Document Detail


A (13)C NMR double-labeling method to quantitate local myocardial O(2) consumption using frozen tissue samples.
MedLine Citation:
PMID:  10516204     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Measurement of local myocardial O(2) consumption (VO(2)) has been problematic but is needed to investigate the heterogeneity of aerobic metabolism. The goal of the present investigation was to develop a method to measure local VO(2) using small frozen myocardial samples, suitable for determining VO(2) profiles. In 26 isolated rabbit hearts, 1.5 mmol/l [2-(13)C]acetate was infused for 4 min, followed by 1.5 min of [1,2-(13)C]acetate. The left ventricular (LV) free wall was then quickly frozen. High-resolution (13)C-NMR spectra were measured from extracts taken from 2- to 3-mm thick transmural layer samples. The multiplet intensities of glutamate were analyzed with a computer model allowing simultaneous estimation of the absolute flux through the tricarboxylic acid cycle and the fractional contribution of acetate to acetyl CoA formation from which local VO(2) was calculated. The (13)C-derived VO(2) in the LV free wall was linearly related to "gold standard" VO(2) from coronary venous O(2) electrode measurements in the same region (r = 0.932, n = 22, P < 0.0001, slope 1.05) for control and lowered metabolic rates. The ratio of subendocardial to subepicardial VO(2) was 1.52 +/- 0.19 (SE, significantly >1, P < 0.025). Local myocardial VO(2) can now be quantitated with this new (13)C method to determine profiles of aerobic energy metabolism.
Authors:
J H van Beek; H G van Mil; R B King; F J de Kanter; D J Alders; J Bussemaker
Related Documents :
8465794 - Left ventricular myocardial function in myotonic dystrophy.
16188524 - Outcomes by race and etiology of patients with left ventricular systolic dysfunction.
9462414 - Effects of left ventricular chamber size and left ventricular diastolic pressure on lef...
17885184 - Prediction of left ventricular remodeling and analysis of infarct resorption in patient...
24774934 - Ionic currents and ventricular fibrillation dynamics.
24275554 - Growth/differentiation factor 1 alleviates pressure overload-induced cardiac hypertroph...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  277     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1999 Oct 
Date Detail:
Created Date:  1999-11-17     Completed Date:  1999-11-17     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  H1630-40     Citation Subset:  IM    
Affiliation:
Laboratory for Physiology, Vrije Universiteit, 1081 BT Amsterdam, The Netherlands. vanbeek@physiol.med.vu.nl
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Carbon Isotopes
Computer Simulation
Endocardium / metabolism
Freezing
Magnetic Resonance Spectroscopy
Models, Cardiovascular
Myocardium / metabolism*
Oxygen Consumption*
Pericardium / metabolism
Rabbits
Grant Support
ID/Acronym/Agency:
RR-01243/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Carbon Isotopes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Potassium channel-mediated vasorelaxation is impaired in experimental renal failure.
Next Document:  TNF-alpha enhances cardiac myocyte NO production through MAP kinase-mediated NF-kappaB activation.