussed.
M J Hedges; S Leeman
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  International journal of radiation biology and related studies in physics, chemistry, and medicine     Volume:  35     ISSN:  0020-7616     ISO Abbreviation:  Int. J. Radiat. Biol. Relat. Stud. Phys. Chem. Med.     Publication Date:  1979 Apr 
Date Detail:
Created Date:  1979-08-29     Completed Date:  1979-08-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0374725     Medline TA:  Int J Radiat Biol Relat Stud Phys Chem Med     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  301-11     Citation Subset:  IM    
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MeSH Terms
Cell Nucleus
Cell Survival
Cells, Cultured
Cortisone / pharmacology
Lymphocytes / pathology*,  ultrastructure
Lysosomes / enzymology
Sonication / adverse effects*
Ultrasonic Therapy / adverse effects
Ultrasonics / adverse effects*
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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