Document Detail


[13C] GC-C-IRMS analysis of methylboronic acid derivatives of glucose from liver glycogen after the ingestion of [13C] labeled tracers in rats.
MedLine Citation:
PMID:  19775945     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We developed a complete method to measure low [(13)C] enrichments in glycogen. Fourteen rats were fed a control diet. Six of them also ingested either [U-(13)C] glucose (n=2) or a mixture of 20 [U-(13)C] amino acids (n=4). Hepatic glycogen was extracted, digested to glucose and purified on anion-cation exchange resins. After the optimization of methylboronic acid derivatization using GC-MS, [(13)C] enrichment of extracted glucose was measured by GC-C-IRMS. The accuracy was addressed by measuring the enrichment excess of a calibration curve, which observed values were in good agreement with the expected values (R=0.9979). Corrected delta values were -15.6+/-1.6 delta(13)C (per thousand) for control rats (n=8) and increased to -5 to 8 delta(13)C (per thousand) per thousand and 12-14 delta(13)C (per thousand) per thousand after the ingestion of [U-(13)C] amino acids or [U-(13)C] glucose as oral tracers, respectively. The method enabled the determination of dietary substrate transfer into glycogen. The sequestration of dietary glucose in liver glycogen 4 h after the meal was 35% of the ingested dose whereas the transfer of carbon skeletons from amino acids was only 0.25 to 1%.
Authors:
Catherine Luengo; Dalila Azzout-Marniche; Claire Fromentin; Julien Piedcoq; Sophie Lemosquet; Daniel Tom??; Claire Gaudichon
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Publication Detail:
Type:  Journal Article     Date:  2009-09-10
Journal Detail:
Title:  Journal of chromatography. B, Analytical technologies in the biomedical and life sciences     Volume:  877     ISSN:  1873-376X     ISO Abbreviation:  J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-10-12     Completed Date:  2009-12-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101139554     Medline TA:  J Chromatogr B Analyt Technol Biomed Life Sci     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  3638-44     Citation Subset:  IM    
Affiliation:
INRA, CNRH-IdF, UMR914 Nutrition Physiology and Ingestive Behavior, F-75005 Paris, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Boron Compounds / chemistry*
Carbon Isotopes / analysis*
Gas Chromatography-Mass Spectrometry / methods*
Glucose / chemistry*
Liver Glycogen / chemistry,  metabolism*
Male
Molecular Structure
Rats
Rats, Wistar
Chemical
Reg. No./Substance:
0/Boron Compounds; 0/Carbon Isotopes; 0/Liver Glycogen; 0/methaneboronic acid; 50-99-7/Glucose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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