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Yubero-Lahoz Samanta S Universitat Pompeu Fabra (CEXS-UPF), Barcelona, Spain; Human Pharmacology and Clinical Neurosciences Research Group, Neurosciences Research Program, IMIM-Hospital del Mar Medical Research Institute, Barcelona, - - 2014
Significant differences have been reported over the years in measuring physiological levels of free circulating serotonin (f5-HT) in platelet-poor plasma (PPP). This work shows that there are crucial pre-analytical factors in sample manipulation that can provoke an artifactual release of 5-HT from platelets, and that, even when the sample is ...
Klasson Stina S Department of Plastic and Reconstructive - - 2014
Abstract A method of breast reconstruction is based on the Deep Inferior Epigastric Perforator (DIEP) technique. Skin and fat are transplanted from the abdomen to the chest; blood vessels are reconnected through microsurgery. Nerves are, however, left unconnected. This study aims to evaluate the blood flow and reinnervation of blood ...
Hajjarian Zeinab Z Wellman Center For Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, - - 2014
Measurement of blood viscoelasticity during clotting provides a direct metric of haemostatic conditions. Therefore, technologies that quantify blood viscoelasticity at the point-of-care are invaluable for diagnosing coagulopathies. We present a new approach, Optical Thromboelastography (OTEG) that measures the viscoelastic properties of coagulating blood by evaluating temporal laser speckle fluctuations, reflected ...
Freckmann Guido - - 2014
Abstract Background: Point-of-care testing (POCT) of blood glucose (BG) is performed by medical personnel in clinical settings as well as by patients themselves for self-monitoring of blood glucose (SMBG) at home. We investigated if a system mainly intended for SMBG by people with diabetes, but also suitable for BG measurements ...
González-Sanchís A A Radiation Oncology Department, ERESA, Hospital General Universitario de Valencia (CHGUV), Avenida Tres Cruces, No. 2, 46014, Valencia, Spain, - - 2014
Radiotherapy (RT) is an essential part of the patient's treatment diagnosed with cancer. Determination of the most common RT secondary effect, the cutaneous toxicity, is usually based on visual rating scales, like Common Terminology Criteria for Adverse Events with an inherent subjectivity. The aim of this work is to perform ...
Elliott Jonathan T JT Department of Medical Biophysics, Western Univeristy, London, Ontario, Canada, N6A 5C1; Imaging Division, Lawson Health Research Institute, London, Ontario, Canada, N6A 4V2. Electronic address: - - 2014
Dynamic contrast-enhanced (DCE) near-infrared (NIR) methods have been proposed for bedside monitoring of cerebral blood flow (CBF). These methods have primarily focused on qualitative approaches since scalp contamination hinders quantification. In this study, we demonstrate that accurate CBF measurements can be obtained by analyzing multi-distance time-resolved DCE data with a ...
Sindhulina C C Bangalore Baptist Hospital, Bangalore, - - 2014
Errors in sample identification contribute to patient mismanagement and erroneous administration of blood and blood products. This report describes the rate of sample identification errors over 2 years in a multispecialty hospital in India and possible measures to decrease these errors. Various measures were taken to reduce the risk of identification ...
Igari Kimihiro K Division of Vascular and Endovascular Surgery, Department of Surgery, Tokyo Medical and Dental University, Tokyo, Japan. Electronic address: - - 2014
To assess the peripheral blood flow of patients with peripheral arterial disease (PAD) by indocyanine green (ICG) arteriography (ICGA) with intraarterial injection of ICG, and evaluate the efficacy of the ICGA tests. This study was undertaken from a retrospective review of patients with PAD underwent ICGA tests during digital subtraction ...
Cinotti E E Department of Dermatology, University Hospital of Saint Etienne, Saint-Etienne, - - 2014
In vivo reflectance confocal microscopy (IVCM) is a new tool for skin microcirculation. However, the measure of quantitative blood cell flow (QBCF) has not been standardized. We studied the inter-investigator and the intra-capillary reproducibility of the manual measure of QBCF on IVCM videos and investigated if a software program might ...
Angarita-Jaimes N N School of Mechanical Engineering, University of Leeds, Leeds, UK. Electronic address: - - 2014
Cerebral autoregulation (CA) mechanisms maintain blood flow approximately stable despite changes in arterial blood pressure. Mathematical models that characterise this system have been used extensively in the quantitative assessment of function/impairment of CA. Using spontaneous fluctuations in arterial blood pressure (ABP) as input and cerebral blood flow velocity (CBFV) as ...
Masunaga Shinichiro S Graduate School of Health Sciences, Kumamoto University, Kuhonji 4-24-1, Kumamoto 862-0796, - - 2014
Non-invasive quantitative measurements are useful for clinical study as these are simple and pain-free procedures. A new non-invasive semi-automatic quantitative measurement method, the improved brain uptake ratio (IBUR) method using (99m)Tc-ECD SPECT, has recently been reported. If an automatic ROI setting algorithm could be developed to determine the input function ...
Sattarivand Mike M Department of Medical Biophysics, University of Toronto, Odette Cancer Centre at Sunnybrook Health Sciences Centre, Toronto, Ontario, - - 2014
The purpose of this study was to assess the accuracy and practicality of well counter- and thyroid probe-based methods, commonly available in nuclear medicine facilities, for measuring the concentration of (18)F-FDG in blood samples. The degree to which the accuracy of such methods influences quantitative analysis of dynamic PET scans ...
Yoshino K K Department of Chemistry, Faculty of Science, Shinshu University, Asahi, Matsumoto, Nagano 390-8621, Japan. Electronic address: - - 2014
First spontaneous, noninvasive determination method of (10)B-BPA, (10)B-BPA-fructose complex, and total (10)B in blood is described. (10)B-NMR measurement with 100,000 FT accumulation enables us to obtain the result within 100min/sample. The detection limits for the simultaneous analysis were 3ppm, 3ppm and 6ppm for (10)B-BPA, (10)B-BPA-fructose complex and total (10)B respectively ...
Domori Asuka A Laboratory of Small Animal Internal Medicine, Joint Faculty of Veterinary Medicine, Kagoshima University, Korimoto, Kagoshima, - - 2014
Portable blood glucose meters (PBGMs) are useful for serial measurements of blood glucose and creation of blood glucose curves in veterinary practice. However, it is necessary to validate PBGMs designed for people for veterinary use. Our objective was to evaluate the accuracy of 2 PBGMs designed for people for use ...
Tiftik Tülay T Ankara Physical Medicine and Rehabilitation Training and Research Hospital, Ankara, - - 2014
To evaluate blood flow of hand arteries (using Doppler ultrasonography) and sympathetic skin response (SSR) in patients with hemiparesis. Fifty-six stroke patients (30 M, 26 F) with unilateral hemiparesis (age 53.5 ± 10.8 years, mean disease duration 12.0 ± 19.1 months) were included. The patients' arm and hand motor functions were assessed according to ...
Ives Stephen J SJ University of - - 2014
Ultrasound Doppler and near infrared spectroscopy (NIRS) are routinely used for non-invasive monitoring of peripheral hemodynamics in both clinical and experimental settings. However, the comparative ability of these methodologies to detect changes in microvascular and whole-limb hemodynamics during pharmacologic manipulation of vascular smooth muscle receptors located at varied locations within ...
Zuzarte-Luis Vanessa - - 2014
The first phase of malaria infection occurs in the liver and is clinically silent. Inside hepatocytes each Plasmodium sporozoite replicate into thousands of erythrocyte-infectious merozoites that when released into the blood stream result in clinical symptoms of the disease. The time between sporozoite inoculation and the appearance of parasites in ...
Mahe Guillaume G Gonda Vascular Center, Mayo Clinic, Rochester, Minn; L'Université Nantes Angers Le Mans University, Institut National de la Santé et de la Recherche Médicale 1083/CNRS 6214, Faculty of Medicine, Angers, France. Electronic address: - - 2014
This study was conducted to determine the sensitivity and specificity of laser Doppler flowmetry (LDF) measurements for digital obstructive arterial disease (DOAD) using angiography as the reference standard and to compare the accuracy of different classical tests used to assess DOAD. Diagnosis of vascular abnormalities at the digital level is ...
Tajima Yosuke Y Biophysics Program, Molecular Imaging Center, National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, - - 2014
Laser-Doppler flowmetry has been widely used to trace hemodynamic changes in experimental stroke research. The purpose of the present study was to evaluate the day-to-day test-retest reproducibility of measuring cerebral blood flow by LDF in awake mice. The flux indicating cerebral blood flow (CBF), red blood cell (RBC) velocity, and ...
Fieramonti Luca L Politecnico di Milano, Dipartimento di Fisica, piazza Leonardo da Vinci 32, Milano, - - 2013
Microscopy techniques can readily visualize the finest details of embryo vasculature, but still lack to provide a complete three-dimensional representation of blood flow parameters. We present an in-vivo 3D imaging technique, able to reconstruct the blood cell velocity vector over a large volume of zebrafish embryos. This low cost and ...
Petersen Nils H - - 2013
There is a growing interest in measuring cerebral autoregulation in patients with acute brain injury. Non-invasive finger photo-plethysmography (Finapres) is the method of choice to relate arterial blood pressure to changes in cerebral blood flow. Among acutely ill patients, however, peripheral vasoconstriction often limits the use of Finapres requiring direct ...
Rud B - - 2013
We evaluated arm and leg work rate and metabolism during double pole ergometer skiing. Thermodilution arm and leg blood flow was determined together with the arterial to venous difference for oxygen, while the work rate was assessed in eight male recreational skiers [24 (SD 7) years]. When work rate increased ...
Storm A C - - 2013
Salivary flow and the net jugular flux of metabolites were studied during resting and rumination in three lactating dairy cows (BW 548 ± 17.2 kg, days in milk 113 ± 4 d). The method was based on the concentration difference between arterial and jugular blood, and jugular blood flow measured ...
Zhou Xi - - 2013
A consensus has formed that patients with invasive moles should continue with one to three cycles of chemotherapy after human chorionic gonadotropin (hCG) levels have decreased to a normal level. However, the management plan has not been agreed for cases where Doppler ultrasonography (DU) indicates blood-flow signals within the tumor ...
Lamberts Robert P - - 2013
Iliac blood flow restrictions causing painful and "powerless" legs are often attributed to overtraining and may develop for some time before being correctly diagnosed. In the current study, differences between actual performance parameters and performance parameters predicted from the Lamberts and Lamberts Submaximal Cycle test (LSCT-predicted) were studied in a ...
Bi Renzhe - - 2013
Recently, diffuse speckle contrast analysis (DSCA) was introduced as a competent modality for deep tissue blood flow measurement, where the speckle contrast is calculated over spatial domain on the CCD image of diffuse reflectance. In this paper, we introduce time-domain DSCA where temporal statistics are used for speckle contrast calculation ...
Smits Simone M - - 2013
Differential counting and morphological analysis of peripheral blood smears is of great diagnostic importance to the clinician. For economic and time-saving reasons, automated imaging processes have been successfully introduced over the years. The aim of this study was to investigate whether a new morphology system, the CellaVision Image Capture System ...
Tagaya M - - 2013
Cardioplegic solutions often cause high blood concentrations of potassium. The conventional hemoconcentration circuit was improved to correct electrolyte imbalances through a method involving dilutional ultrafiltration (DUF) and an alternative hemodialysis (ALTHD) method. This study aimed to determine the effectiveness of this ALTHD method. Bovine blood was used, in conjunction with ...
Pottgiesser Torben - - 2013
During the last 30 years, the artificial increase of red blood cell volume ("blood doping") has changed the level of performance in all endurance sports. Many doping scandals have shown the extent of the problem. The detection of blood doping relies on two different approaches: the direct detection of exogenous manipulating ...
Taghiloo Maryam - - 2013
We consider a low-Reynolds-number swimmer made from three spheres linked by two slender arms, and explore its motion in a shear flow. This rodlike three-sphere swimmer finally follows the direction dictated by the shear flow. To overcome this shortcoming, we propose a model in which the two outer spheres have ...
Fujino Yasuhito - - 2013
A novel hematology analyzer for small animal medicine, ProCyte Dx, was developed from combination of the fluorescence laser flow cytometry and laminar flow impedance technologies, and its accuracy was evaluated by comparing with the conventional impedance-based hematology analyzer, pocH-100iV Diff, or microscopic manual cell counting methods with staining blood smears ...
Yang Xiaoxi X Department of Biomedical Engineering, Tulane University, New Orleans, - - 2013
The measurement of hemoglobin concentration ([Hb]) is performed routinely as a part of a complete blood cell count to evaluate the oxygen-carrying capacity of blood. Devices currently available to physicians and clinical laboratories for measuring [Hb] are accurate, operate on small samples, and provide results rapidly, but may be prohibitively ...
Cora Michelle C - - 2013
Objective-To determine the frequency of clinically relevant abnormalities missed by failure to perform a blood smear evaluation in a specific subset of dogs receiving chemotherapy and to compare automated and manual neutrophil counts in the same population Design-Retrospective case series Animals-50 dogs receiving chemotherapy with a total nucleated cell count ...
Ma Yan - - 2013
Objective: A simple method of using fingerstick blood glucose (FSBG) monitors to estimate blood ascorbate values after high-dose intravenous (IV) ascorbate infusion is evaluated as a substitution for high-performance liquid chromatography (HPLC) measurement. Methods: In 33 participants, readings from FSBG monitors were taken before and after IV ascorbate infusions at ...
Frankel Timothy L - - 2013
BACKGROUND: Acute normovolemic hemodilution (ANH) decreases transfusion rates but adds to the complexity of anesthetic management during hepatectomy. A randomized controlled trial was conducted to determine if selecting patients for ANH using a transfusion nomogram improves management and resource use compared with selection using extent of resection. STUDY DESIGN: One ...
Doré V - - 2013
The objective of this cross-sectional study was to validate the accuracy of a hand-held electronic on-farm test (Precision Xtra) for quantifying the blood β-hydroxybutyrate (BHBA) concentration in dairy goats. A total of 114 dairy goats from 3 commercial herds were sampled once for blood in the jugular vein between 1 ...
Vent-Schmidt Jens - - 2013
The aim of this study was to test the accuracy of viscosimetric method to estimate the red blood cell (RBC) deformability properties. Thirty-three subjects were enrolled in this study: 6 healthy subjects (AA), 11 patients with sickle cell-hemoglobin C disease (SC) and 16 patients with sickle cell anemia (SS). Two ...
Zeigler Brandon M - - 2013
Analysis of peripheral blood leukocyte populations by flow cytometry in adult beagles is a critical component of immunotoxicity assessment in regulated pre-clinical toxicology studies. In this study, data is presented utilizing a single panel, six-color method to simultaneously enumerate absolute cell counts and determine the relative percentage of leukocytes. A ...
Tendl Kristina A - - 2013
Abstract Background: Monitoring of blood glucose in neonatal intensive care unit (NICU) patients is important in maintaining normoglycaemia and reducing the risk of hypoglycaemia. Point-of-care testing (POCT) glucose meters provide short turnaround times but some have been reported to be affected by haematocrit interference and other biochemical or biological substances ...
Amin Emily N - - 2013
BACKGROUND: Endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) is being increasingly used in the sampling of pulmonary masses and mediastinal lymphadenopathy. The blood clot core (BCC) often obtained during EBUS-TBNA may not be a true core and therefore may not be submitted for histological analysis. The frequency in which the blood ...
Fujihara Junko - - 2013
The measurement of blood carboxyhemoglobin (CO-Hb) is important to determine the cause of death. The AVOXimeter 4000 (AVOX), a portable CO-oximeter, has the advantages of a low purchase price and operating cost, ease of operation, and rapid results. Little information is available on the usefulness of AVOX in the forensic ...
Campos Derbis - - 2013
OBJECTIVES: The aim of this study was to validate an ultramicroassay with a reduced interference of hemoglobin for the enzymatic diagnosis of mucopolysaccharidosis I in dried blood spots on filter paper. DESIGN AND METHODS: A matrix of dried blood was incorporated within the calibration system. In addition, trichloroacetic acid was ...
Inoue Shigeaki - - 2013
INTRODUCTION: Glucose control to prevent both hyperglycemia and hypoglycemia is important in an intensive care unit. Arterial blood gas analyzers and glucose meters are commonly used to measure blood glucose concentration in an intensive care unit; however, their accuracies are still unclear. METHODS: We performed a systematic literature search (January ...
Liu Xuan - - 2013
We propose an inter-Ascan speckle decorrelation based method that can quantitatively assess blood flow normal to the direction of the optical coherence tomography imaging beam. To validate this method, we performed a systematic study using both phantom and in vivo animal models. Results show that our speckle analysis method can ...
Xu Yanping - - 2013
We have developed a Quantitative Light Absorption Analysis (QLAA) method to rapidly estimate human lymphocyte concentrations isolated from small volumes of whole blood. Measurements of the light absorption analysis were calibrated for lymphocyte concentration levels using a hemocytometer. To validate the QLAA system, blood samples were collected from 17 healthy ...
Grecu Daniela Stefania - - 2013
OBJECTIVES: Estimation of the reference intervals for erythrocyte parameters of neonates in the venous blood, in their first day of life, aiming to achieve quality assurance in post-analytical phase according to the requirement of the ISO standard 15189:2007: Medical Laboratories - Particular Requirements for Quality and Competence. DESIGN AND METHODS: ...
Demaret Julie - - 2013
BACKGROUND: By measuring multiple parameters on a single-cell basis, flow cytometry is a potent tool to dissect the phenotypes and functions of cell subsets. However, because this technique may be time-consuming, particularly for intracellular staining, it could be problematic for its use in daily routine or in large cohorts. Recently, ...
Jenkins Nathan T NT Department of Biomedical Sciences, University of Missouri, Columbia, MO, USA. - - 2013
There is strong and consistent evidence from in vitro studies that disturbed blood flow produces a proatherogenic vascular endothelial phenotype. However, data from human studies are lacking. To address this, a 220 mm Hg occlusion cuff was placed on the distal forearm of 10 young, healthy men to induce a ...
Makovický Peter - - 2013
Bloodstain pattern analysis (BPA) is a valid forensic method which belongs to the category of biological methods using trigonomic models. Despite its development through the years, the method has been re-formulated a standard one and globally used, recognized in standard sheets. This method permits exact analysis of the dynamic and ...
Kamshilin Alexei A - - 2013
The non-invasive assessment of blood flow is invaluable for the diagnostic and monitoring treatment of numerous vascular and neurological diseases. We developed a non-invasive and non-contact method of blood pulsation imaging capable of visualizing and monitoring of the two-dimensional distribution of two key parameters of peripheral blood flow: the blood ...
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