Document Detail


1,2-propanediol and the type of cryopreservation procedure adversely affect mouse oocyte physiology.
MedLine Citation:
PMID:  16905767     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The aim of this work was to examine the effect of 1,2-propanediol (PrOH) and type of cryopreservation procedure (slow freezing and vitrification) on oocyte physiology. METHODS: Intracellular calcium of mouse metaphase II (MII) oocytes was quantified by fluorescence microscopy. The effect of PrOH on cell physiology was further assessed through analysis of zona pellucida hardening and cellular integrity. Protein profiles of cryopreserved oocytes were generated by time-of-flight mass spectrometry (TOF-MS). RESULTS: PrOH caused a protracted increase in calcium, which was sufficient to induce zona pellucida hardening and cellular degeneration. Using 'nominally calcium free' media during PrOH exposure significantly reduced the detrimental effects. Proteomic analysis identified numerous up- and down-regulated proteins after slow freezing when compared with control and vitrified oocytes. CONCLUSIONS: Using such approaches to assess effects on cellular physiology is fundamental to improving assisted reproduction techniques (ART). This study demonstrates that PrOH causes a significant rise in intracellular calcium. Using calcium-free media significantly reduced the increase in calcium and the associated detrimental physiological effects, suggesting that calcium-free media should be used with PrOH. In addition, analysis of the oocyte proteome following cryopreservation revealed that slow freezing has a significant effect on protein expression. In contrast, vitrification had a minimal impact, indicating that it has a fundamental advantage for the cryopreservation of oocytes.
Authors:
M G Larman; M G Katz-Jaffe; C B Sheehan; D K Gardner
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-08-12
Journal Detail:
Title:  Human reproduction (Oxford, England)     Volume:  22     ISSN:  0268-1161     ISO Abbreviation:  Hum. Reprod.     Publication Date:  2007 Jan 
Date Detail:
Created Date:  2006-12-12     Completed Date:  2007-03-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8701199     Medline TA:  Hum Reprod     Country:  England    
Other Details:
Languages:  eng     Pagination:  250-9     Citation Subset:  IM    
Affiliation:
Colorado Center for Reproductive Medicine, Englewood, CO 80133, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Calcium / metabolism,  pharmacology
Calcium Signaling / drug effects
Cryopreservation / methods*
Female
Mice
Oocytes / drug effects,  physiology*
Propylene Glycol / pharmacology*
Proteome / drug effects
Zona Pellucida / drug effects,  physiology
Chemical
Reg. No./Substance:
0/Proteome; 57-55-6/Propylene Glycol; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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