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Bergonzi Karla M KM Department of Biomedical Engineering, Washington University in St Louis, St Louis, Missouri, - - 2014
Brain function can be assessed from resting-state functional connectivity (rs-fc) maps, most commonly created by analyzing the dynamics of cerebral hemoglobin concentration. Here, we develop the use of Laser Speckle Contrast Imaging (LSCI) for mapping rs-fc using cerebral blood flow (CBF) dynamics. Because LSCI is intrinsically noisy, we used spatial ...
Chapman Michael R MR School of Chemistry, University of Leeds, Woodhouse Lane, Leeds, LS2 9JT, UK. - - 2014
An electrochemical flow-cell for highly efficient and selective generation of Cu(I)-N-heterocyclic carbene complexes under neutral and ambient conditions is reported. The feasibility of the flow-cell is demonstrated through the electrochemical synthesis of [Cu(IMes)Cl] and subsequent in situ flow directly into hydrosilylation reactions, with equal efficiency to the purified catalyst.
Henkelman S S Department of Biomedical Engineering, University Medical Center Groningen, University of Groningen, Groningen, the - - 2014
Cryopreserved (frozen) red blood cells have been used in transfusion medicine since the Vietnam war. The main method to freeze the red blood cells is by usage of glycerol. Although the usage of cryopreserved red blood cells was promising due to the prolonged storage time and the limited cellular deterioration ...
Henshall Tanya L TL From the Department of Cell and Molecular Biology (T.H., S.J., U.L.) and Department of Medical Biochemistry and Biophysics, Division of Vascular Biology (C.B., E.R.), Karolinska Institute, Stockholm, Sweden; Department of Immunology, Genetics, and Pathology, Rudbeck Laboratory, Uppsala University, Uppsala, Sweden (A.K., L.H., E.R., M.A.M., C.B.); EM Unit, Institute of Biomedicine, University of Gothenburg, Gothenburg, Sweden (B.R.J.); and Octapharma AB, Stockholm, Sweden - - 2014
Vascular smooth muscle cells (VSMC) are important for contraction, blood flow distribution, and regulation of blood vessel diameter, but to what extent they contribute to the integrity of blood vessels and blood-brain barrier function is less well understood. In this report, we explored the impact of the loss of VSMC ...
Hou Jianwen J State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China. shiqiang@ciac.ac.cn - - 2014
A novel hydrophilic PAMPS-PAAm brush pattern is fabricated to selectively capture blood cells from whole blood. PAMPS brushes provide antifouling surfaces to resist protein and cell adhesion while PAAm brushes effectively entrap targeted proteins for site-specific and cell-type dependent capture of blood cells.
Akeju Oluwaseun O Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, United - - 2014
Understanding the neural basis of consciousness is fundamental to neuroscience research. Disruptions in cortico-cortical connectivity have been suggested as a primary mechanism of unconsciousness. By using a novel combination of positron emission tomography and functional magnetic resonance imaging, we studied anesthesia-induced unconsciousness and recovery using the α2-agonist dexmedetomidine. During unconsciousness, ...
Bergersen Linda Hildegard LH 1] The Brain and Muscle Energy Group, SN-Lab, Department of Anatomy, Institute of Basic Medical Sciences, Healthy Brain Ageing Centre, University of Oslo, Oslo, Norway [2] Department of Neuroscience and Pharmacology, University of Copenhagen, Copenhagen, Denmark [3] Center for Healthy Aging, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark [4] The Brain and Muscle Energy Group, Department of Oral Biology, University of Oslo, Oslo, - - 2014
Lactate acts as a 'buffer' between glycolysis and oxidative metabolism. In addition to being exchanged as a fuel by the monocarboxylate transporters (MCTs) between cells and tissues with different glycolytic and oxidative rates, lactate may be a 'volume transmitter' of brain signals. According to some, lactate is a preferred fuel ...
Jones Amanda S AS Haematology Department, Camelia Botnar Laboratories, Great Ormond Street Hospital for Children, NHS Foundation Trust, London, - - 2014
Historically, haematology analyser flags for abnormal white blood cells (WBCs) show good sensitivity but lower specificity, causing unnecessary blood film reviews. While the WBC differential channel on Sysmex XE and XN instruments reports a combined flag for blasts/abnormal lymphocytes, the new white precursor cell channel (WPC) on the XN series ...
Ahluwalia Balpreet Singh BS Department of Physics and Technology, University of Tromsø, N-9037 Tromsø, Norway. - - 2014
Red blood cells squeeze through micro-capillaries as part of blood circulation in the body. The deformability of red blood cells is thus critical for blood circulation. In this work, we report a method to optically squeeze red blood cells using the evanescent field present on top of a planar waveguide ...
Warnke Clemens C Department of Neurology, Medical Faculty, Heinrich-Heine-University, Duesseldorf, Germany - - 2014
Natalizumab for multiple sclerosis (MS) increases the risk of progressive multifocal leukoencephalopathy (PML). We aimed to assess the effect of natalizumab on cellular composition and functional B cell parameters including patients with natalizumab-associated PML (n=37). Cellular composition by flow cytometry, levels of immunoglobulin (Ig)G/IgM by immunonephelometry, and oligoclonal bands by ...
Huang Sha S Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA, USA. - - 2014
In this study, the effects of prolonged storage on several biophysical properties of red blood cells (RBCs) were investigated. Single cell deformability was used as an important criterion in determining subgroups of RBCs evolved during storage lesion. A deformability-based microfluidic cell sorting technology was applied, which demonstrates the ability to ...
DiNuzzo Mauro M Enrico Fermi Center - - 2014
Extensive research over the past decades about the multifaceted roles of brain astrocytes led to the suggestion that the signals observed with functional neuroimaging might primarily reflect astrocytic rather than neuronal activity. The basis for this paradigm-shifting concept was the evidence for an involvement of astrocytes in the control of ...
Pries Axel R AR Department of Physiology and CCR, Charité, Berlin, Germany; Deutsches Herzzentrum Berlin, Berlin, Germany; - - 2014
The adequate and efficient functioning of the microcirculation requires not only numerous vessels providing a large surface area for transport but also a structure that provides short diffusion distances from capillaries to tissue and efficient distribution of convective blood flow. Theoretical models show how a combination of angiogenesis, remodeling, and ...
Gonzalez-Escamilla Gabriel G Laboratory of Functional Neuroscience, Spanish Network of Excellence for Research on Neurodegenerative Diseases (CIBERNED), Pablo de Olavide University, Ctra. de Utrera Km 1, 41013, Seville, - - 2014
Growing evidence suggests that decreased functional connectivity in cortical networks precedes clinical stages of Alzheimer's disease (AD), although our knowledge about cerebral and biological correlates of this phenomenon is limited. To shed light on this issue, we have investigated whether resting-state oscillatory connectivity patterns in healthy older (HO) and amnestic ...
Baker Wesley B WB Dept. Physics and Astronomy, University of Pennsylvania, Philadelphia, PA 19104, - - 2014
We develop and validate a Modified Beer-Lambert law for blood flow based on diffuse correlation spectroscopy (DCS) measurements. The new formulation enables blood flow monitoring from temporal intensity autocorrelation function data taken at single or multiple delay-times. Consequentially, the speed of the optical blood flow measurement can be substantially increased. ...
Stroot Peter G PG Western Autotroph Company LLC, Parker, CO 80134, United States. Electronic address: - - 2014
The inorganic sulfur content of the Western Diet is more than six times greater than the minimal daily requirement. The non-adsorbed organic and inorganic sulfur supports high levels of sulfate reducing bacteria (SRB) in the large intestine. Over time, the combination of elevated dietary inorganic sulfur and high levels of ...
Grau Marijke M Institute of Cardiovascular Research and Sport Medicine, Department of Molecular and Cellular Sport Medicine, German Sport University Cologne, Cologne, Germany; The German Research Centre of Elite Sport, German Sport University Cologne, Cologne, - - 2014
Human red blood cells (RBC) express an active and functional endothelial-like nitric oxide (NO) synthase (RBC-NOS). We report studies on RBC-NOS activity in sickle cell anaemia (SCA), a genetic disease characterized by decreased RBC deformability and vascular dysfunction. Total RBC-NOS content was not significantly different in SCA patients compared to ...
Machnes-Maayan Diti D Pediatric Department B, Pediatric Immunology Service, Jeffrey Modell Foundation (JMF) Center, Edmond and Lily Safra Children's Hospital, Chaim Sheba Medical Center, 52621, Tel Hashomer, - - 2014
The thymus is a highly specialized organ for T cell receptor (TCR) rearrangement and selection mechanisms that ensure the formation of functional and self-tolerant cells. Little is known about how peripheral blood assessment of thymic function reflects thymus activity during infancy. We compared thymic function-related markers in the thymus with ...
Ni Chen C Key Laboratory of Protein and Peptide Pharmaceuticals, Chinese Academy of Sciences-University of Tokyo Joint Laboratory of Structural Virology and Immunology, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China; University of the Chinese Academy of Sciences, Beijing, - - 2014
The function of blood-brain barrier is often disrupted during the progression of multiple sclerosis and its animal model, experimental autoimmune encephalomyelitis (EAE). However, the molecular mechanism of blood-brain barrier modulation during neuroinflammation remains unclear. Herein, we show that the expression of interferon-γ (IFNγ) receptor on endothelial cells (ECs) protected mice ...
Patibandla Phani K - - 2014
Type 2 diabetes significantly elevates risk of cardiovascular disease. This can be largely attributed to the adverse effects of hyperglycemic conditions on normal endothelial cell (EC) function. ECs in both large and small vessels are influenced by hyperglycemic conditions, which increase susceptibility to EC dysfunction and atherosclerotic lesion formation. Fluid ...
Wiencke John K JK a Department of Neurological Surgery ; University of California, San Francisco ; San Francisco , CA - - 2014
Quantitating the copy number of demethylated CpG promoter sites of the CD3Z gene can be used to estimate the numbers and proportions of T cells in human blood and tissue. Quantitative methylation specific PCR (qPCR) is useful for studying T cells but requires extensive calibration and is imprecise at low ...
Li Xiang X Integrated Biosystems and Biomechanics Laboratory, University of Michigan, Ann Arbor, MI 48109, USA; Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, - - 2014
The available techniques for assessing blood cell functions are limited considering the various types of blood cell and their diverse functions. In the past decade, rapid advances in microengineering have enabled an array of blood cell functional measurements that are difficult or impossible to achieve using conventional bulk platforms. Such ...
Li Ling L Department of Transfusion Medicine, Shaanxi Provincial People's Hospital, Xi'an 710068, - - 2014
This study aimed to determine the optimal composition and timing for the administration of blood supplements during in vivo blood transfusion with red blood cells suspension (pRBC), fresh frozen plasma (FFP), and apheresis platelet (PLT) administered for the correction of anemia and coagulation dysfunction caused by in vitro hemodilution. We ...
Mezue Melvin M Nuffield Department of Clinical Neurosciences, Oxford Centre for Functional Magnetic Resonance Imaging of the Brain (FMRIB), University of Oxford, Oxford, - - 2014
Arterial spin labeling (ASL) sequences that incorporate multiple postlabeling delay (PLD) times allow estimation of when arterial blood signal arrives within a region of interest. Sequences that account for such variability may improve the reliability of ASL and therefore make the technique well suited for future clinical and experimental investigations ...
Loeken Mary R MR Section on Islet and Regenerative Cell Biology, Joslin Diabetes Center, - - 2014
There are currently few solutions for diabetic vascular disease that involve repair of damaged tissues. The manuscript by Porat et al. in this issue, suggests a possible method to use a patient's own circulating blood cells to provide progenitors to repair damaged vascular tissues. Copyright © 2014 John Wiley & ...
Santisakultarm Thom P TP Department of Biomedical Engineering, Cornell University, Ithaca, NY; National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, - - 2014
Essential thrombocythemia (ET) and polycythemia vera (PV) are myeloproliferative neoplasms (MPNs) that share the JAK2(V617F) mutation in hematopoietic stem cells, leading to excessive production of predominantly platelets in ET, and predominantly red blood cells (RBCs) in PV. The major cause of morbidity and mortality in PV and ET is thrombosis, ...
Feng Ru R Department of Materials Science and Engineering, Jinan University, 510632, - - 2014
Gaphene and its derivatives have become great concern in biomedical fields. Though many investigations about their toxicity have been reported, systematic investigation on the interaction with multiple blood components is lacking. In this work, we studied the effects of the graphene oxide (GO) on the structure and function of the ...
Liu Shiping S Department of Entomology, The State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, - - 2014
Lineage-specific microRNAs (miRNAs) may contribute to functions specific to hematophagous mosquitoes and, as such, have potential for contributing to the development of future mosquito control approaches. Here we report that the mosquito- and gut-specific miRNA, miR-1174, is required for proper sugar absorption, fluid excretion, blood intake, and, consequently, egg maturation ...
Engels Gerwin E GE HaemoScan BV, Groningen - The - - 2014
Cardiopulmonary bypass is still a major cause of lung injury and delay in pulmonary recovery after cardiac surgery. Although it has been shown that pulsatile flow induced by intra-aortic balloon pumping is beneficial for preserving lung function, it is not clear if the same beneficial effect can be accomplished with ...
Benedik Penelope S PS Department of Acute and Continuing Care, School of Nursing, University of Texas Health Science Center at Houston, 6901 Bertner Street, SON 682, Houston, TX 77030, USA. Electronic address: - - 2014
Erythrocytes are not just oxygen delivery devices but play an active metabolic role in modulating microvascular blood flow. Hemoglobin and red blood cell morphology change as local oxygen levels fall, eliciting the release of adenosine triphosphate and nitric oxide to initiate local vasodilation. Aged erythrocytes undergo physical and functional changes ...
Kotan Dilcan D Department of Neurology, Faculty of Medicine, Sakarya University, - - 2014
The choroid plexuses (CPs) are brain structures located in the brain ventricles, involved in the production and reabsorption of cerebrospinal fluid (CSF) components, cerebral immune surveillance, and various endocrine-enzymatic activities and acts as a CSF-blood barrier. This study investigated to determine if there is a link between ischemic CP injury and meningo-cerebral ...
Richard Jennings J J Department of Psychiatry, University of Pittsburgh, Pittsburgh, Pennsylvania, USA; Department of Psychology, University of Pittsburgh, Pittsburgh, Pennsylvania, - - 2014
The neurovisceral integration hypothesis suggests in part that cerebral control of autonomic function conveys comparable control of executive function and, hence, correlation among vagally determined high frequency heart rate variability (HF-HRV), executive function, and regional cerebral blood flow (CBF). In 440 middle-aged men and women, resting HF-HRV was related to ...
Berger Terry A TA SFC Solutions, Inc., 9435 Downing St, Englewood, FL 34224, United States. Electronic address: - - 2014
A mis-match between the post-column mobile phase temperature and the UV detector flow cell temperature can cause significant UV noise in supercritical fluid chromatography (SFC). Deviations as little as 5°C can increase noise as much as 5 times, making the detector unsuited for trace analysis. Two approaches were used to ...
Cheng Ying Y F. M. Kirby Research Center for Functional Brain Imaging, Kennedy Krieger Institute, Baltimore, MD, USA; Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, - - 2014
In addition to the BOLD scan, quantitative functional MRI studies require measurement of both cerebral blood volume (CBV) and flow (CBF) dynamics. The ability to detect CBV and CBF responses in a single additional scan would shorten the total scan time and reduce temporal variations. Several approaches for simultaneous CBV ...
Lai Enyin E Zheijiang - - 2014
Pancreatic islet blood perfusion varies according to the needs for insulin secretion. We examined the effects of blood lipids on pancreatic islet blood flow in anesthetized rats. Acute administration of intralipid to anesthetized rats increased both triglycerides and free fatty acids, associated with a simultaneous increase in total pancreatic and ...
Carver Koryn A KA Cardiovascular Research Center, Medical College of Wisconsin, Milwaukee, Wisconsin; Department of Physiology, Medical College of Wisconsin, Milwaukee, Wisconsin; - - 2014
Mammals have circadian variation in blood pressure, heart rate, vascular tone, thrombotic tendency, and cerebral blood flow (CBF). These changes may be in part orchestrated by circadian variation in clock gene expression within cells comprising the vasculature that modulate blood flow (e.g., fibroblasts, cerebral vascular smooth muscle cells, astrocytes, and ...
Azzolini Michele M Dept. Biomedical Sciences, University of Padova, Viale G. Colombo 3, 35121, Padova, - - 2014
Pterostilbene (Pt) is emerging as an important health-promoting natural compound. Pharmacokinetic studies so far have focused on plasma levels, while Pt distribution in tissues is most relevant for biological action. This study determined tissue distribution of Pt and its major metabolite, pterostilbene-4'-sulfate (Pt-S), in rats after oral administration. Upon intravenous ...
Fujii Shinobu S Research and Development Center, Santen Pharmaceutical Co., Ltd., Takayama-cho, 8916-16, Ikoma-shi, Nara, 6300101, Japan - - 2014
Purpose: To clarify the impact of P-glycoprotein (P-gp) on blood-retinal barrier (BRB) and blood-aqueous humor barrier (BAB) permeability, in contrast to blood-brain barrier (BBB) permeability. Methods: Permeabilities of six compounds, including P-gp substrates (quinidine, digoxin, and verapamil), were investigated in wild-type and mdr1a knockout rats using retinal, aqueous humor, and ...
Bart Thomas T Swiss Transfusion SRC, Bern, - - 2014
To obtain a better understanding of factors affecting blood and blood stem cell donation behavior in Switzerland, a series of studies has been performed. In the recent study of this series, which is described here, motivators and barriers in the field of blood and blood stem cell donation were identified. ...
Zhang Jing J Affiliated Hospital of the Academy of Military Medical Sciences, Beijing 100071, - - 2014
The main purpose of the present study was to examine the time-dependent alterations in the endothelial cell density that occur in the first 180 d after irradiation of the spinal cord and the functional role of these alterations in the spinal cord blood flow. An irradiated cervical spinal cord rat ...
Helms Hans Christian HC Department of Pharmacy, The Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, 2100, Copenhagen, - - 2014
Efflux transporters of the ATP-binding cassette superfamily including breast cancer resistance protein (Bcrp/Abcg2), P-glycoprotein (P-gp/Abcb1) and multidrug resistance-associated proteins (Mrp's/Abcc's) are expressed in the blood-brain barrier (BBB). The aim of this study was to investigate if a bovine endothelial/rat astrocyte in vitro BBB co-culture model displayed polarized transport of known ...
Lanz Bernard B Laboratory for Functional and Metabolic Imaging (LIFMET), Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland - - 2014
Measurement of arterial input function is a restrictive aspect for quantitative (18)F-FDG PET studies in rodents because of their small total blood volume and the related difficulties in withdrawing blood. In the present study, we took advantage of the high spatial resolution of a recent dedicated small-animal scanner to extract ...
Matheson Paul J PJ Robley Rex Veterans Affairs Medical Center at Louisville, KY; Hiram C. Polk, Jr., M.D. Department of Surgery, University of Louisville, Louisville, - - 2014
Intestinal vasoconstriction is a critical step in development of necrotizing enterocolitis (NEC). Relaxin (RLXN), a hormone found in breast milk but absent from formula, is a potent vasodilator. We hypothesized that relaxin-supplemented feeds with an NEC protocol would decrease NEC severity and increase intestinal blood flow. Timed-pregnant Sprague-Dawley rats were ...
Wiczling Paweł P Department of Biopharmaceutics and Pharmacodynamics, Medical University of Gdańsk, Gdańsk, - - 2014
A technique has recently been proposed for obtaining the reticulocyte (RET) age distribution from flow cytometric reticulocyte count. It allows for a quantitative characterization of reticulocytes dynamics. In this work this technique was applied to characterize the blood, bone marrow and spleen reticulocytes in homeostatic and erythropoietically stimulated rats in ...
Tarhouni Kahena K From the LUNAM University and University of Angers, Angers, France (K.T., B.T., V.P., L.G., D.H.); CNRS UMR 6214, Angers, France (A.L.G., L.L., D.H.); INSERM U1083, Angers, France (D.H.); CHU d'Angers, Angers, France (E.V., V.P., D.H.); and INSERM U1048, Toulouse III Paul Sabatier University, CHU de Toulouse, Toulouse, France (F.L., - - 2014
Flow (shear stress)-mediated outward remodeling (FMR) of resistance arteries is a key adaptive process allowing collateral growth after arterial occlusion but declining with age. 17-β-estradiol (E2) has a key role in this process through activation of estrogen receptor α (ERα). Thus, we investigated the impact of age and timing for ...
Murakami Eduardo E University of Franca, Franca, SP, - - 2014
To evaluate the female sterilization by occlusion of the ovarian blood flow, using the rat as experimental model. Fifty-five females rats were divided into four groups: I (n=10), bilateral ovariectomy, euthanized at 60 or 90 days; II (n=5), opening the abdominal cavity, euthanized at 90 days; III (n=20), bilateral occlusion ...
Shi Xuqin X Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engeering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, - - 2014
Blood deficiency is commonly encountered among women, and is the root of many gynaecological disorders. Danggui Buxue Decoction (DBD), a classical traditional Chinese formula which is composed of Astragali Radix (AR) and Angelicae Sinensis Radix (ASR) at the ratio of 5:1 (w/w), is widely used in TCM clinics for treatment ...
Kasamoto Sawako S Public Interest Incorporated Foundation, BioSafety Research Center (BSRC), 582-2, Shioshinden, Iwata, Shizuoka 437-1213, Japan. Electronic address: - - 2014
In anticipation of proposed OECD guideline changes that may include increasing the number of reticulocytes scored for micronuclei, an inter-laboratory reproducibility study of the rat peripheral blood micronucleus assay was performed using flow cytometry. In this experiment, male Sprague-Dawley (SD) rats were treated with the model clastogen cyclophosphamide (CP: 5, ...
Isaksson K K Department of Surgery, Skåne University Hospital in Lund, Getingevägen 4, 221 85, Lund, - - 2014
The combination of two differently charged polypeptides, poly-L-lysine (PL) and poly-L-glutamate (PG), has shown excellent postsurgical antiadhesive properties. However, the high molecular, positively charged PL is toxic in high doses, proposed as lysis of red blood cells. This study aims to elucidate the in vivo toxicity and biodistribution of PL ...
Qin Xiao-Ling XL Laboratory of Drug Metabolism and Pharmacokinetics, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, China; Guangdong Food and Drug Vocational College, Guangzhou, - - 2014
We recently reported that Wuzhi tablet (WZ), a preparation of the ethanol extract of Wuweizi (Schisandra sphenanthera), had significant effects on blood concentrations of Tacrolimus (FK506) in renal transplant recipients and rats. The active lignans in WZ are schisandrin A, schisandrin B, schisandrin C, schisandrol A, schisandrol B, schisantherin A, ...
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