Document Detail

13-C nuclear magnetic resonance studies on the lipid organization in enveloped virions (vesicular stomatitis virus).
MedLine Citation:
PMID:  167809     Owner:  NLM     Status:  MEDLINE    
13-C nuclear magnetic resonance (NMR) studies are described regarding the lipid organization in the envelope of the vesicular stomatitis virion. The fatty acid chains (oleic acid) and the choline moiety of the 3-sn-phosphatidylcholine and spingomyelin have been labeled specifically with 13-C by growing the virions in prelabeled host cells (BHK 21 cells). The results suggest that 130C NMR spectroscopy is a very feasible method for the study of natural membranes provided the isotope is highly enriched in specific positions and incorporated biochemically. Spin-lattice relaxation (T1) measurements of particular C atoms have been carried out with whole virions, with virions deprived of their surface projections by trypsinization but unaltered in their shape and size, and with liposomes prepared from the total lipid mixture of the envelope in order to get insight into the molecular structure of this model membrane. The mobility of the central part of 11-13-C-labeled oleic acid incorporated into the ester and amide lipids and the choline group of 3-sn-phosphatidylcholine and sphingomyelin is very restricted as indicated by their short T1 times. It is concluded from the data presented here that the high cholesterol content (cholesterol/P: 0.7) of the envelope lipid phase is responsible for the rather rigidly packed envelope structure. The mode and extent of the interactions between lipids and glycoprotein surface projections are subjects for further study.
W Stoffel; K Bister
Related Documents :
8744839 - Effect of two cardiac glycosides, digitoxin and digoxin on blood lipids.
7442709 - The incorporation and utilization of radiolabelled lipids by adult schistosoma mansoni ...
11378469 - Lipoxygenase-dependent degradation of storage lipids.
2833499 - Lipid a precursor from pseudomonas aeruginosa is completely acylated prior to addition ...
22336199 - Rate and breadth of protein evolution are only weakly correlated.
18489559 - Organic nitrogen of tomato waste hydrolysate enhances glucose uptake and lipid accumula...
9430459 - Trapping and metabolism of radioiodinated phipa 3-10 in the rat myocardium.
24808729 - A new species of graphis and new lichen records from vietnam, including a second worldw...
11894099 - An amino-acid taste receptor.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochemistry     Volume:  14     ISSN:  0006-2960     ISO Abbreviation:  Biochemistry     Publication Date:  1975 Jul 
Date Detail:
Created Date:  1975-11-08     Completed Date:  1975-11-08     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0370623     Medline TA:  Biochemistry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2841-7     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Carbon Isotopes
Cell Line
Cholesterol / physiology
Choline / metabolism
Chromatography, Thin Layer
Lipids / analysis*
Liposomes / analysis
Magnetic Resonance Spectroscopy
Membranes / analysis
Microscopy, Electron
Oleic Acids / metabolism
Phosphatidylcholines / analysis
Spectrum Analysis
Sphingomyelins / analysis
Trypsin / pharmacology
Vesicular stomatitis Indiana virus / analysis*,  isolation & purification,  ultrastructure
Reg. No./Substance:
0/Carbon Isotopes; 0/Lipids; 0/Liposomes; 0/Oleic Acids; 0/Phosphatidylcholines; 0/Sphingomyelins; 57-88-5/Cholesterol; 62-49-7/Choline; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Swine lipoproteins and atherosclerosis. Changes in the plasma lipoproteins and apoproteins induced b...
Next Document:  The amino acid sequence of chick skin collagen alpha1-CB7.