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12-Methyltetradecanoic Acid, a Branched-Chain Fatty Acid, Represses the Extracellular Production of Surfactants Required for Swarming Motility in Pseudomonas aeruginosa PAO1.
MedLine Citation:
PMID:  22446119     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Pseudomonas aeruginosa is known to produce surfactants that are involved in its swarming motility behavior, such as rhamnolipids and their precursors-3-(3-hydroxyalkanoyloxy)-alkanoic acids (HAAs). In P. aeruginosa PAO1, swarming motility is inhibited by some fatty acids, including branched-chain fatty acids and unsaturated fatty acids. In the present study, addition of 12-methyltetradecanoic acid (12-MTA, anteiso-C15:0) to an agar medium markedly repressed surfactant activity in the extracellular fraction of a P. aeruginosa culture in a drop collapse assay. Further, an extracellular fraction of a culture of rhlA mutant P. aeruginosa, which did not produce both rhamnolipids and HAAs, showed a complete loss of surfactant activity and markedly reduced swarming activity. In contrast, an extracellular fraction of a culture of rhlB mutant P. aeruginosa, which produced HAAs but not rhamnolipids, showed moderate swarming activity and weak extracellular surfactant activity that was lost on the addition of 12-MTA to the agar medium. Expression of the rhlAB operon from the plasmid pMR2 restored normal swarming motility on 12-MTA-containing agar medium. Taken together, these findings indicate that 12-MTA reduced extracellular surfactant activity, thus resulting in a swarming defect in P. aeruginosa PAO1.
Authors:
Tetsuyoshi Inoue; Teruo Kuroda; Naoya Ohara
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Japanese journal of infectious diseases     Volume:  65     ISSN:  1344-6304     ISO Abbreviation:  Jpn. J. Infect. Dis.     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-03-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100893704     Medline TA:  Jpn J Infect Dis     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  126-31     Citation Subset:  IM    
Affiliation:
Department of Oral Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama 700-8525, Japan.
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