Document Detail

12-O-tetradecanoylphorbol-13-acetate induces selective desquamation of superficial cells in rat urinary bladder epithelium.
MedLine Citation:
PMID:  1617698     Owner:  NLM     Status:  MEDLINE    
12-O-tetradecanoylphorbol-13-acetate (TPA) is known to affect the proliferation and/or differentiation of several types of cells. We injected TPA directly into the lumen of rat bladder to determine, using scanning and transmission electron microscopy, its effects on the bladder epithelium in vivo. At 1 h after TPA injection (1 microgram/ml), the superficial cells of the epithelium had changed their morphology, and large spherical vacuoles occupied their cytoplasm. In some areas, the underlying intermediate cells were exposed by the desquamation of the superficial cells. During the next few hours, TPA was excreted from the bladder lumen by voluntary micturition, but the desquamation of the superficial cells proceeded further. All the superficial cells were lost from the luminal surface by 24 h after TPA injection. The changes noted were specific for the superficial cells and were not observed in the intermediate or basal cells. After 24 h, part of the epithelium had a three-layer structure, indicating that regeneration was taking place. These results demonstrate that TPA selectively affects and desquamates superficial cells in a short period of time. This experimental system may be useful for studying in vivo cell proliferation and/or differentiation.
S Watanabe; J Sasaki
Related Documents :
6300468 - Activation of latent epstein-barr virus genomes: selective stimulation of synthesis of ...
10642518 - Neuroserpin is expressed in the pituitary and adrenal glands and induces the extension ...
11078338 - The human neuroblastoma sk-sy5y cell line bears functional endothelin-a-receptors and e...
6274878 - The relationship between tumor promoter binding and epstein-barr virus induction in hum...
561788 - Homogeneous cell populations from rabbit kidney cortex. proximal, distal tubule, and re...
18504428 - Activation of the cyclin d2 and cdk6 genes through nf-kappab is critical for cell-cycle...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell and tissue research     Volume:  268     ISSN:  0302-766X     ISO Abbreviation:  Cell Tissue Res.     Publication Date:  1992 May 
Date Detail:
Created Date:  1992-08-03     Completed Date:  1992-08-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0417625     Medline TA:  Cell Tissue Res     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  239-45     Citation Subset:  IM    
Department of Anatomy, Okayama University Medical School, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Epithelium / drug effects,  ultrastructure
Rats, Inbred Strains
Tetradecanoylphorbol Acetate / pharmacology*
Urinary Bladder / drug effects*,  pathology
Reg. No./Substance:
16561-29-8/Tetradecanoylphorbol Acetate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Ultrastructure of the basement membrane and its precursor in developing rat submandibular gland as s...
Next Document:  Structural changes in condylar cartilage following prolonged exposure to the human parathyroid hormo...