| 1,2-bis(2-aminophenoxy)ethane-N,N,N'N'-tetraacetic acid (BAPTA-AM) inhibits caffeine-induced apoptosis in human neuroblastoma cells. | |
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MedLine Citation:
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PMID: 15039113 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In the present study, it was investigated whether 1,2-bis(2-aminophenoxy)ethane-N,N,N'N'-tetraacetic acid (BAPTA-AM), an intracellular Ca(2+) chelator, possesses protective effect against caffeine-induced apoptosis in the central nervous system. Through morphological and biochemical analyses, cells treated with caffeine exhibited several apoptotic features. On the other hand, cells treated with caffeine and BAPTA-AM, showed decreased occurrence of apoptotic features. In addition, it was shown that BAPTA-AM treatment inhibits caffeine-induced increase of caspase-3 enzyme activity. These results show that caffeine induces apoptotic death in human SK-N-MC neuroblastoma cells and BAPTA-AM prevents apoptosis by attenuating caffeine-induced caspase-3 activation. |
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Authors:
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Mi-Hyeon Jang; Min-Chul Shin; Young-Wuk Cho; Hyung-Hwan Baik; Sung-Soo Kim; Eun-Gu Hwang; Chang-Ju Kim |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Neuroscience letters Volume: 358 ISSN: 0304-3940 ISO Abbreviation: Neurosci. Lett. Publication Date: 2004 Apr |
Date Detail:
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Created Date: 2004-03-24 Completed Date: 2004-07-16 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7600130 Medline TA: Neurosci Lett Country: Ireland |
Other Details:
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Languages: eng Pagination: 189-92 Citation Subset: IM |
Affiliation:
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Department of Physiology, College of Medicine, Kyung Hee University, #1 Hoigi-dong, Dongdaemoon-gu, Seoul 130-701, South Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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drug effects*,
physiology Caffeine / antagonists & inhibitors*, toxicity Caspase 3 Caspases / drug effects*, metabolism Cell Line, Tumor Cell Survival / drug effects, physiology Central Nervous System / drug effects*, metabolism Chelating Agents / pharmacology DNA Fragmentation / drug effects, physiology Dose-Response Relationship, Drug Egtazic Acid / analogs & derivatives*, pharmacology* Humans Neuroblastoma / drug therapy, metabolism, pathology Neurons / drug effects*, metabolism, pathology |
| Chemical | |
Reg. No./Substance:
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0/Chelating Agents; 139890-68-9/1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid acetoxymethyl ester; 58-08-2/Caffeine; 67-42-5/Egtazic Acid; EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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