Document Detail


1,2,3,4,6-penta-O-galloyl-beta-D-glucose protects splenocytes against radiation-induced apoptosis in murine splenocytes.
MedLine Citation:
PMID:  20606300     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Antioxidant property and hematopoietic repair capacity are important characteristics of radioprotective agents. Some studies have demonstrated that 1,2,3,4,6-penta-O-galloyl-beta-D-glucose (PGG), a molecule isolated from the waterlily, has antioxidant, hematopoietic repair, and anti-inflammatory activities. In this study, we try to determine whether PGG extracted from a lily, Nymphaea tetragona var. angusta, has radioprotective effects on splenocytes in vitro against (60)Co gamma-ray irradiation with absorption doses of 2 Gy and 4 Gy. Results show that PGG treatment dramatically enhances the proliferation of splenocytes compared with irradiated but untreated controls. In addition, PGG treatment before irradiation protects the splenocytes from lethal effects of irradiation and decreases DNA damages as identified by the alkaline comet assay. PGG-treated cells also show less radiation-induced apoptosis. These cells have lower concentrations of the pro-apoptotic protein p53 and more of the anti-apoptotic protein Bcl-2. The results presented in this study suggest that PGG has a cytoprotective effect on immune cells exposed to normally damaging amount of radiation. Thus, PGG could be an effective, non-toxic radioprotective agent.
Authors:
So Jin Bing; Min Ju Kim; Eunjin Park; Ginnae Ahn; Dae Seung Kim; Ryeo Kyeong Ko; Nam Ho Lee; Taekyun Shin; Jae Woo Park; Youngheun Jee
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biological & pharmaceutical bulletin     Volume:  33     ISSN:  1347-5215     ISO Abbreviation:  Biol. Pharm. Bull.     Publication Date:  2010  
Date Detail:
Created Date:  2010-07-07     Completed Date:  2010-11-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9311984     Medline TA:  Biol Pharm Bull     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1122-7     Citation Subset:  IM    
Affiliation:
Department of Veterinary Medicine and Applied Radiological Science Research Institute, Jeju National University, Jeju 690-756, South Korea.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / radiation effects*
Blotting, Western
Cell Proliferation / drug effects
Cells, Cultured
Comet Assay
Hydrolyzable Tannins / pharmacology*
Mice
Reactive Oxygen Species / metabolism
Spleen / cytology,  drug effects*,  metabolism,  radiation effects
Chemical
Reg. No./Substance:
0/Hydrolyzable Tannins; 0/Reactive Oxygen Species; 14937-32-7/beta-penta-O-galloyl-glucose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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