Document Detail

A (13)C NMR double-labeling method to quantitate local myocardial O(2) consumption using frozen tissue samples.
MedLine Citation:
PMID:  10516204     Owner:  NLM     Status:  MEDLINE    
Measurement of local myocardial O(2) consumption (VO(2)) has been problematic but is needed to investigate the heterogeneity of aerobic metabolism. The goal of the present investigation was to develop a method to measure local VO(2) using small frozen myocardial samples, suitable for determining VO(2) profiles. In 26 isolated rabbit hearts, 1.5 mmol/l [2-(13)C]acetate was infused for 4 min, followed by 1.5 min of [1,2-(13)C]acetate. The left ventricular (LV) free wall was then quickly frozen. High-resolution (13)C-NMR spectra were measured from extracts taken from 2- to 3-mm thick transmural layer samples. The multiplet intensities of glutamate were analyzed with a computer model allowing simultaneous estimation of the absolute flux through the tricarboxylic acid cycle and the fractional contribution of acetate to acetyl CoA formation from which local VO(2) was calculated. The (13)C-derived VO(2) in the LV free wall was linearly related to "gold standard" VO(2) from coronary venous O(2) electrode measurements in the same region (r = 0.932, n = 22, P < 0.0001, slope 1.05) for control and lowered metabolic rates. The ratio of subendocardial to subepicardial VO(2) was 1.52 +/- 0.19 (SE, significantly >1, P < 0.025). Local myocardial VO(2) can now be quantitated with this new (13)C method to determine profiles of aerobic energy metabolism.
J H van Beek; H G van Mil; R B King; F J de Kanter; D J Alders; J Bussemaker
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  277     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1999 Oct 
Date Detail:
Created Date:  1999-11-17     Completed Date:  1999-11-17     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  H1630-40     Citation Subset:  IM    
Laboratory for Physiology, Vrije Universiteit, 1081 BT Amsterdam, The Netherlands.
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MeSH Terms
Carbon Isotopes
Computer Simulation
Endocardium / metabolism
Magnetic Resonance Spectroscopy
Models, Cardiovascular
Myocardium / metabolism*
Oxygen Consumption*
Pericardium / metabolism
Grant Support
Reg. No./Substance:
0/Carbon Isotopes

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