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Mulquiney P J - - 1999
This is the third of three papers [see also Mulquiney, Bubb and Kuchel (1999) Biochem. J. 342, 565-578; Mulquiney and Kuchel (1999) Biochem. J. 342, 579-594] for which the general goal was to explain the regulation and control of 2,3-bisphosphoglycerate (2,3-BPG) metabolism in human erythrocytes. 2,3-BPG is a major modulator ...
Hanzawa K - - 1999
We examined the relationship in horses between osmotic fragility of erythrocytes (OFE) measured as the red cell haemolysis rates (HLR) in 0.56% NaCl and certain physiological characters. Animals were exercised on a treadmill: warmed up at 30% VO2max for 5 min or at 105% VO2max for 1 min, given an ...
McMahon L P - - 1999
BACKGROUND: To determine the effects of different haemoglobin (Hb) levels on exercise performance and associated electrolyte changes, a prospective, randomized, double-blinded crossover study was completed in 14 haemodialysis patients. METHODS: Performance and changes in arterial [K+] and lactate were compared at rest and during a maximal incremental cycling exercise at ...
Fernandes M A - - 1999
In previous studies performed in this laboratory it was realized that in a broad concentration range (0.5-8 mM) dichromate does not induced red blood cell (RBC) peroxidation. To investigate the reasons behind RBC protection against chromate-induced peroxidation, the effects of 8 mM dichromate on white ghost and RBC peroxidation, RBC ...
Godart H - - 1999
A sudden decrease in external medium osmolality (90 mosmol/kg) causes an immediate swelling of trout erythrocytes, followed by a regulatory volume decrease (RVD) due to activation of both a KCl cotransporter and a taurine transport pathway. Here, we determined how trout red cells respond when they are exposed to a ...
García-Pérez A I - - 1999
The role of molecular crowding and viscosity on the apparent translational diffusion coefficient (ADC) of small metabolites was investigated in different subcellular organelles using the pulse-field gradient spin-echo 1H NMR technique. ADCs of metabolites with increasing radius of gyration (0.7 A < RG < 4.5 A) were measured in the ...
Muñoz A - - 1999
The cardiovascular and metabolic response to two cross-country events (CC*: preliminary level and CC*** advanced level) were analysed in 8 male eventing horses (4 Anglo-Hunter and 4 Anglo-Arabian). This study focused on the establishment of the main metabolic pathways involved in the muscle energy resynthesis during the competitions. Heart rate ...
Molteni S N - - 1999
Hexakis-2-methoxyisobutylisonitrile (SestaMIBI) and 1,2-bis[bis(2-ethoxyethyl)phosphino]ethane (tetrofosmin) are 99mtechnetium compounds widely used in oncologic imaging. We investigated the uptake and release of SestaMIBI and tetrofosmin together with 99mTc-medronate in the MCF7 breast carcinoma cell line. All the tracers showed similar uptake kinetics, with a rapid increase in the first 30 minutes and ...
Khaled S - - 1999
We previously reported a higher blood viscosity at corrected hematocrit (45%) (explained by a higher value of erythrocyte rigidity) in football players with low serum zinc (Zn) and thus presumably Zn deficiency; subjects with low serum zinc had also an impairment in performance. This interventional study was undertaken in order ...
Kobayashi N - - 1998
Podocytes are unique cells that are decisively involved in glomerular filtration. They are equipped with a complex process system consisting of major processes and foot processes whose function is insufficiently understood (Mundel, P., and W. Kriz. 1995. Anat. Embryol. 192:385-397). The major processes of podocytes contain a microtubular cytoskeleton. Taking ...
Bouix D - - 1998
We investigated relationships among body composition, blood rheology, and exercise performance in 14 rugbymen (19-31 yr, weight 65.8-109.2 kg, height 1.7-1.96 m, body mass index 21.7-33.1 kg/m2) who underwent a standardized submaximal exercise session on cycloergometer corresponding to 225 kJ over 30 min. The rheologic response to exercise was measured ...
Brun J F - - 1998
The life-extending effects of regular exercise are related to a decrease in both coronary and peripheral vascular morbidity, associated with some improvements in cardiovascular risk factors. A possible link between the beneficial metabolic and hemodynamic effects of exercise could be blood rheology, which is markedly affected by exercise. We propose ...
Szcześniak L - - 1998
In the present work we investigated the influence of oxidative stress induced by strenuous exercise on the affinity of a specific insulin receptors on human erythrocytes. 13 male members of the national basketball staff performed a maximal treadmill exercise test. In the blood samples collected before and directly after the ...
Mujika I - - 1998
The purpose of this study was to monitor hematological changes during 12 weeks of intense training and 4 weeks of taper in 8 highly trained competitive swimmers, and to assess the relationships between hematological variables and competition performance. Venous blood samples were obtained in the mid-season (wk 10), before taper ...
Vaughn M W - - 1998
Despite its well-documented importance, the mechanism for nitric oxide (NO) transport in vivo is still unclear. In particular, the effect of hemoglobin-NO interaction and the range of NO action have not been characterized in the microcirculation, where blood flow is optimally regulated. Using a mathematical model and experimental data on ...
Ogawa E - - 1998
It was recently coincidentally discovered, using 1H NMR spectroscopy, that the erythrocytes of two species of Australian marsupials, Tammar Wallaby (Macropus eugenii) and Bettong (Bettongia penicillata), contain relatively high concentrations of the essential amino acid lysine (Agar NS, Rae CD, Chapman BE, Kuchel PW. Comp Biochem Physiol 1991;99B:575-97). Hence, in ...
de los Reyes B - - 1998
We studied the effects of L-carnitine treatment in the acyl flux of erythrocyte membranes from uremic patients. We found a significantly lower relative proportion of long-chain acyl-CoA (LCCoA) to free CoA (FCoA) in patients than in control subjects. In addition, patients had reduced activities of both carnitine palmitoyltransferase (CPT) and ...
Sánchez-Galán S - - 1998
In this work we measured both micronuclei number in kidney erythrocytes and fluctuating asymmetry in wild brown trout (Salmo trutta), caught in different fluvial ecosystems of Asturias (northern Spain) characterized by different levels of anthropic influence. Brown trout samples from rivers with high anthropic influence possessed significantly higher averages of ...
Gürcan N - - 1998
The blood stream is affected by viscosity and many other haemorheological factors such as lipid peroxidation in the plasma and red blood cells. The aim of this study was to investigate the changes of haemorheological parameters after submaximal exercise in trained and untrained subjects. The results indicated that heart rate, ...
Spodaryk K - - 1998
The aim of this study was to evaluate the influence of exercise with the intensity progressively increasing from rest until maximal oxygen uptake (VO2max) on 2,3-DPG levels in red blood cells (RBC) in relation to the changes in the acid-base balance and plasma lactate concentration. Six healthy young men (age ...
Duma E - - 1998
Athletes (8 trained medium and long distance runners) have been explored before and after physical exercise under two testing procedures. After the ergometric test significant increases of Ca++ and Mg++ in serum and significant decrease of Mg++ in erythrocytes occurred. A marked increment of the cortisol level and lesser changes ...
De Crée C - - 1997
The present study was designed to assess the effects of acute exercise and short-term intensive training on catechol-O-methyltransferase (COMT) activity. COMT inactivates catecholamines and converts primary catecholestrogens (CE) into their O-methylated form yielding the 2- (2-MeOE) and 4-methoxyestrogens (4-MeOE). Blood samples were obtained from 15 previously untrained eumenorrheic women (mean ...
Speake P F - - 1997
K influx into equine red blood cells (RBCs) was measured using 86Rb as a tracer for K under conditions designed to mimic the changes in respiratory blood parameters that occur in vivo during strenuous exercise. The effects on K influx of physiological changes in pH, cell volume, O2 tension (PO2), ...
Gaffet P - - 1997
The transport of pro-cathepsin D from the trans-Golgi network (TGN) to the endosomal pathway is dependent on binding to the calcium-independent mannose 6-phosphate receptor (ci-M6PR), which is incorporated into TGN-derived clathrin-coated transport vesicles (CCVs). Inhibition of this transport step by wortmannin has led to the proposal that it is dependent ...
Senok A C - - 1997
Knowledge of innate mechanisms of protection against malaria could be used to bolster the existing limited treatments. Oxidant stress may play a role in the protective mechanism and the effect of red blood cell (RBC) age has recently been recognized. This study investigated the role of oxidant stress in the ...
Kijkornphan S - - 1997
Study was conducted to evaluate the effectiveness of Imugard III-RC (4B) to remove leukocyte from packed red cells (PRCs). The leukafiltration set, Imugard III (4B), bedside use for PRCs or whole blood was converted to laboratory use by connecting the distal end of filter tubing to the transfer bag and ...
Heekeren H R - - 1997
Using near-infrared spectroscopy, we investigated the time-course of the concentrations of oxygenated haemoglobin, [oxy-Hb], and deoxygenated haemoglobin [deoxy-Hb], in the occipital cortex of healthy human adults during standard sustained visual stimulation. Within a few seconds after stimulation (by coloured dodecahedron), we observed a decrease in [deoxy-Hb], peaking after 13 s ...
Graff R D - - 1997
The effects of methylmercury (MeHg) on microtubules (MTs) in differentiating neurons derived from retinoic acid-induced embryonal carcinoma (EC) cells in culture were examined by immunofluorescence microscopy. Undifferentiated EC cells contained mostly kinetically labile tyrosinated (TYR) MTs which extended from the centrosome and a small population of stable acetylated (ACT) MTs ...
Yu A W - - 1997
Splanchnic and splenic erythrocyte volumes decrease during postural changes and exercise to help maintain central blood volume and cardiac output. The contribution of this compensatory mechanism to hemodynamic stability during dialysis has not been studied, however. In 8 ESRD patients, age 51.0 +/- 4.5 years old, we measured changes in ...
Waterman-Storer C M - - 1997
Although it is generally believed that microtubules have minus ends bound to the centrosome and free plus ends that exhibit dynamic instability, recent observations show that the minus ends can be free and that modulation of dynamic instability at both ends can result in treadmilling and flux in interphase cells.
Kitagawa Y - - 1997
The present studies were designed to test the hypothesis that mechanical deformation of polymorphonuclear leukocytes (PMN) leads to functional changes that might influence their transit in the pulmonary capillaries. Human leukocytes were passed through 5- or 3-micron-pore polycarbonate filters under controlled conditions. Morphometric analysis showed that the majority of PMN ...
Saghir A N - - 1997
Erythrocyte measures of copper-zinc superoxide dismutase (CuZnSOD) were performed on 11 subjects with a clinical diagnosis of Gilles de la Tourette syndrome (GTS) and 6 healthy controls at specified intervals throughout the day. There were no significant differences between GTS subjects and controls but in both subjects and controls there ...
Gannon B J - - 1997
This study investigated variations in microvascular perfusion of human endometrium across the menstrual cycle, using a laser Doppler technique to assess red blood cell (RBC) flux. Endometrial RBC flux was monitored by laser Doppler fluxmetry via a fibre optic probe inserted transvaginally into the uteri of 19 conscious normal volunteer ...
Khaled S - - 1997
We aimed at investigating relationships between zinc status, blood rheology and blood glucose during exercise. Twenty-one professional football players underwent a triangular maximal exercise test on cycloergometer, with progressively increasing work loads until VO2max. On the whole these subjects had a low serum zinc because nine of them had a ...
Marrades R M - - 1997
The equation proposed by Cotes and coworkers is currently considered as the most acceptable to correct carbon monoxide diffusing capacity (DLCO) for hemoglobin concentration [Hb] by both the American Thoracic Society (ATS) and the European Respiratory Society (ERS) guidelines for standardization of DLCO. In a previous study on 24 anemic ...
McKelvie R S - - 1997
Ion concentrations in whole blood, plasma, and erythrocytes from arterialized venous blood were examined in eight healthy males performing incremental exercise tests to fatigue on an electrically braked cycle ergometer. Exercise was performed during control and low dose (LD) and high dose (HD) of propranolol (beta-blockade). The LD and HD ...
Navas I M - - 1996
The effect of a heterologous series of cationic diamidines has been tested on cell growth and polyamine uptake on the model of insect Trypanosomatid Crithidia fasciculata. The maximum inhibitory effect on both parameters was found for pentamidine and dibromopropamidine, which exhibit a longer distance between amino and imino substituents. A ...
Gabrielson K L - - 1996
Two Hampshire-Duroc cross piglets maintained on 100% total parenteral nutrition (TPN) for 3 weeks developed pancreatic epithelial cell necrosis, diffuse acinar atrophy, and marked interstitial fibrosis. In addition, the piglets had severe villus atrophy in the small intestine as a result of TPN. Atomic absorption spectrophotometric analysis of liver samples ...
Treib J - - 1996
Hydroxyethyl starch (HES) with a high or medium molecular weight (MW) and a high degree of substitution is difficult to degrade and leads to an accumulation of large molecules. These molecules have a negative effect on hemostasiological parameters. In 10 patients with cerebrovascular diseases, a hemodilution therapy was carried out ...
Maw G J - - 1996
Despite evidence of hypervolemia following endurance training, there is little information regarding corresponding extravascular fluid volumes. Quantification of such volumes relies upon radionuclide dilution methods, previously hampered by the loss of plasma albumin. It was our purpose to measure human body-fluid distribution in eight endurance-trained males, using a simultaneous radionuclide ...
Georgopoulos M - - 1996
OBJECTIVE: To determine whether the morphological distinction between 'dysmorphic' and 'eumorphic' erythrocytes in urinary sediment differentiates microscopic haematuria (MH) from reno-parenchymal and post-renal bleeding. MATERIALS AND METHODS: The erythrocyte morphology of 2145 urinary sediments from 1391 patients with MH was evaluated by interference-contrast microscopy and compared with the osmolality, pH ...
Weiss D J - - 1996
OBJECTIVE: To determine whether furosemide treatment altered the blood flow properties and serum and RBC electrolyte concentrations of Thoroughbreds during submaximal treadmill exercise. DESIGN: Thoroughbreds were subjected to submaximal treadmill exercise with and without treatment with furosemide (1 mg/kg of body weight, IV). ANIMALS: 5 healthy Throughbreds that had raced ...
Fontanellas A - - 1996
Recombinant human erythropoietin (r-HuEPO) is being successfully used for the treatment of uremic anemia. Several abnormalities of heme biosynthetic pathway have been described in patients with end-stage renal failure. In this condition, the activity of erythrocyte porphobilinogen deaminase has been found to be slightly increased. If this enzyme were to ...
Piacentini M P - - 1996
The concentration of inositol 1,4,5-trisphosphate (InsP3) in erythrocytes from volunteers has been found to modify following strenuous physical exercise. The basal value was almost regained within some 75 min after the completion of the effort. The concurrent variations of pH and blood lactate have also been evaluated. Our results represent, ...
Brayman A A - - 1996
The tested hypothesis was that ultrasound-induced hemolysis in blood supplemented with a microbubble contrast agent varies with ultrasound intensity and pulse duration. Human erythrocytes in autologous plasma containing 3.6% v:v Albunex microspheres were exposed to 1.07-MHz ultrasound pulses of 5 to 1000 mus at SPTP intensities of 0 to 1100 ...
Hurford W E - - 1996
The spleen of the Weddell seal (Leptonychotes weddelli) may contract and inject red blood cells (RBCs) into the peripheral circulation during diving, but evidence for this hypothesis is indirect. Accordingly, we measured splenic dimensions by ultrasonography, plasma catecholamine concentrations, hemoglobin concentration, and hematocrit in five Weddell seals before and after ...
Corti S - - 1995
To evaluate the intracellular accumulation of norfloxacin in mycobacteria, two methods were used with Mycobacterium smegmatis. A radiometric method (K. V. Cundy, C. E. Fasching, K. E. Willard, and L. R. Peterson, J. Antimicrob. Chemother. 28:491-497, 1991) was used without great modification, but the fluorometric method (P. G. S. Mortimer ...
Vazquez F - - 1995
The acrodermatitis enteropathica (AE) mutation affects intestinal zinc absorption. Our goal was to determine whether the AE mutation affects zinc uptake in human fibroblasts. Zinc uptake was determined during initial rates of uptake (10 min) following incubation in HEPES/saline buffer. Zinc uptake (from 0.25 to 1 microM) into normal fibroblasts ...
Hughes G S GS - - 1995
OBJECTIVE: The objective of this study was to evaluate the exercise capacity of subjects given an autologous transfusion or a polymerized bovine hemoglobin solution to define the pharmacodynamics and pharmacokinetics of a new hemoglobin-based oxygen carrier (HBOC-201). METHODS: Six normal healthy male subjects (ages 25 to 45 years) participated in ...
Pacuszka T - - 1995
In an attempt to define glycolipid functions we have prepared photoactivatable, iodinatable derivative of globoside and used it for photoaffinity labeling of human erythrocyte membranes. Lysogloboside (Gb4Sph) was prepared from globoside through deacylation in methanolic KOH followed by re-N-acetylation of galactosaminyl residue. The NH2 group of sphingosine residue in Gb4 ...
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