Document Detail

1-Palmitoyl-2-thiopalmitoyl phosphatidylcholine, a highly specific chromogenic substrate of phospholipase A2.
MedLine Citation:
PMID:  3124835     Owner:  NLM     Status:  MEDLINE    
1-Palmitoyl-2-thiopalmitoyl phosphatidylcholine (2-thioPC), a structurally modified phospholipid analog is specifically hydrolyzed by phospholipase A2 to liberate 2-thiolysophosphatidylcholine and palmitic acid. The sulfhydryl group of the product is readily trapped by 5,5'-dithiobis (2-nitrobenzoic acid) allowing continuous spectrophotometric monitoring of the enzymatic reaction. The rates of hydrolysis by bee-venom phospholipase A2 have been determined in a series of Triton X-100 containing mixed micelles. At 1 mM 2-thioPC increasing the concentration of Triton X-100 from 4 to 16 mM changes the specific activity of bee-venom phospholipase A2 from 96.9 to 17.9 mumol/min/mg, about one order of magnitude lower than dipalmitoyl phosphatidylcholine hydrolysis in micelles of similar composition. The chromogenic substrate is the first phospholipid analog exhibiting absolute specificity for phospholipase A2 and should be applicable to spectrophotometric detection and kinetic characterization of both water soluble and membrane-bound forms.
C Balet; K A Clingman; J Hajdu
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  150     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1988 Jan 
Date Detail:
Created Date:  1988-03-09     Completed Date:  1988-03-09     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  561-7     Citation Subset:  IM    
Department of Chemistry, California State University, Northridge 91330.
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MeSH Terms
Bacillus cereus / enzymology
Bee Venoms
Pancreas / enzymology
Phosphatidylcholines / metabolism*
Phospholipases / metabolism*
Phospholipases A / metabolism*
Phospholipases A2
Type C Phospholipases / metabolism
Grant Support
Reg. No./Substance:
0/Bee Venoms; 0/Micelles; 0/Phosphatidylcholines; 113881-60-0/1-palmitoyl-2-thiopalmitoyl phosphatidylcholine; EC 3.1.-/Phospholipases; EC 3.1.1.-/Phospholipases A; EC A2; EC 3.1.4.-/Type C Phospholipases

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