Document Detail


1064 nm Deep Near-Infrared (NIR) Excited Raman Microspectroscopy for Studying Photolabile Organisms.
MedLine Citation:
PMID:  21513590     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
We have constructed a 1064 nm deep near-infrared (NIR) excited multichannel Raman microspectrometer using an InP/InGaAsP multichannel detector. This microspectrometer achieves high sensitivity suitable for in vivo measurements of single living cells with lateral resolution of 0.7 μm and depth resolution of 3.1 μm. It has been applied to the structural analysis of living cyanobacterial cells, well-known model organisms for photosynthesis research, which are too photolabile to be measured with visible laser excitation. High signal-to-noise ratio (S/N) Raman spectra have been obtained from carotenoid, chlorophyll α, and phycocyanin in a single living cyanobacterial cell with no appreciable interference from autofluorescence or photodamage. Sub-micrometer mapping of Raman intensities provides clear distribution images of the three pigments inside the cell.
Authors:
Masahiro Ando; Miwa Sugiura; Hidenori Hayashi; Hiro-O Hamaguchi
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied spectroscopy     Volume:  65     ISSN:  1943-3530     ISO Abbreviation:  Appl Spectrosc     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-04-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372406     Medline TA:  Appl Spectrosc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  488-92     Citation Subset:  IM    
Affiliation:
Department of Chemistry, School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
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