| 10t,12c-conjugated linoleic acid inhibits lipopolysaccharide-induced cyclooxygenase expression in vitro and in vivo. | |
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MedLine Citation:
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PMID: 16061956 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Previous data demonstrated that conjugated linoleic acid (CLA) reduced eicosanoid release from select organs. We hypothesized that one active CLA isomer was responsible for the reduced prostaglandin release and that the mechanism was through the inhibition of inducible cyclooxygenase-2 (COX-2). Here, we examined the effects of 10t,12c-CLA and 9c,11t-CLA on COX-2 protein/mRNA expression, prostaglandin E(2) (PGE(2)) production, and the mechanism by which CLA affects COX-2 expression and prostaglandin release. The COX-2 protein expression level was inhibited 80% by 10t, 12c-CLA and 26% by 9c,11t-CLA at 100 microM in vitro. PGE(2) production was decreased from 5.39 to 1.12 ng/2 x 10(6) cells by 10t,12c-CLA and from 5.7 to 4.5 ng/2 x 10(6) cells by 9c,11t-CLA at 100 microM. Mice fed 10t,12c-CLA but not 9c,11t-CLA were found to have a 34% decrease in COX-2 protein and a 43% reduction of PGE(2) release in the lung. 10t,12c-CLA reduced COX-2 mRNA expression level by 30% at 100 microM in vitro and by 30% in mouse lung in vivo. Reduced COX-2 mRNA was attributable to an inhibition of the nuclear factor kappaB (NF-kappaB) pathway by 10t,12c-CLA. These data suggested that the inhibition of NF-kappaB was one of the mechanisms for the reduced COX-2 expression and PGE(2) release by 10t,12c-CLA. |
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Authors:
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Guangming Li; David Barnes; Daniel Butz; Dale Bjorling; Mark E Cook |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2005-08-01 |
Journal Detail:
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Title: Journal of lipid research Volume: 46 ISSN: 0022-2275 ISO Abbreviation: J. Lipid Res. Publication Date: 2005 Oct |
Date Detail:
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Created Date: 2005-09-19 Completed Date: 2006-01-12 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: United States |
Other Details:
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Languages: eng Pagination: 2134-42 Citation Subset: IM |
Affiliation:
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Molecular and Environmental Toxicology, University of Wisconsin-Madison, Madison, WI 53706, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line Cyclooxygenase 2 Inhibitors / pharmacology* Dinoprostone / antagonists & inhibitors, biosynthesis I-kappa B Proteins / drug effects Isomerism Linoleic Acid / pharmacology Linoleic Acids, Conjugated / pharmacology* Lipopolysaccharides / pharmacology Male Mice Mice, Inbred BALB C Phosphorylation / drug effects RNA, Messenger / drug effects* Signal Transduction / drug effects |
| Chemical | |
Reg. No./Substance:
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0/Cyclooxygenase 2 Inhibitors; 0/I-kappa B Proteins; 0/Linoleic Acids, Conjugated; 0/Lipopolysaccharides; 0/RNA, Messenger; 139874-52-5/NF-kappaB inhibitor alpha; 2197-37-7/Linoleic Acid; 363-24-6/Dinoprostone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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