| 1-methylthio-D-xylulose 5-phosphate methylsulfurylase: a novel route to 1-deoxy-D-xylulose 5-phosphate in Rhodospirillum rubrum. | |
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MedLine Citation:
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PMID: 23035785 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Rhodospirillum rubrum produces 5-methylthioadenosine (MTA) from S-adenosylmethionine in polyamine biosynthesis; however, R. rubrum lacks the classical methionine salvage pathway. Instead, MTA is converted to 5-methylthio-d-ribose 1-phosphate (MTR 1-P) and adenine; MTR 1-P is isomerized to 1-methylthio-d-xylulose 5-phosphate (MTXu 5-P) and reductively dethiomethylated to 1-deoxy-d-xylulose 5-phosphate (DXP), an intermediate in the nonmevalonate isoprenoid pathway [Erb, T. J., et al. (2012) Nat. Chem. Biol., in press]. Dethiomethylation, a novel route to DXP, is catalyzed by MTXu 5-P methylsulfurylase. An active site Cys displaces the enolate of DXP from MTXu 5-P, generating a methyl disulfide intermediate. |
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Authors:
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Benjamin P Warlick; Bradley S Evans; Tobias J Erb; Udipi A Ramagopal; Jaya Sriram; Heidi J Imker; J Michael Sauder; Jeffrey B Bonanno; Stephen K Burley; F Robert Tabita; Steven C Almo; Jonathan S Sweedler; John A Gerlt |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2012-10-08 |
Journal Detail:
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Title: Biochemistry Volume: 51 ISSN: 1520-4995 ISO Abbreviation: Biochemistry Publication Date: 2012 Oct |
Date Detail:
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Created Date: 2012-10-23 Completed Date: 2013-01-07 Revised Date: 2013-04-22 |
Medline Journal Info:
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Nlm Unique ID: 0370623 Medline TA: Biochemistry Country: United States |
Other Details:
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Languages: eng Pagination: 8324-6 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Metabolic Networks and Pathways Nuclear Magnetic Resonance, Biomolecular Pentosephosphates / biosynthesis*, metabolism Rhodospirillum rubrum / metabolism* Ribosemonophosphates / metabolism Sulfurtransferases / metabolism* Thioglycosides / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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P01 GM071790/GM/NIGMS NIH HHS; P01GM071790/GM/NIGMS NIH HHS; R01 GM065155/GM/NIGMS NIH HHS; R01 GM095742/GM/NIGMS NIH HHS; R01GM065166/GM/NIGMS NIH HHS; R01GM095742/GM/NIGMS NIH HHS; U54 GM093342/GM/NIGMS NIH HHS; U54GM074945/GM/NIGMS NIH HHS; U54GM093342/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/1-deoxylulose 5-phosphate; 0/5'-methylthioribose 1-phosphate; 0/Pentosephosphates; 0/Ribosemonophosphates; 0/Thioglycosides; 60802-29-1/xylulose-5-phosphate; EC 2.8.1.-/1-methylthio-D-xylulose 5-phosphate methylsulfurylase, Rhodospirillum rubrum; EC 2.8.1.-/Sulfurtransferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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