The protamination of spermatozoa recovered by Percoll gradient and swim-up was investigated by means of chromomycin A3 (CMA3) staining. A total of 34 semen samples from patients undergoing IVF treatment were divided in two groups: normal (A) and oligoasthenozoospermic (B). Samples were divided in fractions, subjected to both techniques of ...

OBJECTIVE: To assess the effects of graded intramedullary reaming and nailing on the healing pattern of segmental diaphyseal fractures using male Wistar rats. STUDY DESIGN: In male Wistar rats we produced two standardized, partial osteotomies with an eight-millimeter intermediary fragment in the femoral diaphysis. The osteotomies were subsequently manually broken. ...

The regional blood flow of 30 distraction segments in 27 patients was measured during distraction osteogenesis. There were three groups of patients. Group A consisted of seven patients with high grade malignant tumors who received chemotherapy preoperatively and postoperatively. Group B consisted of eight patients with low grade malignant and ...

Microcirculatory vasomotor responses to an alpha-adrenergic agonist and an antagonist were assessed in 11 familial dysautonomia and nine control subjects by laser Doppler flowmetry. Using two iontophoresis machines, blood flow in the midclavicular areas was continuously monitored by two channel laser Doppler flowmeter. Simultaneously, the alpha-antagonist (0.5 mM phentolamine hydrochloride) ...

We studied the effects of thrombin generation due to surgical stress on prostanoid formation and peripheral circulation in anesthetized dogs. Three experimental groups were used, consisting of a control group (group 1), a thoracotomized group (group 2), and a thoracotomized group treated with thrombin inhibitor (MD805: a synthetic arginine derivative) ...

The effect on the jejunal hemodynamics of restoration of blood flow of the jejunal artery or vein in the free jejunal flap has not yet been elucidated. In the present study, a model of ischemia and reperfusion in the jejunal flap of rabbits, and that of venous congestion and reperfusion, ...

PURPOSE: Keratoconus is characterized by central thinning and an increased curvature of cornea. Scarring of cornea accompanies the disease as it proceeds, and the ultimate treatment is keratoplasty. The pathogenesis of keratoconus is still unknown, and therefore we studied normal, keratoconus, and scarred corneas by a panel of lectin conjugates. ...

Neutral oligosaccharides in human milk samples from approximately 50 women were analysed applying a recently developed high-pH anion-exchange chromatographic method. Three different oligosaccharide patterns could be detected in accordance with milk groups that had been already described. These oligosaccharide groups correspond to the Lewis blood types Le(a-b+), Le(a+b-) and Le(a-b-). ...

Two Baird's tapir (Tapirus bairdii) calves born at the Columbus Zoo from the same sire and dam developed hemolytic anemia that was consistent in history and clinical signs with neonatal isoerythrolysis (NI). One calf developed severe, fatal hemolytic anemia after being fed maternal colostrum, and the other developed moderate hemolytic ...

The lipopolysaccharide of Helicobacter mustelae type strain ATCC 43772 was obtained by phenol-water extraction of bacterial cells. Structural investigations were made on the lipid A free saccharide moiety released from the lipopolysaccharide by mild acetic acid hydrolysis. Nuclear magnetic resonance, gas liquid chromatography-mass spectrometry and fast atom bombardment-mass spectrometry were ...

PURPOSE: We determine the adverse effects of 2 different techniques of vasography in an animal model. MATERIALS AND METHODS: Unilateral vasography was performed by a direct puncture technique with a lymphangiogram needle or through a partial thickness vasostomy technique in 2 groups of 10 adults Lewis rats using nonionic contrast ...

We studied the effect of dopamine on hepatic blood flow during epidural anesthesia with the infusion of hydroxyethyl starch (HES). Hepatic blood flow was measured noninvasively via indocyanine green (ICG) clearance (indices: K [ICG disappearance rate] and R15 [15-min ICG retention rate]). Group C (n = 7) received no epidural ...

Until the '80s blood group reagents were composed of human or animal polyclonal antibodies; nowadays they are mainly produced from monoclonal antibodies. In this paper two aspects will be considered; firstly the evolution in the use of monoclonal reagents in France and secondly the quality of these new reagents in ...

OBJECTIVE: Our purpose was to determine the efficacy of the heteroduplex generator in the prenatal analysis of rhesus CcDEe blood groups. STUDY DESIGN: A cross-sectional study was performed evaluating fetal samples from 85 women undergoing prenatal diagnosis and comparing the results with standard immunologic serotyping on cord blood delivery. RESULTS: ...

BACKGROUND: Traumatic brain injury (TBI) induces an acute inflammatory response characterized by early recruitment of inflammatory cells (white blood cells). Rapid resuscitation of TBI with hypertonic saline/dextran (HS/DEX) yields promising results in clinical and experimental studies. The purpose of this paper was to test the hypothesis that HS/DEX exerts its ...

12 blood-phobic subjects, selected according to the Fear Survey Schedule and the Mutilation Questionnaire, and 50 control subjects performed a paced respiration task during which heart rate and respiration were recorded. Respiratory Sinus Arrhythmia (RSA) was analyzed as an autonomic index of vagal influence on the heart. Analysis showed a ...

Defect in degradation of blood group B-immunoactive glycosphingolipids in Fabry disease (deficiency of lysosomal alpha-galactosidase EC 3.2.1.22) has been studied using highly sensitive and specific TLC-immunostaining analysis of urinary sediments and tonsillar tissues of blood group B patients and healthy controls, secretors and nonsecretors. The B glycolipid antigens with hexasaccharide ...

Blood samples from 15,426 blood donors from 17 out of Norway's 19 counties were tested for the presence of Kell (k) antigen. The K+ frequency in the total series was 8.28%, ranging from 4.61% in East-Agder county to 10.36% in Sogn and Fjordane. A1A2BO grouping of the donors showed that ...

The purpose of this study was to evaluate the effect of low-level laser therapy (LLLT) on sutured wounds of the teat in dairy cattle. By using the Latin square design, the effect of LLLT was evaluated by radiography, measurement of microcirculation flow, histopathology, tensiometry, and hydroxyproline analysis. Sixteen teats of ...

Duffy blood groups were serologically investigated in 434 individuals from Black Lahu (N = 54), Shan (N = 62), Lisu (N = 74), Red Karen (N = 112), White Karen (N = 102) and Manni (N = 30) in Thailand. High frequency of Fya (0.917-1.0) which is comparable with other ...

The introduction of molecular biology techniques to the field of transfusion medicine has allowed more detailed analysis of the basis for the expression of several blood-group antigens. The application of the polymerase chain reaction for the determination of red-cell blood-group genotype is a highly sensitive technique that can be used ...

The purpose of this study was: 1) to investigate the responses of heart rate (HR), blood lactate and ratings of perceived exertion (RPE) to 1,000 punches and 1,000 kicks in collegiate highly skilled (BB Group) and novice (WB Group) karate practitioners; and, 2) to compare RPE obtained from the subjects ...

Fc gamma receptors (Fc gamma R) on white blood cells and the Duffy blood group antigens on red blood cells are coded for on the long arm of chromosome 1. They appear in different allotypic forms: the high responder/low responder (HR/LR) forms of Fc gamma RIIa, the alloantigens NA1 and ...

Maldistribution of multidose blood cardioplegia (BCP) continues to be a problem causing perioperative subendocardial damage. However, it is not known whether alpha-adrenergic stimulation contributes to the maldistribution of multidose BCP. We have evaluated the effects of phenoxybenzamine (POB) on multidose BCP distribution, with a right heart bypass model in 14 ...

In the blood collection practice there is a growing number of different closed systems. They often use plastic pipettes for testing the erythrocyte sedimentation rate (ESR) according to Westergren. Our previous experience with plastic pipettes as a part of the system Venoject II suggested that slightly higher values were obtained ...

The distribution of AB0 blood and Lewis blood group phenotypes in 2988 Greek individuals was determined with monoclonal reagents and the results analysed according to their birthplace or that of their parents when they were born in the same region. Overall, the AB0 blood groups distribution was similar to that ...

The role of ABO blood groups in the carriage rate of dermatophytosis was studied. Blood grouping was done for 108 culture-proven dermatophytosis patients. Forty-nine patients belonged to blood group A, 54 to blood group O, three to blood group B and two to blood group AB. The incidence of dermatophytosis ...

A study was conducted in patients undergoing surgery for idiopathic scoliosis, to determine whether combining blood-saving methods would decrease the need for homologous blood. Five groups were compared in a prospective, randomized fashion. In control patients (n = 13), blood loss was replaced by colloids. Preoperative haemodilution (PHD group) was ...

OBJECTIVES: Blood group antigens traditionally have been associated with a risk of developing peptic ulcer and gastric cancer. Helicobacter pylori is a bacterium associated with chronic active gastritis and ulcer disease, and its attachment to gastric mucosa was recently shown in vitro to be mediated by blood group Lewisb and ...

Little is known about the cerebral electrical response to short periods of hypoxemia, hypotension and their combination. These conditions occur frequently in critically ill newborn infants; their cerebral electrical activity can be registrated easily with the Cerebral Function Monitor (CFM). Therefore we recorded on-line cortical electrical activity during hypoxemia and ...

A new generation of perfluorocarbon emulsions is being clinically evaluated as erythrocyte substitutes. However, the effect of perfluorocarbon emulsions on optical densitometric measurements of percent oxyhemoglobin (%O2Hb) has not been fully characterized. The purpose of this study is to quantify the effect of blood perfluorochemical concentration and hematocrit (Hct) on ...

In this study, the effect of free oxygen radicals on lipid peroxidation and the antioxidant role of alpha-tocopherol (vitamin E) in these reactions were investigated in haematoma fluid and venous blood samples in rabbits with femoral fracture. There were 21 male rabbits, divided into 3 groups. Conjugated dienes values (as ...

BACKGROUND: Hypothermia occurs frequently in clinical anesthesia. Many apparatus were introduced to keep intravenous fluid warm. But at low to medium flow rates little effects have been achieved because of heat lost along the tube. Superlon, an industrial heat-preserving material widely used in air conditioning to prevent temperature change, has ...

The dynamic response of cerebral autoregulation to spontaneous changes in arterial blood pressure (ABP) is described by the relationship between cerebral blood flow velocity (CBFV) and resistance-area product (RAP). CBFV was measured with Doppler ultrasound in the middle cerebral artery and ABP with an intra-arterial catheter in 66 neonates. Spontaneous ...

OBJECTIVE: This study was conducted to investigate biologic responses of the pulp to single-tooth dento-osseous osteotomy and to explore an appropriate level of transverse bone resection for maintaining biologic activity of the pulp in monkeys. STUDY DESIGN: The levels of transverse bone resection were designed at approximately 10 mm (10 ...

Earlier studies have demonstrated a correlation between tortuosity and atherosclerosis in the femoral artery. One conceivable explanation is that atherosclerosis causes an elongation of the artery, resulting in vessel tortuosity, another is that blood flow phenomena (such as flow separation) due to the vessel geometry may affect the progression of ...

PURPOSE: In earlier studies, it has been documented that maxillary gingival blood flow (GBF) decreased significantly during the intraoperative course of a Le Fort I osteotomy. It was not clear, however, whether the observed decrease in GBF was caused by the osteotomy or by the use of local anesthetic with ...

The Kell blood group system is important in transfusion medicine, and the Kell antigen (K1) is probably second in importance to Rh D as an immunogen in alloimmunized pregnancies which cause haemolytic disease of the newborn. The K/k (K1/K2) blood group polymorphism has been recently defined. A point mutation changes ...

The objective of this study was to determine whether hemodilution could normalize the mean transit times of red blood cells (Tr) and plasma (Tp) through cerebral microvessels in a partially ischemic brain. Wistar-Kyoto (WKY) rats, aged 30-40 weeks, were divided randomly into three groups. The first group was the nonocclusion, ...

Serial blood phosphine (PH3) levels were done in patients with severe (Group I, n = 30), mild (Group 2, n = 10) and minimal or nil toxicity due to aluminium phosphide compound. Blood phosphine levels were significantly higher (p < 0.001) in patients of Group I than other two groups. ...

Twin blood group chimerism seems to be very rare in humans. The 30-40 previously reported cases usually were found by mere coincidence during routine blood grouping in hospitals or blood banks. Usually in these cases frank blood group mixtures of, for example, 50/50%, 25/75%, or 5/95% at most were seen. ...

Pronase is a useful and relatively nonspecific protease that cleaves many red blood cell (RBC) membrane proteins that carry blood group antigens. Unexpected findings in tests using pronase-treated RBCs during the investigation of a patient's blood sample led us to test which high-incidence blood group antigens were sensitive and which ...

OBJECTIVES: Bacterial attachment is a prerequisite for colonization of the gastric epithelial surface. Recently, it was demonstrated that the receptor for Helicobacter pylori is the blood group antigen Lewis b, which is exposed only in blood group O. We prospectively examined the prevalence of blood groups in H. pylori-positive and ...

During the 1920s and 1930s the resting of blood groups for large numbers of people became a very common practice. Although much of this was to ensure compatibility for blood transfusion, over 1,000 articles were published with results of tests on over 1.3 million people to answer more theoretical, scientific ...

Anesthetized mongrel (weight range: 16-27 Kg) dogs were prepared for monitoring hemodynamics, blood flow distribution, plasma colloid osmotic pressure and renal functional parameters at various intervals. Removal of 35 ml/Kg blood resulted in marked drop and only partial spontaneous recovery in systemic and pulmonary arterial pressures, cardiac output and organ ...

The role of oxygen free radicals (OFR) in the pathogenesis of many diseases is known. This experimental study was planned to investigate the role of OFR in acute abdominal inflammation. In this study, 20 adult rabbits were used. They were divided into two groups with 10 rabbits in each. In ...

Determination of the carboxyhemoglobin (COHb), methemoglobin (MetHb), and blood lactate concentrations is known as important contributive in evaluation of oxygen deficiency in people from urban populations. The methemoglobinemia is usually caused by unfavourable impact of NOx. A quantification of COHb allows, to some extend, to evaluate the threat of population ...

MATERIALS AND METHODS: In a prospective randomized study we investigated the effect of hemodilution on cefuroxime levels and bacterial contamination of processed shed blood during hip arthroplasty. 10 patients received cefuroxime 1,5 g as single shot prophylaxis before (group A), 10 after hemodilution (15 ml/KG) (group B). Cefuroxime levels in ...

Although there are probably over a thousand publications on the associations of blood groups and disease, many are based totally on statistical analyses. Most of the earlier studies have been controversial, because they were small studies and/or had inadequate controls and/or had been analyzed incorrectly. Nevertheless, it is difficult to ...

Delay in the diagnosis of spermatic cord torsion (SCT) is still a significant cause of testicular loss in children. The aim of this experimental study was to assess the diagnostic value of serum creatine kinase (CK) in the early period following SCT. Forty male rats were assigned randomly into five ...