| [13C] GC-C-IRMS analysis of methylboronic acid derivatives of glucose from liver glycogen after the ingestion of [13C] labeled tracers in rats. | |
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MedLine Citation:
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PMID: 19775945 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We developed a complete method to measure low [(13)C] enrichments in glycogen. Fourteen rats were fed a control diet. Six of them also ingested either [U-(13)C] glucose (n=2) or a mixture of 20 [U-(13)C] amino acids (n=4). Hepatic glycogen was extracted, digested to glucose and purified on anion-cation exchange resins. After the optimization of methylboronic acid derivatization using GC-MS, [(13)C] enrichment of extracted glucose was measured by GC-C-IRMS. The accuracy was addressed by measuring the enrichment excess of a calibration curve, which observed values were in good agreement with the expected values (R=0.9979). Corrected delta values were -15.6+/-1.6 delta(13)C (per thousand) for control rats (n=8) and increased to -5 to 8 delta(13)C (per thousand) per thousand and 12-14 delta(13)C (per thousand) per thousand after the ingestion of [U-(13)C] amino acids or [U-(13)C] glucose as oral tracers, respectively. The method enabled the determination of dietary substrate transfer into glycogen. The sequestration of dietary glucose in liver glycogen 4 h after the meal was 35% of the ingested dose whereas the transfer of carbon skeletons from amino acids was only 0.25 to 1%. |
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Authors:
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Catherine Luengo; Dalila Azzout-Marniche; Claire Fromentin; Julien Piedcoq; Sophie Lemosquet; Daniel Tom??; Claire Gaudichon |
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Publication Detail:
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Type: Journal Article Date: 2009-09-10 |
Journal Detail:
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Title: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Volume: 877 ISSN: 1873-376X ISO Abbreviation: J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. Publication Date: 2009 Nov |
Date Detail:
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Created Date: 2009-10-12 Completed Date: 2009-12-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101139554 Medline TA: J Chromatogr B Analyt Technol Biomed Life Sci Country: Netherlands |
Other Details:
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Languages: eng Pagination: 3638-44 Citation Subset: IM |
Affiliation:
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INRA, CNRH-IdF, UMR914 Nutrition Physiology and Ingestive Behavior, F-75005 Paris, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Boron Compounds / chemistry* Carbon Isotopes / analysis* Gas Chromatography-Mass Spectrometry / methods* Glucose / chemistry* Liver Glycogen / chemistry, metabolism* Male Molecular Structure Rats Rats, Wistar |
| Chemical | |
Reg. No./Substance:
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0/Boron Compounds; 0/Carbon Isotopes; 0/Liver Glycogen; 0/methaneboronic acid; 50-99-7/Glucose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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