Document Detail

1-Methylguanosine in place of Y base at position 37 in phenylalanine tRNA is responsible for its shiftiness in retroviral ribosomal frameshifting.
MedLine Citation:
PMID:  11145896     Owner:  NLM     Status:  MEDLINE    
Many mammalian retroviruses express their protease and polymerase by ribosomal frameshifting. It was originally proposed that a specialized shifty tRNA promotes the frameshift event. We previously observed that phenylalanine tRNA(Phe) lacking the highly modified wybutoxosine (Y) base on the 3' side of its anticodon stimulated frameshifting, demonstrating that this tRNA is shifty. We now report the shifty tRNA(Phe) contains 1-methylguanosine (m(1)G) in place of Y and that the m(1)G form from rabbit reticulocytes stimulates frameshifting more efficiently than its m(1)G-containing counterpart from mouse neuroblastoma cells. The latter tRNA contains unmodified C and G nucleosides at positions 32 and 34, respectively, while the former tRNA contains the analogous 2'-O-methylated nucleosides at these positions. The data suggest that not only does the loss of a highly modified base from the 3' side of the anticodon render tRNA(Phe) shifty, but the modification status of the entire anticodon loop contributes to the degree of shiftiness. Possible biological consequences of these findings are discussed.
B A Carlson; J F Mushinski; D W Henderson; S Y Kwon; P F Crain; B J Lee; D L Hatfield
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Virology     Volume:  279     ISSN:  0042-6822     ISO Abbreviation:  Virology     Publication Date:  2001 Jan 
Date Detail:
Created Date:  2001-01-26     Completed Date:  2001-03-01     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  130-5     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Academic Press.
Section on the Molecular Biology of Selenium, National Cancer Institute, Bethesda, Maryland, 20892, USA.
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MeSH Terms
Base Sequence
Frameshifting, Ribosomal*
Guanine / analogs & derivatives*,  chemistry*
Guanosine / analogs & derivatives*,  chemistry*
Liver / chemistry
Molecular Sequence Data
Neuroblastoma / chemistry
Nucleic Acid Conformation
RNA, Transfer, Phe / chemistry*,  genetics*,  metabolism
Reticulocytes / chemistry
Retroviridae / genetics*
Grant Support
Reg. No./Substance:
0/8-methylguanosine; 0/RNA, Transfer, Phe; 118-00-3/Guanosine; 33359-03-4/wye; 73-40-5/Guanine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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