| 1α,25-Dihydroxyvitamin D(3) on intestinal transporter function: studies with the rat everted intestinal sac. | |
| | |
MedLine Citation:
|
PMID: 21341280 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
Previous studies have shown that 1α,25-dihydroxyvitamin D(3) (1,25(OH)(2) D(3) ) treatment (2.56 nmol/kg i.p. daily×4) increased PepT1, Mrp2, Mrp4, Asbt, but not Mdr1/P-gp in the rat small intestine. In this study, the intestinal everted sac technique, together with various select probes: mannitol (paracellular transport), glycylsarcosine (PepT1), 5(and 6)-carboxy-2',7'-dichlorofluorescein (CDF) diacetate (precursor of CDF for Mrp2), adefovir dipivoxil (precursor of adefovir for Mrp4) and digoxin (P-gp) was used to examine the functional changes of these transporters. After establishing identical permeabilities (P(app) ) of mannitol for the apical-to-basolateral (A-to-B) and basolateral-to-apical (B-to-A) directions at 20 min in 1,25(OH)(2) D(3) -treated vs. vehicle-treated duodenal, jejunal and ileal everted sacs, a significant enhancement of net A-to-B transport of glycylsarcosine in the duodenum, increased B-to-A transport of CDF and A-to-B and B-to-A transport of adefovir in the jejunum were observed with 1,25(OH)(2) D(3) treatment. However, the A-to-B and B-to-A transport of digoxin in the ileum was unchanged. These changes in transporter function in the rat intestinal everted sac corresponded well to changes in proteins that were observed previously. This study confirms that the rat intestinal PepT1, Mrp2 and Mrp4, but not P-gp are functionally induced by 1,25(OH)(2) D(3) treatment via the vitamin D receptor (VDR). Copyright © 2011 John Wiley & Sons, Ltd. |
| | |
Authors:
|
Han-Joo Maeng; Matthew R Durk; Edwin C Y Chow; Ragia Ghoneim; K Sandy Pang |
Publication Detail:
|
Type: Journal Article Date: 2011-01-14 |
Journal Detail:
|
Title: Biopharmaceutics & drug disposition Volume: 32 ISSN: 1099-081X ISO Abbreviation: Biopharm Drug Dispos Publication Date: 2011 Mar |
Date Detail:
|
Created Date: 2011-02-22 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7911226 Medline TA: Biopharm Drug Dispos Country: England |
Other Details:
|
Languages: eng Pagination: 112-25 Citation Subset: IM |
Copyright Information:
|
Copyright © 2011 John Wiley & Sons, Ltd. |
Affiliation:
|
Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, Ontario, Canada M5S 3M2. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Effects of formulation and route of administration on the systemic availability of Ex-RAD(®) , a ne...
Next Document: Hepatic fibrosis does not affect the pharmacokinetics of 5-fluorouracil in rats.