PURPOSE: Analysis of non-uveitic entities that were referred as posterior uveitis to a uveitis referral center. METHODS: We came across and analyzed 32 cases of non-uveitic entities (1.12%) that had been misdiagnosed and referred as posterior uveitis out of 2846 outpatient cases of posterior uveitis registered from 1990 to June ...

PURPOSE: A case of atopic keratoconjunctivitis complicated by bilateral infectious ulcers is described. METHODS: Both eyes underwent complete ophthalmologic examination and in vivo confocal microscopy imaging by using the Heidelberg Retina Tomograph II Rostock-cornea-module (HRT II-RCM) and were photographed. RESULTS: Topical antibiotics and corticosteroids associated with oral antibiotics and corticosteroids ...

We present three-dimensional fluorescence yield tomography of a tissue phantom in a noncontact reflectance imaging setup. The method employs planar illumination with modulated light and frequency domain fluorescence measurements made on the illumination plane. An adaptive finite-element algorithm is used to handle the ill-posed and computationally demanding inverse image reconstruction ...

As a critical dimension shrinks, the degradation in image quality caused by wavefront aberrations of projection optics in lithographic tools becomes a serious problem. It is necessary to establish a technique for a fast and accurate in situ aberration measurement. We introduce what we believe to be a novel technique ...

Biomechanical elastic properties are among the many variables used to characterize in vivo and in vitro tissues. Since these properties depend largely on the micro- and macroscopic structural organization tissue, it is crucial to understand the mechanical properties and the alterations that occur tissues respond to external forces or to ...

A technique for permanently capturing a replica impression of biological cells has been developed to facilitate analysis using nanometer resolution imaging tools, namely the atomic force microscope (AFM). The method, termed Bioimprint, creates a permanent cell 'footprint' in a non-biohazardous Poly (dimethylsiloxane) (PDMS) polymer composite. The transfer of nanometer scale ...

Optical coherence tomography (OCT) poor mapping resolution has been pointed out as the biggest disadvantage of this technique when compared to others, e.g., retinal thickness analyzer. In this work we were able to solve this problem by developing an atlas of macular thickness of the human retina into which OCT ...

To investigate the capabilities of ultrahigh-resolution optical coherence tomography (UHR OCT); to compare with the commercially available OCT standard-resolution system, StratusOCT, for imaging of idiopathic juxtafoveal retinal telangiectasis (IJT); and to demonstrate that UHR OCT provides additional information on disease morphology, pathogenesis, and management. Retrospective, observational, interventional case series. Nineteen ...

BACKGROUND: Subthreshold laser therapy has been shown to cause drusen reduction. Using this method, visible laser burns are not created in the retina; presumably, the energy is absorbed by the retinal pigment epithelium (RPE) and stimulates reabsorption of drusen material, sparing photoreceptors from destruction. We hypothesized that autofluorescence (AF) changes ...

Mitochondria are now known to function physiologically not only in the production of ATP as the major cellular energy source, but also in the regulation of intracellular signaling, in, for example, stress-induced apoptosis and buffering of cytosolic calcium. It should be noted, when interpreting mitochondrial studies in situ, that mitochondria ...

PURPOSE: To evaluate the time course and morphologic features of verteporfin therapy-induced vascular effects using 3-dimensional topographic angiography (TAG) in patients with choroidal neovascularization (CNV). DESIGN: Prospective observational case series. PARTICIPANTS: Fifty-three eyes of 53 patients with neovascular age-related macular degeneration and Treatment of Age-Related Macular Degeneration with Photodynamic Therapy/Verteporfin ...

PURPOSE: To investigate the fundus autofluorescence (FAF) imaging findings in patients with serous and drusenoid pigment epithelial detachment (PED) associated with age-related macular degeneration (AMD). DESIGN: Observational case series. METHODS: Fourteen eyes of 13 consecutive patients with serous (n = 10) and drusenoid (n = 4) PED were prospectively included. ...

BACKGROUND: Optical coherence tomography (OCT) permits high-resolution, real-time, infrared-generated imaging of tissue microstructures by a probe inserted through the endoscope operative channel. Resolution is approximately 10 microm and the penetration depth of the near-focus probe is about 1 mm. The probe can be inserted into the main pancreatic duct (MPD) ...

PURPOSE: We describe the technique of fluorescence image guided optical coherence tomography (FG-OCT). We examined its ability to enhance specificity and sensitivity for the noninvasive diagnosis of early bladder cancer. MATERIALS AND METHODS: Transitional cell carcinoma was developed in 54 Fisher 344 female rats by intravesical methyl-nitroso-urea instillations. Two or ...

We describe the clinical presentation of a choroidal amelanotic melanoma in a 46-year-old woman with oculocutaneous albinism. Clinical aspects, investigations, and management are presented, and findings from computed tomography and magnetic resonance imaging are described. Microscopic findings and histopathological features, demonstrating a spindle B-cell melanoma of the choroid, are also ...

After much effort in surface chemistry development and optimization by several groups, fluorescent semiconductor nanocrystals probes, also known as quantum dots or qdots, are now entering the realm of biological applications with much to offer to biologists. The road to success has been paved with hurdles but from these efforts ...

PURPOSE: To evaluate the diagnostic ability of third-generation optical coherence tomography (Stratus OCT) with a normative database to detect localized retinal nerve fiber layer (RNFL) defects. DESIGN: Cross-sectional study. PARTICIPANTS: One eye of each of 55 subjects (43 normal-tension glaucoma and 12 primary open-angle glaucoma subjects) with localized, wedge-shaped RNFL ...

Semiconductor quantum dots (QDs) have emerged as new fluorescent probes for biology. When combined with ultrasensitive optical techniques, they allow motions of individual biomolecules to be tracked in live cells with high signal-to-noise and over unprecedented durations. Single QD imaging readily offers a powerful tool to investigate the organization in ...

PURPOSE: To describe ischemic retinopathy in a patient with tetralogy of Fallot. DESIGN: Interventional case report. TESTING: Clinical and imaging evaluation. MAIN OUTCOME MEASURES: Clinical, imaging, and laboratory findings in a patient with tetralogy of Fallot. RESULTS: A 20-year-old female patient with tetralogy of Fallot had progressive visual loss of ...

OBJECTIVES: It is well known that injection of calcitriol (CT) or maxacalcitol (OCT) is very effective in hemodialysis patients with secondary hyperparathyroidism (2HPT). However, it is difficult to use these drugs with peritoneal dialysis (PD) patients with 2HPT because these drugs must be injected two or three times per week. ...

The authors report an unusual case of Parry-Romberg syndrome (PRS). Magnetic resonance imaging and computed tomography scan of the craniofacial region and surgical correction of enophthalmos were performed. Results after the operative intervention included persistent palpebral edema and ecchymosis and transient choroid vasculitis of the right eye, highlighted with echography ...

PURPOSE: To illustrate the utility of wavefront aberrometry in delineating a subtle lenticular abnormality responsible for decreased best-corrected visual acuity in a patient. DESIGN: Case report. METHODS: Clinical data, corneal topography, and wavefront aberrometry with separation of corneal and lenticular components of the higher-order aberrations are analyzed in a patient ...

The choroid plays an important role in supplying nutrients to and removing waste products from the outer region of the retina. Abnormal choroidal blood flow can disrupt normal retinal function and lead to alterations in visual function. Visualization of the choriocapillaris in vivo is a great challenge to understanding its ...

Observations from the Cassini Visual and Infrared Mapping Spectrometer show an anomalously bright spot on Titan located at 80 degrees W and 20 degrees S. This area is bright in reflected light at all observed wavelengths, but is most noticeable at 5 microns. The spot is associated with a surface ...

PURPOSE: To quantify transient serous retinal detachment in classic and occult choroidal neovascularization (CNV) after photodynamic therapy (PDT). DESIGN: Prospective consecutive case series. METHODS: Consecutive patients with classic and occult CNV were examined by optical coherence tomography before PDT and at 2 and 7 days after PDT. RESULTS: In classic ...

We report a case of crush-stenting with Paclitaxel-eluting TAXUS stents in a left anterior descending artery (LAD) bifurcation lesion. In order to obtain precise information on the mid-term neointimal response of the main vessel to this approach, we evaluated the patient angiographically 9 months later, including intravascular visualization with optical ...

Chiral discrimination of malates in aqueous solutions at near-neutral pH is achieved through fluorescence measurement and imaging using the europium-tetracycline complex (EuTc) as a fluorescent probe. The method is based on the significantly different fluorescence properties of the ternary complexes (Eu-Tc-malate) formed between EuTc and the enantiomeric malates. The enantiomeric ...

PURPOSE: Glaucoma is the second cause, after cataract, of world blindness. Approximately half is thought to be primary angle-closure glaucoma (ACG). This review asks whether ACG can be prevented on a population basis. METHODS AND POPULATIONS: Review of published information from the Inuit of Greenland, Canada and Alaska, and descriptions ...

A 65-year-old patient with an idiopathic full-thickness macular hole underwent a vitrectomy, inner limiting membrane (ILM) peel and gas tamponade. The ILM was stained using 0.5% indocyanine green (ICG). Postoperative autofluorescence imaging shows a central focal hyperfluorescence surrounded by a perifoveal hypofluoresecnt area corresponding to the dyed and peeled ILM. ...

BACKGROUND: Quantitative analysis can be used in combination with fluorescence microscopy. Although the human eye is able to obtain good qualitative results, when analyzing the spatial organization of telomeres in interphase nuclei, there is a need for quantitative results based on image analysis. METHODS: We developed a tool for analyzing ...

PURPOSE: To describe optical coherence tomography (OCT) findings in two cases of typical tamoxifen retinopathy. DESIGN: Observational cases report. METHODS: Two patients with tamoxifen retinopathy were imaged with fluorescein angiography and OCT 3. RESULTS: Fluorescein angiography showed foveolar hyperfluorescence. OCT revealed a foveolar cystoid space with focal disruption of the ...

The retinal image of a figure on a slanted picture is narrower than that of a figure on a frontal picture. In this study, the perceived width of various figures (horizontal line segments, ellipses, faces, symbolic faces, and artistic pictures) on a slanted picture plane was measured. The width of ...

Port-wine stain (PWS) birthmarks are congenital, low-flow vascular malformations of the skin. Lasers are the modality of choice for the treatment of PWS birthmarks, and for most patients the pulsed-dye laser in conjunction with epidermal cooling offers the greatest efficacy and safety. Other light devices, including the 532-nm frequency-doubled Nd:YAG ...

Early detection of cancer and its precursors remains the best way to ensure patient survival and quality of life. Our specific aim is to test a multimodality approach to noninvasive diagnostics of oral premalignancy and malignancy. In the hamster cheek pouch model (120 hamsters), in vivo optical coherence tomography (OCT) ...

Primary angle closure glaucoma is a common cause of visual loss worldwide. Gonioscopy and other current methods of assessment of the anatomy of the anterior segment relevant to the diagnosis of this disease, such as ultrasound biomicroscopy, have significant limitations. Anterior segment optical coherence tomography, with its rapid, noncontact, and ...

PURPOSE: The features of late reopening with spontaneous closure in previously repaired macular holes are illustrated with optical coherence tomography (OCT). DESIGN: Observational case reports. METHODS: Case study of two patients with reopened macular holes that developed 7 years after successful macular hole surgery and then spontaneously closed while patients ...

PURPOSE: To evaluate the changes in intraocular pressure (IOP), corneal thickness (CT), and retinal nerve fiber layer thickness (RNFLT) in patients with hypothyroidism before and after treatment. METHODS: A complete ophthalmic examination including visual acuity, IOP, anterior segment, and fundus examination together with CT and RNFLT measurements were performed for ...

PURPOSE: Optical coherence tomography (OCT) can obtain high-resolution, cross-sectional microscopic images of tissue, potentially enabling optical biopsy to substitute for conventional excisional biopsy. We sought to investigate the capability of OCT to image the microstructure of normal and abnormal bronchial tissue. MATERIALS AND METHODS: Equipment: The OCT system was produced ...

AIM: To investigate the potential advantage of an enhanced corneal compensation algorithm (ECC) compared with variable corneal compensation (VCC) in the analysis of scanning laser polarimetric (SLP) images with atypical retardation pattern (ARP). METHODS: SLP-VCC images with ARP (typical scan score (TSS) <80) of one eye of each of 27 ...

Molecularly-specific contrast can greatly enhance the biomedical utility of optical coherence tomography (OCT). We describe a contrast mechanism, magnetomotive OCT (MMOCT), where a modulated magnetic field induces motion of magnetic nanoparticles. The motion of the nanoparticles modifies the amplitude of the OCT interferogram. High specificity is achieved by subtracting the ...

A novel method for the synthesis of highly monodispersed hydrophillic InP-ZnS nanocrystals and their use as luminescence probes for live cell imaging is reported. Hydrophobic InP-ZnS nanocrystals are prepared by a new method that yields high-quality, luminescent core-shell nanocrystals within 6-8 h of total reaction time. Then by carefully manipulating ...

New conductive transparent (CT) probes that can inject currents into nanometer-sized regions and collect light from them have been developed for shear-force atomic force microscopy (SF-AFM) of partially isolative regions. The CT probe consists of a straight elastic silica fiber with one end tapered to a point. The taper is ...

PURPOSE: To determine and compare the shapes of the retinas of emmetropic and myopic eyes. METHODS: Nonrotationally symmetrical ellipsoids were mathematically fitted to the retinal surfaces of 21 emmetropic and 66 myopic eyes (up to -12 D) of participants aged 18 to 36 years (mean, 25.5) using transverse axial and ...

A hard, low-wear probe for contact-mode writing techniques, such as dip-pen nanolithography (DPN), was fabricated using ultrananocrystalline diamond (UNCD). Molding within anisotropically etched and oxidized pyramidal pits in silicon was used to obtain diamond tips with radii down to 30 nm through growth of UNCD films followed by selective etching ...

AIMS: To assess the diagnostic accuracy of optical coherence tomography (OCT), with/without colour fundus photographs, in predicting fundus fluorescein angiography (FFA) findings in patients suspected of having choroidal neovascularisation (CNV). METHODS: A consecutive series of patients suspected of having CNV underwent OCT and stereo FFA images, which were assigned a ...

This study was performed to demonstrate the ability of intravascular optical coherence tomography (OCT) to identify characteristic vascular responses to percutaneous coronary interventions (PCI). OCT provides cross-sectional images of tissue in situ at approximately 10 microm, rendering detailed structural information. Intravascular ultrasound and OCT images were obtained from 10 patients ...

INTRODUCTION: In adults, evaluation of fundus autofluorescence (AF) plays an important role in the differential diagnosis of retinal diseases. The aim of this study was to evaluate the feasibility of recording AF in children and teenagers and to define typical AF findings of various hereditary retinal diseases during childhood. METHODS: ...

A complete methodology has been developed to integrate simultaneous diffuse optical tomography (DOT) and functional magnetic resonance imaging (MRI) measurements. This includes development of an MRI-compatible optical probe and a method for accurate estimation of the positions of the source and detector optodes in the presence of subject-specific geometric deformations ...

AIM: We undertook a national survey to establish the management of dense vitreous haemorrhage without obvious cause. METHODS DESIGN: Cross-sectional anonymous self-reporting survey of ophthalmic practitioners within three target groups: vitreoretinal specialists (VRS), nonvitreoretinal specialists (NVRS), and associate specialists (AS). INTERVENTION: Presentation of the hypothetical scenario of a patient presenting ...

PURPOSE: To report a case in which silicone oil in the eye migrated into the cerebral ventricles and the subarachnoid space. DESIGN: Observational case report. METHODS: A 62-year-old woman presented with proliferative diabetic retinopathy and tractive retinal detachment in her left eye. Par plana vitrectomy and injection of 5,000-centistoke-viscosity silicone ...