Document Detail


15-HETE suppresses K(+) channel activity and inhibits apoptosis in pulmonary artery smooth muscle cells.
MedLine Citation:
PMID:  19082729     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
15-Hydroxyeicosatetraenoic acid (15-HETE) is an important hypoxic product from arachidonic acid (AA) in the wall of pulmonary vessels. Although its effects on pulmonary artery constriction are well known, it remains unclear whether 15-HETE acts on the apoptotic responses in pulmonary artery smooth muscle cells (PASMCs) and whether K(+) channels participate in this process. These hypothesises were validated by cell viability assay, terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling, mitochondrial potentials assay, caspase activity assay and western blot. We found that 15-HETE enhanced cell survival, suppressed the expression and activity of caspase-3, upregulated bcl-2 and attenuated mitochondrial depolarization, prevented chromatin condensation and partly reversed K(+) channel opener-induced apoptosis in PASMCs under serum-deprived conditions. Our data indicated that 15-HETE inhibits the apoptosis in PASMCs through, at least in part, inactivating K(+) channels.
Authors:
Yumei Li; Qian Li; Zhigang Wang; Di Liang; Shujun Liang; Xiaobo Tang; Lei Guo; Rong Zhang; Daling Zhu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Apoptosis : an international journal on programmed cell death     Volume:  14     ISSN:  1573-675X     ISO Abbreviation:  Apoptosis     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2008-12-23     Completed Date:  2009-01-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9712129     Medline TA:  Apoptosis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  42-51     Citation Subset:  IM    
Affiliation:
Department of Biopharmaceutical Sciences, College of Pharmacy, Harbin Medical University, 157 Baojian Road, Nangang District, Harbin, 150081, Heilongjiang, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Anoxia
Apoptosis*
Arachidonic Acid / chemistry
Caspase 3 / metabolism
Humans
Hydrogen Peroxide / chemistry
Hydroxyeicosatetraenoic Acids / pharmacology*
Mitochondria / metabolism
Models, Biological
Myocytes, Smooth Muscle / metabolism*
Potassium / chemistry
Potassium Channels / chemistry*
Proto-Oncogene Proteins c-bcl-2 / metabolism
Pulmonary Artery / pathology*
Tetrazolium Salts / pharmacology
Thiazoles / pharmacology
Chemical
Reg. No./Substance:
0/Hydroxyeicosatetraenoic Acids; 0/Potassium Channels; 0/Proto-Oncogene Proteins c-bcl-2; 0/Tetrazolium Salts; 0/Thiazoles; 298-93-1/thiazolyl blue; 506-32-1/Arachidonic Acid; 73945-47-8/15-hydroxy-5,8,11,13-eicosatetraenoic acid; 7440-09-7/Potassium; 7722-84-1/Hydrogen Peroxide; EC 3.4.22.-/Caspase 3

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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