Document Detail


β(1,3)-glucanosyl-transferase activity is essential for cell wall integrity and viability of Schizosaccharomyces pombe.
MedLine Citation:
PMID:  21124977     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The formation of the cell wall in Schizosaccharomyces pombe requires the coordinated activity of enzymes involved in the biosynthesis and modification of β-glucans. The β(1,3)-glucan synthase complex synthesizes linear β(1,3)-glucans, which remain unorganized until they are cross-linked to other β(1,3)-glucans and other cell wall components. Transferases of the GH72 family play important roles in cell wall assembly and its rearrangement in Saccharomyces cerevisiae and Aspergillus fumigatus. Four genes encoding β(1,3)-glucanosyl-transferases -gas1(+), gas2(+), gas4(+) and gas5(+)- are present in S. pombe, although their function has not been analyzed.
METHODOLOGY/PRINCIPAL FINDINGS: Here, we report the characterization of the catalytic activity of gas1p, gas2p and gas5p together with studies directed to understand their function during vegetative growth. From the functional point of view, gas1p is essential for cell integrity and viability during vegetative growth, since gas1Δ mutants can only grow in osmotically supported media, while gas2p and gas5p play a minor role in cell wall construction. From the biochemical point of view, all of them display β(1,3)-glucanosyl-transferase activity, although they differ in their specificity for substrate length, cleavage point and product size. In light of all the above, together with the differences in expression profiles during the life cycle, the S. pombe GH72 proteins may accomplish complementary, non-overlapping functions in fission yeast.
CONCLUSIONS/SIGNIFICANCE: We conclude that β(1,3)-glucanosyl-transferase activity is essential for viability in fission yeast, being required to maintain cell integrity during vegetative growth.
Authors:
María de Medina-Redondo; Yolanda Arnáiz-Pita; Cécile Clavaud; Thierry Fontaine; Francisco del Rey; Jean Paul Latgé; Carlos R Vázquez de Aldana
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-11-18
Journal Detail:
Title:  PloS one     Volume:  5     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2010  
Date Detail:
Created Date:  2010-12-02     Completed Date:  2011-04-27     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e14046     Citation Subset:  IM    
Affiliation:
Departamento de Microbiología y Genética, Instituto de Microbiología Bioquímica, Consejo Superior de Investigaciones Científicas, Universidad de Salamanca, Salamanca, Spain.
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MeSH Terms
Descriptor/Qualifier:
Biocatalysis
Blotting, Northern
Cell Cycle / genetics,  physiology
Cell Division / genetics,  physiology
Cell Wall / metabolism*
Gene Expression Regulation, Fungal
Glucan Endo-1,3-beta-D-Glucosidase / genetics,  metabolism*
Glucans / metabolism
Isoenzymes / genetics,  metabolism
Luminescent Proteins / genetics,  metabolism
Microscopy, Fluorescence
Microscopy, Phase-Contrast
Mutation
Schizosaccharomyces / enzymology*,  genetics,  growth & development
Schizosaccharomyces pombe Proteins / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Glucans; 0/Isoenzymes; 0/Luminescent Proteins; 0/Schizosaccharomyces pombe Proteins; EC 3.2.1.-/1,3-beta-glucanosyltransferase; EC 3.2.1.39/Glucan Endo-1,3-beta-D-Glucosidase
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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