Document Detail


12-O-tetradecanoylphorbol-13-acetate induces selective desquamation of superficial cells in rat urinary bladder epithelium.
MedLine Citation:
PMID:  1617698     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
12-O-tetradecanoylphorbol-13-acetate (TPA) is known to affect the proliferation and/or differentiation of several types of cells. We injected TPA directly into the lumen of rat bladder to determine, using scanning and transmission electron microscopy, its effects on the bladder epithelium in vivo. At 1 h after TPA injection (1 microgram/ml), the superficial cells of the epithelium had changed their morphology, and large spherical vacuoles occupied their cytoplasm. In some areas, the underlying intermediate cells were exposed by the desquamation of the superficial cells. During the next few hours, TPA was excreted from the bladder lumen by voluntary micturition, but the desquamation of the superficial cells proceeded further. All the superficial cells were lost from the luminal surface by 24 h after TPA injection. The changes noted were specific for the superficial cells and were not observed in the intermediate or basal cells. After 24 h, part of the epithelium had a three-layer structure, indicating that regeneration was taking place. These results demonstrate that TPA selectively affects and desquamates superficial cells in a short period of time. This experimental system may be useful for studying in vivo cell proliferation and/or differentiation.
Authors:
S Watanabe; J Sasaki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell and tissue research     Volume:  268     ISSN:  0302-766X     ISO Abbreviation:  Cell Tissue Res.     Publication Date:  1992 May 
Date Detail:
Created Date:  1992-08-03     Completed Date:  1992-08-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0417625     Medline TA:  Cell Tissue Res     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  239-45     Citation Subset:  IM    
Affiliation:
Department of Anatomy, Okayama University Medical School, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Epithelium / drug effects,  ultrastructure
Female
Rats
Rats, Inbred Strains
Tetradecanoylphorbol Acetate / pharmacology*
Urinary Bladder / drug effects*,  pathology
Vacuoles
Chemical
Reg. No./Substance:
16561-29-8/Tetradecanoylphorbol Acetate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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