| 1-Palmitoyl-2-thiopalmitoyl phosphatidylcholine, a highly specific chromogenic substrate of phospholipase A2. | |
| | |
MedLine Citation:
|
PMID: 3124835 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
1-Palmitoyl-2-thiopalmitoyl phosphatidylcholine (2-thioPC), a structurally modified phospholipid analog is specifically hydrolyzed by phospholipase A2 to liberate 2-thiolysophosphatidylcholine and palmitic acid. The sulfhydryl group of the product is readily trapped by 5,5'-dithiobis (2-nitrobenzoic acid) allowing continuous spectrophotometric monitoring of the enzymatic reaction. The rates of hydrolysis by bee-venom phospholipase A2 have been determined in a series of Triton X-100 containing mixed micelles. At 1 mM 2-thioPC increasing the concentration of Triton X-100 from 4 to 16 mM changes the specific activity of bee-venom phospholipase A2 from 96.9 to 17.9 mumol/min/mg, about one order of magnitude lower than dipalmitoyl phosphatidylcholine hydrolysis in micelles of similar composition. The chromogenic substrate is the first phospholipid analog exhibiting absolute specificity for phospholipase A2 and should be applicable to spectrophotometric detection and kinetic characterization of both water soluble and membrane-bound forms. |
| | |
Authors:
|
C Balet; K A Clingman; J Hajdu |
Related Documents
:
|
19466285 - Modulation of peritoneal macrophage activity by the saturation state of the fatty acid ... 6679725 - Molecular species of diacylphosphatidylethanolamine in rat and mouse heart given the sa... 4026805 - Sidedness studies of thylakoid phosphatidylglycerol in higher plants. 8549755 - Long-term potentiation requires activation of calcium-independent phospholipase a2. 9633995 - Effects of iodotyrosines, thyronines, iodothyroacetic acids and thyromimetic analogues ... 11903735 - Helicobacter pylori alters n-6 fatty acid metabolism and prostaglandin e2 synthesis in ... |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Biochemical and biophysical research communications Volume: 150 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1988 Jan |
Date Detail:
|
Created Date: 1988-03-09 Completed Date: 1988-03-09 Revised Date: 2007-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 561-7 Citation Subset: IM |
Affiliation:
|
Department of Chemistry, California State University, Northridge 91330. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Bacillus cereus / enzymology Bee Venoms Kinetics Micelles Pancreas / enzymology Phosphatidylcholines / metabolism* Phospholipases / metabolism* Phospholipases A / metabolism* Phospholipases A2 Swine Type C Phospholipases / metabolism |
| Grant Support | |
ID/Acronym/Agency:
|
DK 36215/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Bee Venoms; 0/Micelles; 0/Phosphatidylcholines; 113881-60-0/1-palmitoyl-2-thiopalmitoyl phosphatidylcholine; EC 3.1.-/Phospholipases; EC 3.1.1.-/Phospholipases A; EC 3.1.1.4/Phospholipases A2; EC 3.1.4.-/Type C Phospholipases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Oxidative metabolism of cultured adult cardiomyocytes.
Next Document: Stimulation of DNA synthesis in murine lymphocytes by the drug swainsonine: immunomodulatory propert...