Document Detail


13C isotopomer model for estimation of anaplerotic substrate oxidation via acetyl-CoA.
MedLine Citation:
PMID:  8897869     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A previous model using 13C nuclear magnetic resonance isotopomer analysis provided for direct measurement of the oxidation of 13C-enriched substrates in the tricarboxylic acid cycle and/or their entry via anaplerotic pathways. This model did not allow for recycling of labeled metabolites from tricarboxylic acid cycle intermediates into the acetyl-CoA pool. An extension of this model is now presented that incorporates carbon flow from oxaloacetate or malate to acetyl-CoA. This model was examined using propionate metabolism in the heart, in which previous observations indicated that all of the propionate consumed was oxidized to CO2 and water. Application of the new isotopomer model shows that 2 mM [3-13C]propionate entered the tricarboxylic acid cycle as succinyl-CoA (an anaplerotic pathway) at a rate equal to 52% of tricarboxylic acid cycle turnover and that all of this carbon entered the acetyl-CoA pool and was oxidized. This was verified using standard biochemical analysis; from the rate (mumol.min-1.g dry wt-1) of propionate uptake (4.0 +/- 0.7), the estimated oxygen consumption (24.8 +/- 5) matched that experimentally determined (24.4 +/- 3).
Authors:
F M Jeffrey; C J Storey; A D Sherry; C R Malloy
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  271     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1996 Oct 
Date Detail:
Created Date:  1996-12-16     Completed Date:  1996-12-16     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  E788-99     Citation Subset:  IM    
Affiliation:
Department of Radiology, University of Texas Southwestern Medical Center, Dallas, USA.
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MeSH Terms
Descriptor/Qualifier:
Acetyl Coenzyme A / metabolism*
Animals
Aspartic Acid / metabolism
Carbon Isotopes
Citric Acid Cycle
Glutamic Acid / metabolism
Magnetic Resonance Spectroscopy
Male
Models, Biological
Oxaloacetates / metabolism
Oxidation-Reduction
Propionates / metabolism
Rats
Rats, Sprague-Dawley
Grant Support
ID/Acronym/Agency:
HL-17669-16/HL/NHLBI NIH HHS; HL-34557/HL/NHLBI NIH HHS; RR-02584/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Carbon Isotopes; 0/Oxaloacetates; 0/Propionates; 56-84-8/Aspartic Acid; 56-86-0/Glutamic Acid; 72-89-9/Acetyl Coenzyme A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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