Document Detail


1-methylthio-D-xylulose 5-phosphate methylsulfurylase: a novel route to 1-deoxy-D-xylulose 5-phosphate in Rhodospirillum rubrum.
MedLine Citation:
PMID:  23035785     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Rhodospirillum rubrum produces 5-methylthioadenosine (MTA) from S-adenosylmethionine in polyamine biosynthesis; however, R. rubrum lacks the classical methionine salvage pathway. Instead, MTA is converted to 5-methylthio-d-ribose 1-phosphate (MTR 1-P) and adenine; MTR 1-P is isomerized to 1-methylthio-d-xylulose 5-phosphate (MTXu 5-P) and reductively dethiomethylated to 1-deoxy-d-xylulose 5-phosphate (DXP), an intermediate in the nonmevalonate isoprenoid pathway [Erb, T. J., et al. (2012) Nat. Chem. Biol., in press]. Dethiomethylation, a novel route to DXP, is catalyzed by MTXu 5-P methylsulfurylase. An active site Cys displaces the enolate of DXP from MTXu 5-P, generating a methyl disulfide intermediate.
Authors:
Benjamin P Warlick; Bradley S Evans; Tobias J Erb; Udipi A Ramagopal; Jaya Sriram; Heidi J Imker; J Michael Sauder; Jeffrey B Bonanno; Stephen K Burley; F Robert Tabita; Steven C Almo; Jonathan S Sweedler; John A Gerlt
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-10-08
Journal Detail:
Title:  Biochemistry     Volume:  51     ISSN:  1520-4995     ISO Abbreviation:  Biochemistry     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-23     Completed Date:  2013-01-07     Revised Date:  2013-10-30    
Medline Journal Info:
Nlm Unique ID:  0370623     Medline TA:  Biochemistry     Country:  United States    
Other Details:
Languages:  eng     Pagination:  8324-6     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
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MeSH Terms
Descriptor/Qualifier:
Metabolic Networks and Pathways
Nuclear Magnetic Resonance, Biomolecular
Pentosephosphates / biosynthesis*,  metabolism
Rhodospirillum rubrum / metabolism*
Ribosemonophosphates / metabolism
Sulfurtransferases / metabolism*
Thioglycosides / metabolism
Grant Support
ID/Acronym/Agency:
P01 GM071790/GM/NIGMS NIH HHS; P01GM071790/GM/NIGMS NIH HHS; R01 GM065155/GM/NIGMS NIH HHS; R01 GM095742/GM/NIGMS NIH HHS; R01GM065166/GM/NIGMS NIH HHS; R01GM095742/GM/NIGMS NIH HHS; U54 GM074945/GM/NIGMS NIH HHS; U54 GM093342/GM/NIGMS NIH HHS; U54GM074945/GM/NIGMS NIH HHS; U54GM093342/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/1-deoxylulose 5-phosphate; 0/5'-methylthioribose 1-phosphate; 0/Pentosephosphates; 0/Ribosemonophosphates; 0/Thioglycosides; 60802-29-1/xylulose-5-phosphate; EC 2.8.1.-/1-methylthio-D-xylulose 5-phosphate methylsulfurylase, Rhodospirillum rubrum; EC 2.8.1.-/Sulfurtransferases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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