Document Detail

A 13C mass isotopomer study of anaplerotic pyruvate carboxylation in perfused rat hearts.
MedLine Citation:
PMID:  9334177     Owner:  NLM     Status:  MEDLINE    
Anaplerotic pyruvate carboxylation was examined in hearts perfused with physiological concentrations of glucose, [U-13C3]lactate, and [U-13C3]pyruvate. Also, a fatty acid, [1-13C]octanoate, or ketone bodies were added at concentrations providing acetyl-CoA at a rate resulting in either low or substantial pyruvate decarboxylation. Relative contributions of pyruvate and fatty acids to citrate synthesis were determined from the 13C labeling pattern of effluent citrate by gas chromatography-mass spectrometry (see companion article, Comte, B., Vincent, G., Bouchard, B., and Des Rosiers, C. (1997) J. Biol. Chem. 272, 26117-26124). Precision on flux measurements of anaplerotic pyruvate carboxylation depended on the mix of substrates supplied to the heart. Anaplerotic fluxes were precisely determined under conditions where acetyl-CoA was predominantly supplied by beta-oxidation, as it occurred with 0.2 or 1 mM octanoate. Then, anaplerotic pyruvate carboxylation provided 3-8% of the OAA moiety of citrate and was modulated by concentrations of lactate and pyruvate in the physiological range. Also, the contribution of pyruvate to citrate formation through carboxylation was equal to or greater than through decarboxylation. Furthermore, 13C labeling data on tissue citric acid cycle intermediates and pyruvate suggest that (i) anaplerosis occurs also at succinate and (ii) cataplerotic malate decarboxylation is low. Rather, the presence of citrate in the effluent perfusate of hearts perfused with physiological concentrations of glucose, lactate, and pyruvate and concentrations of octanoate leading to maximal oxidative rates suggests a cataplerotic citrate efflux from mitochondria to cytosol. Taken altogether, our data raise the possibility of a link between pyruvate carboxylation and mitochondrial citrate efflux. In view of the proposed feedback regulation of glycolysis by cytosolic citrate, such a link would support a role of anaplerosis and cataplerosis in metabolic signal transmission between mitochondria and cytosol in the normoxic heart.
B Comte; G Vincent; B Bouchard; M Jetté; S Cordeau; C D Rosiers
Related Documents :
24167657 - A novel cutaneous fatty acid-binding protein-related signaling pathway leading to malig...
21128717 - Stealth-nanoparticle strategy for enhancing the efficacy of steroids in mice with noise...
17602527 - Synthesis of deoxy sugar esters: a chemoenzymatic stereoselective approach affording de...
8300577 - Effect of fatty acids on h+ transport activity of the reconstituted uncoupling protein.
6547407 - Cyclic peptides. xvi. syntheses of am-toxin i analogs containing a lower or higher homo...
13688207 - A sensitive method for the colorimetric determination of urea.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  272     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1997 Oct 
Date Detail:
Created Date:  1997-11-17     Completed Date:  1997-11-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  26125-31     Citation Subset:  IM    
Department of Nutrition, University of Montréal, Montréal, Québec H3C 3J7, Canada.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Carbon Isotopes
Carboxylic Acids / metabolism
Gas Chromatography-Mass Spectrometry
Myocardium / metabolism*
Pyruvic Acid / metabolism*
Reg. No./Substance:
0/Carbon Isotopes; 0/Carboxylic Acids; 127-17-3/Pyruvic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Probing the origin of acetyl-CoA and oxaloacetate entering the citric acid cycle from the 13C labeli...
Next Document:  Identification, cloning, and mutational analysis of the casein kinase 1 cDNA of the malaria parasite...