| 1,25-dihydroxyvitamin D3 modulates cytokine production induced by Candida albicans: impact of seasonal variation of immune responses. | |
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MedLine Citation:
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PMID: 21148505 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Our interest in immunological effects produced by vitamin D(3) (1,25(OH)(2)D(3)) and its therapeutic potential prompted us to examine the role of 1,25(OH)(2)D(3) on cytokine production by Candida albicans. METHODS: Peripheral blood mononuclear cells (PBMC) with stimulated C. albicans and 1,25(OH)(2)D(3), cytokine concentrations were measured in supernatant. Quantitative polymerase chain reaction (qPCR) was performed for T cell transcription factors, SOCS1 and 3. TLR2/4, Dectin-1, and mannose receptor expression was studied using flow cytometry and qPCR. An ex-vivo stimulation study was carried out in healthy volunteers to investigate the seasonality of immune response to C. albicans. RESULTS: Upon in vitro C. albicans stimulation, 1,25(OH)(2)D(3) induced a dose-dependent, down-regulation of IL-6, TNFα, IL-17, and IFNγ. It also increased IL-10 production. The shift in cytokine profile was not due to 1,25(OH)(2)D(3) augmenting expression of either Thelper differentiation factors or SOCS1 and SOCS3 mRNA. 1,25(OH)(2)D(3) inhibited TLR2, TLR4, Dectin-1, and MR mRNA and protein expression. In our seasonality study, both IL-17 and IFNγ levels were suppressed in summer when 25(OH)D(3) levels were elevated. CONCLUSION: Vitamin D(3) skews cytokine responses toward an antiinflammatory profile, mediated by suppression of TLR2, TLR4, Dectin-1, and MR transcription, leading to reduced surface expression. The biological relevance of these effects has been confirmed by the seasonality of cytokine responses. |
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Authors:
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Ai-Leng Khoo; Louis Y A Chai; Hans J P M Koenen; Bart-Jan Kullberg; Irma Joosten; André J A M van der Ven; Mihai G Netea |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of infectious diseases Volume: 203 ISSN: 1537-6613 ISO Abbreviation: J. Infect. Dis. Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2010-12-14 Completed Date: 2011-01-11 Revised Date: 2012-01-02 |
Medline Journal Info:
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Nlm Unique ID: 0413675 Medline TA: J Infect Dis Country: United States |
Other Details:
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Languages: eng Pagination: 122-30 Citation Subset: AIM; IM |
Affiliation:
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Department of Laboratory Medicine, Radboud University Nijmegen Medical Center; 3Nijmegen Institute for Infection, Inflammation and Immunity, Nijmegen, The Netherlands. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Calcitriol / pharmacology* Candida albicans / immunology* Cells, Cultured Cytokines / secretion* Flow Cytometry Gene Expression Profiling Human Experimentation Humans Immunologic Factors / pharmacology* Lectins, C-Type / analysis Leukocytes, Mononuclear / chemistry, drug effects, immunology, microbiology Male Mannose-Binding Lectins / analysis Membrane Proteins / analysis Middle Aged Nerve Tissue Proteins / analysis Receptors, Cell Surface / analysis Reverse Transcriptase Polymerase Chain Reaction Seasons Suppressor of Cytokine Signaling Proteins / analysis Toll-Like Receptor 2 / analysis Toll-Like Receptor 4 / analysis |
| Chemical | |
Reg. No./Substance:
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0/Cytokines; 0/Immunologic Factors; 0/Lectins, C-Type; 0/Mannose-Binding Lectins; 0/Membrane Proteins; 0/Nerve Tissue Proteins; 0/Receptors, Cell Surface; 0/SOCS1 protein, human; 0/SOCS3 protein, human; 0/Suppressor of Cytokine Signaling Proteins; 0/Toll-Like Receptor 2; 0/Toll-Like Receptor 4; 0/dectin 1; 0/mannose receptor; 32222-06-3/Calcitriol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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