Treatment of refractory or relapsed classical Hodgkin lymphoma (HL) remains challenging, but targeted immunotherapy has recently emerged as a potential treatment option for these patients. Although first-generation monoclonal anti-CD30 antibodies proved disappointing, current efforts to modify anti-CD30 antibodies to improve binding of effector cells and enhance activity appears more promising, ...

Benzoylecgonine is a major metabolite of cocaine. We generated hybridoma cells (C1303) producing anti-benzoylecgonine monoclonal antibody (mAb) with a single-chain variable fragment (scFv) and an antigen-binding domain from the C1303 cells. Genes encoding an scFv antibody and constant region (Fc) were amplified from a cDNA library of C1303 cells using ...

The antibody-drug conjugate (ADC) cAC10-vcMMAE consists of the tubulin inhibitor monomethyl auristatin E (MMAE) conjugated to the chimeric anti-CD30 monoclonal antibody cAC10. This ADC potently interferes with the growth of CD30-positive haematological tumours, including Hodgkin lymphoma (HL) and anaplastic large-cell lymphoma. This study found improved antitumour activity in a preclinical ...

Previous studies showed that the HLA class I region is associated with Epstein-Barr virus (EBV)-positive Hodgkin lymphoma (HL) and that HLA-A is the most likely candidate gene in this region. This suggests that antigenic presentation of EBV-derived peptides in the context of HLA-A is involved in the pathogenesis of EBV+ ...

The Human Leucocyte Differentiation Antigens Workshops (HLDA) have since 1984 provided a forum for the characterization and study of leucocyte surface molecules and antibodies against them. HLDA devised the CD nomenclature, which is sanctioned by IUIS. The HLDA Council reviewed and modified the objectives of HLDA in 2004, and changed ...

The 8th International Workshop on Human Leucocyte Differentiation Antigens (chaired by Zola H and managed by Swart B) was run over a 4-year period and culminated in a conference in December 2004. Here we review the achievements of the HLDA Workshops and provide links to information on CD molecules and ...

Monoclonal antibodies submitted to the natural killer (NK) cell section of the Eighth International Workshop on Human Leucocyte Differentiation Antigens (HLDA8) comprised those to known clusters of differentiation (CD), those to well-characterised molecules without a CD nomenclature, and those to unknown molecules. From the HLDA8 workshop, the seven well-characterised molecules ...

Based on the presence of an abundant inflammatory infiltrate, expression of a broad spectrum of cytokines and the professional antigen presenting phenotype of Hodgkin Reed-Sternberg cells it can be anticipated that immunological mechanisms play a major role in the pathogenesis of Hodgkin's lymphoma (HL). Genetic susceptibility to HL probably relates ...

Based on the presence of an abundant inflammatory infiltrate, expression of a broad spectrum of cytokines and the professional antigen presenting phenotype of Hodgkin Reed-Sternberg cells it can be anticipated that immunological mechanisms play a major role in the pathogenesis of Hodgkin’s lymphoma (HL). Genetic susceptibility to HL probably relates ...

The presence and absence of inter-heavy-chain disulphide linkages contribute to the existence of the tetrameric (H(2)L(2)) and half (HL) human IgG molecules, respectively. Reduced effector response in the human IgG4 subclass presents an alternative therapeutic platform in a monoclonal-antibody (mAb) development program. During the initial cell-selection stage, titres of the ...

Many new approaches involving the use of antibody-based agents have produced promising results in experimental Hodgkin's lymphoma (HL) models. Early clinical trials using immunotoxins, radioimmunotherapy (RIT), bispecific molecules and monoclonal antibodies (mAbs), have demonstrated some clinical efficacy in patients with advanced refractory HL. Although it seems unlikely that these approaches ...

This paper reviews the Seventh Human Leucocyte Differentiation Antigen (HLDA7) workshop. Due to the limitations of "blind" antibody screening, which had been evident at the previous meeting in 1996, participants at HLDA7 adopted a more selective approach to the choice of antibodies by identifying new CD specificities. This resulted in ...

Antibodies are poorly transported across cell membranes and biological barriers in vivo. Cationization of antibody molecules by the derivatization of surface carboxyl groups generating primary amino groups could represent a strategy for intracellular antibody delivery. Before cationization of polyclonal colchicine-specific IgG and Fab, using hexamethylenediamine the isoelectric point (pl) of ...

We have established a hybridoma producing monoclonal antibody (MAb) against a linear epitope of glycoprotein (G protein) of the RC-HL strain of rabies virus. This MAb15-13 showed almost the same neutralizing activity to all of five rabies fixed strains, including RC-HL, and reacted to the denatured G protein in western ...

The 5th International Workshop on Human Leukocyte Differentiation Antigens (Boston, USA, 3-7 November 1993) recommended the adoption of 48 new CD clusters and subclusters and the redefinition of 14 previously established clusters. The additions and changes made to the existing CD nomenclature are summarized in a Table.

The screening of a λgt11 library with monoclonal antibodies described here is a relatively uncomplicated procedure, but it requires a little introduction nevertheless. For simplicity, it is assumed that you have in your possession a library that is ready to screen, i.e., either a library bought from a company or ...

This study reports the identification and partial characterization of a novel Mr 105,000 nucleolar antigen (P105) identified by a monoclonal antibody. This monoclonal antibody was obtained when a nucleolar protein extract separated from the immunodominant protein C23 was used as the immunogen. Nucleolar antigen P105 was not detected in normal ...

Oral hairy leukoplakia (HL) is a recently described manifestation of human immunodeficiency virus (HIV) infection in which Epstein-Barr virus (EBV) has been shown to replicate. To seek evidence for a local defect in mucosal immunity, we assessed the presence of epithelial Langerhans cells (LC) in these lesions and in autologous ...

Siblings with IgA nephropathy and diffuse proliferative glomerulonephritis without mesangial IgA deposits (PGN) who had identical HL-A antigens are described. A 21-year-old woman suffered from IgA nephropathy and her 27-year-old sister showed diffuse proliferative glomerulonephritis (PGN) distinct from IgA nephropathy. These siblings had identical HL-A alloantigens which were A2, Aw24; ...

HL-A (A,B,C, and DR) antigen frequency distribution was determined in 29 patients with choriocarcinoma and in their husbands, as well as in a matched control population of 21 healthy couples. All 29 patients had achieved complete sustained remission with chemotherapy. The HL-A antigen frequency distribution was normal in the choriocarcinoma ...

Antiviral activity of interferon secreted by human leukocytes into the culture fluid in the presence of tunicamycin (1-2 microgram/ml) was significantly decreased, by 50-60 percent, in comparison to that produced in the absence of the antibiotic. No loss in antiviral activity occurred when tunicamycin was added to already harvested interferon ...

HL-A antigens were determined in 26 unrelated Japanese patients with hypertrophic cardiomyopathy. Several antigens were more common in patients compared with controls, but statistically significant differences were not evidenced. We also studied two families in which many had a hypertrophic cardiomyopathy. All the affected individuals revealed HL-A-A9 and B7, while ...

82 consecutive patients checked at the out-patient clinic of the Psychiatric Department of the Umeå University were typed for HL-A antigens. The patients belonged to the diagnostic subgroups: bipolar (manic-depressive) psychosis (n = 33), unipolar recurrent depressive psychosis (n = 29) and cycloid psychosis (n = 20) and all had ...

The single radial haemolysis reaction has been shown to be suitable for the quantitative assay of specific antibody to West Nile and Sindbis viruses. Only 5 mul of undiluted serum are needed for the test, which can be performed on crude preparations of antigen and without removal of nonspecific inhibitors. ...

Qualitative and quantitative analysis of the distribution of Hl 1 antigen and R 67 antigen on high density lipoprotein (HDL) particles were conducted on six rabbit sera. The Hl 1 antigen showed non-identity with A-I-containing HDL particles, and antiserum to Hl 1 did not remove A-I-containing HDL particles. The R ...

A modified microassay procedure, using triphenyltetrazolium chloride as a germination indicator, was compared with a macroassay method proposed by the World Health Organization for determining the level of antibodies before and after immunization with Group A meningococcal polysaccharide vaccine. There was excellent agreement between the results obtained by the two ...

Human leukocyte typing sera of known specificities were used to test the leukocyte antigens of vervet monkeys. The results suggest that these leukocytes contained an antigen resembling the HL-A7 antigen of human leukocytes. This is similar to a previous observation with leukocytes from baboons. These findings are consistent with the ...

A patient with psoriasis and anterior uveitis is described. In her family the two entities of iridocyclitis and psoriasis occurred separately in two generations and appeared associated in the third generation. Tissue typing of the family revealed HL-A 27 antigen in two members and HL-A 17 in the other two ...

Antiserum against starfish egg myosin was produced in rabbits. Antibody specificity to myosin was demonstrated by Ouchterlony's immunodiffusion test and by immunoelectrophoresis in the presence of sodium dodecylsulfate (SDS). The latter technique showed that the antibody binds to both heavy and light chains of egg myosin. Furthermore, the antibody reacted ...

HL-A antisera were found to react, in indirect immunofluorescence on human skin, with the cytoplasm of the upper cell layers of the epidermis. It has been established that this staining was not due to an HL-A-antigen-antibody reaction but rather to a contamination of HL-A antisera with non-HL-A epidermal cytoplasmic antibodies. ...

Nineteen vasectomized men were followed for 21 to 42 months after surgery, and their sera were tested for the presence of HL-A lymphocytoxic antibodies. In a previous study, the sera of two of these men had shown a definite increase in serum reactivity 6 to 12 months after surgery. Only ...

The frequency of HL-A phenotypes has been investigated in 64 patients who had recurrent aphthous ulcers and 100 subjects who did not. The HL-A phenotypes were determined by a two-step microcytotoxicity test on peripheral blood lymphocytes, incubating these cells with a range of defined antiserums and complement and subsequently assessing ...

The development of antibodies to Escherichia coli heat-labile enterotoxin (LT) and its relationship to diarrhoea was studied in two Swedish battalions (one in the winter, WB, and one in the summer, SB) of the United Nations Peace-keeping Force in Cyprus. Whereas only 6-15% of the soldiers had detectable levels of ...

Histocompatibility antigen typing was undertaken in 100 Caucasian patients with Graves' disease (autoimmune thyrotoxicosis) and in 100 healthy Caucasian blood donors. Standard lymphocyte microcytotoxicity methods were used to tests for the presence of 22 HL-A antigens in all subjects. There was a significant difference between the groups in respect of ...

HL-A antigens were examined in sixty-two patients, thirty-one boys and thirty-one girls, diagnosed as having JRA. HL-A 27 was the only antigen with a significantly increased frequency. This increase concerned predominantly male patients in whom the disease developed at the beginning of puberty. In this group of sixteen boys, the ...

It is now well established that beta2-microglobulin constitutes one of the two HL-A antigen subunits. In this study support was obtained for the previous notion that the human lymphoma Daudi does not produce beta2-microglobulin (beta2m). Papain-solubilized as well as Nonidet P-40-solubilized Daudi HL-A antigens do not contain any beta2m or ...

To assess the association between open-angle glaucoma and HL-A antigens, the frequencies of 25 HL-A antigens were determined in 49 patients with OAG, and 22 patients with increased intraocular pressure, due to causes other than OAG, using the microcytotoxicity method. The results were compared to 250 individuals from the general ...

Effects of antibiotics and chemotherapeutics on the interaction between the HL-A antigens and the corresponding lymphocytotoxic sera vary from case to case. Streptomycin, oleandomycin, cephaloridine and kanamycin completely inhibit the cytotoxic reaction when added in the first or second stage of the test. Cytembena weakens the reaction while chlortetracyclin exhibits ...

beta2-Microglobulin on the surface of lymphocytes exists in two molecular forms, first as part of the HL-A antigen complex, and second as a free molecule unbound to other membrane macromolecules. Quantitative determinations of the numbers of molecules of beta2-microglobulin per lymphocyte when compared to published values for the numbers of ...

A patient who had received multiple transfusions for the treatment of aplastic anemia was studied. There were antibodies against allogeneic granulocytes and lymphocytes in her serum. Granulocyte antibodies were detected by their effect on phagocytosis-associated hexose monophosphate shunt (HMS) activity, while lymphocyte antibodies were detected by their cytotoxicity. Granulocyte antibodies ...

The effect of antibiotics on lymphocyte HL-A antigens in vitro is of variable character. All antibiotics under examination suppressed the absorption capacity of HL-A antigens after 2 hr of lymphocyte treatment at 37 degrees C. Ceporin and Kanamytrex inhibited even the cytotoxic reactivity of Hl-A antigens after 15-30 min of ...

The relationship between HL-A antigens and rosetting of sheep red blood cells (SRBC) with peripheral human lymphocytes has been investigated by incubating them with HL-A antibodies. Although sensitizing the lymphocytes with HL-A alloantisera had no effect on their ability to form rosettes with SRBC, further sensitization with C6 deficient rabbit ...

In a Caucasian blood donor population largely of Polish and German origin, W25 and W26 were shown to be distinct antigens which comprise the entire specificity of HL--A10. These antigens were in linkage disequilibrium with W18 and W16 respectively. Multiparous women with antibody against HL--A10 (W25 -- W26) had husbands ...

A chromatographical study was made of eight antisera containing either anti-HL-A antibodies, cold lymphocytotoxins, or a mixture of both types of antibodies. The results vary according to the sera obtained. Usually cold lymphocytotoxins are sited in the IgM peak and anti-HL-A antibodies in the IgG peak, but there are some ...

HL-A antigen and gene frequencies of 206 unrelated adults living in the cities of Caracas and Maracaibo have shown that this heterogeneous Venezuelan population sample possess 11 specificities of the first (LA) HL-A series and 15 specificities of the second (Four) series. HL-A2, HL-A9, HL-A5 and W5 showed frequencies higher ...

Twenty-two patients with the Lennox-Gastaut syndrome and their families were examined for HL-A antigens by the microlymphocytotoxicity test. The antigen HL-A7 belonging to the HL-A locus showed a significantly increased frequency (p less than 0.0005) both in parents and in patients. The same antigen showed a significantly altered segregation in ...

Five kinds of fragments of hen egg-white lysozyme (HL) were tested by macrophage migration inhibition (MMI) assay using peritoneal exudate cells (PEC) of guinea pigs immunized with HL in complete Freund's adjuvant. (See article). These four kinds of HL fragments were also shown to be composed of the immunodominant groups ...

Sera, previously found to react specifically with B lymphoid cultured cells, were tested on isolated T and B peripheral blood lymphocytes in a microcytotoxicity assay. Studies were performed on lymphocytes obtained from several large Amish families. The sera used in these studies were cytotoxic to peripheral blood, B lymphocytes, but ...

HL-A 33,000 Dalton fragments were isolated from three papain-solubilized HL-A preparations carrying different HL-A allospecificity by the dissociation induced in the reaction with rabbit antibodies to HL-A 11,000-Dalton fragment (i.e. human beta2-microglobulin). By assaying the binding activity with rabbit antisera against papain-solubilized HL-A molecules and against each of the HL-A ...

The HL-A W27 tissue antigen provides a laboratory tool that has already been of great use in explaining the clinical relationships and overlaps among the true seronegative arthritides. In addition, the W27 antigen is useful to the clinician in differential diagnosis and is becoming more readily available through various laboratories. ...