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Grau Marijke M Institute of Cardiovascular Research and Sport Medicine, Department of Molecular and Cellular Sport Medicine, German Sport University Cologne, Cologne, Germany; The German Research Centre of Elite Sport, German Sport University Cologne, Cologne, - - 2014
Human red blood cells (RBC) express an active and functional endothelial-like nitric oxide (NO) synthase (RBC-NOS). We report studies on RBC-NOS activity in sickle cell anaemia (SCA), a genetic disease characterized by decreased RBC deformability and vascular dysfunction. Total RBC-NOS content was not significantly different in SCA patients compared to ...
Machnes-Maayan Diti D Pediatric Department B, Pediatric Immunology Service, Jeffrey Modell Foundation (JMF) Center, Edmond and Lily Safra Children's Hospital, Chaim Sheba Medical Center, 52621, Tel Hashomer, - - 2014
The thymus is a highly specialized organ for T cell receptor (TCR) rearrangement and selection mechanisms that ensure the formation of functional and self-tolerant cells. Little is known about how peripheral blood assessment of thymic function reflects thymus activity during infancy. We compared thymic function-related markers in the thymus with ...
Ni Chen C Key Laboratory of Protein and Peptide Pharmaceuticals, Chinese Academy of Sciences-University of Tokyo Joint Laboratory of Structural Virology and Immunology, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China; University of the Chinese Academy of Sciences, Beijing, - - 2014
The function of blood-brain barrier is often disrupted during the progression of multiple sclerosis and its animal model, experimental autoimmune encephalomyelitis (EAE). However, the molecular mechanism of blood-brain barrier modulation during neuroinflammation remains unclear. Herein, we show that the expression of interferon-γ (IFNγ) receptor on endothelial cells (ECs) protected mice ...
Patibandla Phani K - - 2014
Type 2 diabetes significantly elevates risk of cardiovascular disease. This can be largely attributed to the adverse effects of hyperglycemic conditions on normal endothelial cell (EC) function. ECs in both large and small vessels are influenced by hyperglycemic conditions, which increase susceptibility to EC dysfunction and atherosclerotic lesion formation. Fluid ...
Li Xiang X Integrated Biosystems and Biomechanics Laboratory, University of Michigan, Ann Arbor, MI 48109, USA; Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, - - 2014
The available techniques for assessing blood cell functions are limited considering the various types of blood cell and their diverse functions. In the past decade, rapid advances in microengineering have enabled an array of blood cell functional measurements that are difficult or impossible to achieve using conventional bulk platforms. Such ...
Li Ling L Department of Transfusion Medicine, Shaanxi Provincial People's Hospital, Xi'an 710068, - - 2014
This study aimed to determine the optimal composition and timing for the administration of blood supplements during in vivo blood transfusion with red blood cells suspension (pRBC), fresh frozen plasma (FFP), and apheresis platelet (PLT) administered for the correction of anemia and coagulation dysfunction caused by in vitro hemodilution. We ...
Mezue Melvin M Nuffield Department of Clinical Neurosciences, Oxford Centre for Functional Magnetic Resonance Imaging of the Brain (FMRIB), University of Oxford, Oxford, - - 2014
Arterial spin labeling (ASL) sequences that incorporate multiple postlabeling delay (PLD) times allow estimation of when arterial blood signal arrives within a region of interest. Sequences that account for such variability may improve the reliability of ASL and therefore make the technique well suited for future clinical and experimental investigations ...
Loeken Mary R MR Section on Islet and Regenerative Cell Biology, Joslin Diabetes Center, - - 2014
There are currently few solutions for diabetic vascular disease that involve repair of damaged tissues. The manuscript by Porat et al. in this issue, suggests a possible method to use a patient's own circulating blood cells to provide progenitors to repair damaged vascular tissues. Copyright © 2014 John Wiley & ...
Santisakultarm Thom P TP Department of Biomedical Engineering, Cornell University, Ithaca, NY; National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, - - 2014
Essential thrombocythemia (ET) and polycythemia vera (PV) are myeloproliferative neoplasms (MPNs) that share the JAK2(V617F) mutation in hematopoietic stem cells, leading to excessive production of predominantly platelets in ET, and predominantly red blood cells (RBCs) in PV. The major cause of morbidity and mortality in PV and ET is thrombosis, ...
Feng Ru R Department of Materials Science and Engineering, Jinan University, 510632, - - 2014
Gaphene and its derivatives have become great concern in biomedical fields. Though many investigations about their toxicity have been reported, systematic investigation on the interaction with multiple blood components is lacking. In this work, we studied the effects of the graphene oxide (GO) on the structure and function of the ...
Liu Shiping S Department of Entomology, The State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, - - 2014
Lineage-specific microRNAs (miRNAs) may contribute to functions specific to hematophagous mosquitoes and, as such, have potential for contributing to the development of future mosquito control approaches. Here we report that the mosquito- and gut-specific miRNA, miR-1174, is required for proper sugar absorption, fluid excretion, blood intake, and, consequently, egg maturation ...
Engels Gerwin E GE HaemoScan BV, Groningen - The - - 2014
Cardiopulmonary bypass is still a major cause of lung injury and delay in pulmonary recovery after cardiac surgery. Although it has been shown that pulsatile flow induced by intra-aortic balloon pumping is beneficial for preserving lung function, it is not clear if the same beneficial effect can be accomplished with ...
Benedik Penelope S PS Department of Acute and Continuing Care, School of Nursing, University of Texas Health Science Center at Houston, 6901 Bertner Street, SON 682, Houston, TX 77030, USA. Electronic address: - - 2014
Erythrocytes are not just oxygen delivery devices but play an active metabolic role in modulating microvascular blood flow. Hemoglobin and red blood cell morphology change as local oxygen levels fall, eliciting the release of adenosine triphosphate and nitric oxide to initiate local vasodilation. Aged erythrocytes undergo physical and functional changes ...
Richard Jennings J J Department of Psychiatry, University of Pittsburgh, Pittsburgh, Pennsylvania, USA; Department of Psychology, University of Pittsburgh, Pittsburgh, Pennsylvania, - - 2014
The neurovisceral integration hypothesis suggests in part that cerebral control of autonomic function conveys comparable control of executive function and, hence, correlation among vagally determined high frequency heart rate variability (HF-HRV), executive function, and regional cerebral blood flow (CBF). In 440 middle-aged men and women, resting HF-HRV was related to ...
Berger Terry A TA SFC Solutions, Inc., 9435 Downing St, Englewood, FL 34224, United States. Electronic address: - - 2014
A mis-match between the post-column mobile phase temperature and the UV detector flow cell temperature can cause significant UV noise in supercritical fluid chromatography (SFC). Deviations as little as 5°C can increase noise as much as 5 times, making the detector unsuited for trace analysis. Two approaches were used to ...
Cheng Ying Y Division of MR Research, Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins University School of Medicine, Baltimore, MD, USA.; Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, - - 2014
In addition to the BOLD scan, quantitative functional MRI studies require measurement of both cerebral blood volume (CBV) and flow (CBF) dynamics. The ability to detect CBV and CBF responses in a single additional scan would shorten the total scan time and reduce temporal variations. Several approaches for simultaneous CBV ...
Lai Enyin E Zheijiang - - 2014
Pancreatic islet blood perfusion varies according to the needs for insulin secretion. We examined the effects of blood lipids on pancreatic islet blood flow in anesthetized rats. Acute administration of intralipid to anesthetized rats increased both triglycerides and free fatty acids, associated with a simultaneous increase in total pancreatic and ...
Houdek Koryn K Medical College of - - 2014
Mammals have circadian variation in blood pressure, heart rate, vascular tone, thrombotic tendency and cerebral blood flow (CBF). These changes may be in part orchestrated by circadian variation in clock gene expression within cells comprising the vasculature that modulate blood flow (e.g., fibroblasts, cerebral vascular smooth muscle cells , astrocytes ...
Azzolini Michele M Dept. Biomedical Sciences, University of Padova, Viale G. Colombo 3, 35121, Padova, - - 2014
Pterostilbene (Pt) is emerging as an important health-promoting natural compound. Pharmacokinetic studies so far have focused on plasma levels, while Pt distribution in tissues is most relevant for biological action. This study determined tissue distribution of Pt and its major metabolite, pterostilbene-4'-sulfate (Pt-S), in rats after oral administration. Upon intravenous ...
Fujii Shinobu S Research and Development Center, Santen Pharmaceutical Co., Ltd., Takayama-cho, 8916-16, Ikoma-shi, Nara, 6300101, Japan - - 2014
Purpose: To clarify the impact of P-glycoprotein (P-gp) on blood-retinal barrier (BRB) and blood-aqueous humor barrier (BAB) permeability, in contrast to blood-brain barrier (BBB) permeability. Methods: Permeabilities of six compounds, including P-gp substrates (quinidine, digoxin, and verapamil), were investigated in wild-type and mdr1a knockout rats using retinal, aqueous humor, and ...
Bart Thomas T Swiss Transfusion SRC, Bern, - - 2014
To obtain a better understanding of factors affecting blood and blood stem cell donation behavior in Switzerland, a series of studies has been performed. In the recent study of this series, which is described here, motivators and barriers in the field of blood and blood stem cell donation were identified. ...
Zhang Jing J Affiliated Hospital of the Academy of Military Medical Sciences, Beijing 100071, - - 2014
The main purpose of the present study was to examine the time-dependent alterations in the endothelial cell density that occur in the first 180 d after irradiation of the spinal cord and the functional role of these alterations in the spinal cord blood flow. An irradiated cervical spinal cord rat ...
Helms Hans Christian HC Department of Pharmacy, The Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, 2100, Copenhagen, - - 2014
Efflux transporters of the ATP-binding cassette superfamily including breast cancer resistance protein (Bcrp/Abcg2), P-glycoprotein (P-gp/Abcb1) and multidrug resistance-associated proteins (Mrp's/Abcc's) are expressed in the blood-brain barrier (BBB). The aim of this study was to investigate if a bovine endothelial/rat astrocyte in vitro BBB co-culture model displayed polarized transport of known ...
Lanz Bernard B Laboratory for Functional and Metabolic Imaging (LIFMET), Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland - - 2014
Measurement of arterial input function is a restrictive aspect for quantitative (18)F-FDG PET studies in rodents because of their small total blood volume and the related difficulties in withdrawing blood. In the present study, we took advantage of the high spatial resolution of a recent dedicated small-animal scanner to extract ...
Matheson Paul J PJ Robley Rex Veterans Affairs Medical Center at Louisville, KY; Hiram C. Polk, Jr., M.D. Department of Surgery, University of Louisville, Louisville, - - 2014
Intestinal vasoconstriction is a critical step in development of necrotizing enterocolitis (NEC). Relaxin (RLXN), a hormone found in breast milk but absent from formula, is a potent vasodilator. We hypothesized that relaxin-supplemented feeds with an NEC protocol would decrease NEC severity and increase intestinal blood flow. Timed-pregnant Sprague-Dawley rats were ...
Wiczling Paweł P Department of Biopharmaceutics and Pharmacodynamics, Medical University of Gdańsk, Gdańsk, - - 2014
A technique has recently been proposed for obtaining the reticulocyte (RET) age distribution from flow cytometric reticulocyte count. It allows for a quantitative characterization of reticulocytes dynamics. In this work this technique was applied to characterize the blood, bone marrow and spleen reticulocytes in homeostatic and erythropoietically stimulated rats in ...
Tarhouni Kahena K From the LUNAM University and University of Angers, Angers, France (K.T., B.T., V.P., L.G., D.H.); CNRS UMR 6214, Angers, France (A.L.G., L.L., D.H.); INSERM U1083, Angers, France (D.H.); CHU d'Angers, Angers, France (E.V., V.P., D.H.); and INSERM U1048, Toulouse III Paul Sabatier University, CHU de Toulouse, Toulouse, France (F.L., - - 2014
Flow (shear stress)-mediated outward remodeling (FMR) of resistance arteries is a key adaptive process allowing collateral growth after arterial occlusion but declining with age. 17-β-estradiol (E2) has a key role in this process through activation of estrogen receptor α (ERα). Thus, we investigated the impact of age and timing for ...
Murakami Eduardo E University of Franca, Franca, SP, - - 2014
To evaluate the female sterilization by occlusion of the ovarian blood flow, using the rat as experimental model. Fifty-five females rats were divided into four groups: I (n=10), bilateral ovariectomy, euthanized at 60 or 90 days; II (n=5), opening the abdominal cavity, euthanized at 90 days; III (n=20), bilateral occlusion ...
Shi Xuqin X Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engeering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, - - 2014
Blood deficiency is commonly encountered among women, and is the root of many gynaecological disorders. Danggui Buxue Decoction (DBD), a classical traditional Chinese formula which is composed of Astragali Radix (AR) and Angelicae Sinensis Radix (ASR) at the ratio of 5:1 (w/w), is widely used in TCM clinics for treatment ...
Kasamoto Sawako S Public Interest Incorporated Foundation, BioSafety Research Center (BSRC), 582-2, Shioshinden, Iwata, Shizuoka 437-1213, Japan. Electronic address: - - 2014
In anticipation of proposed OECD guideline changes that may include increasing the number of reticulocytes scored for micronuclei, an inter-laboratory reproducibility study of the rat peripheral blood micronucleus assay was performed using flow cytometry. In this experiment, male Sprague-Dawley (SD) rats were treated with the model clastogen cyclophosphamide (CP: 5, ...
Isaksson K K Department of Surgery, Skåne University Hospital in Lund, Getingevägen 4, 221 85, Lund, - - 2014
The combination of two differently charged polypeptides, poly-L-lysine (PL) and poly-L-glutamate (PG), has shown excellent postsurgical antiadhesive properties. However, the high molecular, positively charged PL is toxic in high doses, proposed as lysis of red blood cells. This study aims to elucidate the in vivo toxicity and biodistribution of PL ...
Qin Xiao-Ling XL Laboratory of Drug Metabolism and Pharmacokinetics, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, China; Guangdong Food and Drug Vocational College, Guangzhou, - - 2014
We recently reported that Wuzhi tablet (WZ), a preparation of the ethanol extract of Wuweizi (Schisandra sphenanthera), had significant effects on blood concentrations of Tacrolimus (FK506) in renal transplant recipients and rats. The active lignans in WZ are schisandrin A, schisandrin B, schisandrin C, schisandrol A, schisandrol B, schisantherin A, ...
van Gestel Margriet A MA Brain Center Rudolf Magnus, Department of Translational Neuroscience, University Medical Center Utrecht, University of Utrecht, Utrecht, The - - 2014
To promote the efficient and safe application of adeno-associated virus (AAV) vectors as a gene transfer tool in the central nervous system (CNS), transduction efficiency and clearance were studied for serotypes commonly used to transfect distinct areas of the brain. As AAV2 was shown to transduce only small volumes in ...
Morshed Khandakar Niaz KN Department of Mechanical Engineering, Colorado State University, Fort Collins, Colorado, United States of - - 2014
Shear stress on blood cells and platelets transported in a turbulent flow dictates the fate and biological activity of these cells. We present a theoretical link between energy dissipation in turbulent flows to the shear stress that cells experience and show that for the case of physiological turbulent blood flow: ...
Wu Meiye M Biological Science and Technology, Sandia National Laboratories, MS 9292, 969, Livermore, CA, 94551-0969, - - 2014
Flow cytometry in combination with fluorescent in situ hybridization (flow-FISH) is a powerful technique that can be utilized to rapidly detect nucleic acids at single-cell resolution without the need for homogenization or nucleic acid extraction. Here, we describe a microfluidic-based method which enables the detection of microRNAs or miRNAs in ...
Bai Long L Department of Mechanics, Tianjin University, Tianjin, - - 2014
The mechanical behavior of blood cells in the vessels has a close relationship with the physical characteristics of the blood and the cells. In this paper, a numerical simulation method was proposed to understand a single-blood cell's behavior in the vessels based on fluid-solid interaction method, which was conducted under ...
Du Li L Department of Anesthesiology, Sichuan Cancer Hospital No. 55, People's South Road, Chengdu 610041, Sichuan, - - 2014
A 16-year-old male with adolescent idiopathic scoliosis was scheduled for the posterior spinal fusion (PSF) under general anesthesia. The cell saver was routinely prepared preoperatively and 400 ml of salvaged red blood cells were transfused during the surgery. After the cell salvage transfusion, the oozing of blood in surgical wounds ...
Chou Chih-Wei CW Department of Life Science, Tunghai University, Taichung, - - 2014
While the endothelium-organ interaction is critical for regulating cellular behaviors during development and disease, the role of blood flow in these processes is only partially understood. The dorsal aorta performs paracrine functions for the timely migration and differentiation of the sympatho-adrenal system. However, it is unclear how the adrenal cortex ...
Ayers Lisa L Department of Clinical Immunology, Oxford University Hospitals NHS Trust, Oxford, - - 2014
Flow cytometry is the most commonly used technology to measure microvesicles (MVs). Despite reported limitations of this technique, MV levels obtained using conventional flow cytometry have yielded many clinically relevant findings, such as associations with disease severity and ability to predict clinical outcomes. This study aims to determine if MV ...
- - 2014
[This corrects the article DOI: 10.1371/journal.pone.0102707.].
Zhang Congcong C School of Information Science and Engineering, Shandong University, Jinan 250100, China. - - 2014
White blood cell (WBC) segmentation, which is important for cytometry, is a challenging issue because of the morphological diversity of WBCs and the complex and uncertain background of blood smear images. This paper proposes a novel method for the nucleus and cytoplasm segmentation of WBCs for cytometry. A color adjustment ...
Schrödl Falk F Department of Ophthalmology, Paracelsus Medical University, SALK, Muellner Hauptstrasse 48, 5020, Salzburg, Austria, - - 2013
Pericytes are contractile cells that surround blood vessels. When contracting, they change the diameter of the vessel and therefore influence blood flow homeostasis; however, mechanisms controlling pericyte action are less well understood. Since blood flow regulation per se is controlled by the autonomic nervous system, the latter might also be ...
Song Kimberly J - - 2013
Following trauma, transfusion of aged stored blood is often necessary yet associated with increased morbidity and mortality. Despite blood replacement, many patients have a prolonged anemia requiring further transfusions. The effects of aged blood on bone marrow (BM) hematopoiesis have not been studied, and we hypothesized that stored blood suppresses ...
Gao Xiang - - 2013
Impaired glucose tolerance and type 2 diabetes in rodents are associated with increased islet blood flow. If this is important for modulation of the endocrine function is at present unknown. We evaluated if glucosamine infusion, which induces peripheral insulin resistance and glucose intolerance, could be used to acutely increase islet ...
Ward Tonya L - - 2013
Background:Milk contains immunological constituents that comprise an edible immune system conveyed from mother to newborn. Soluble Cluster of Differentiation 14 (sCD14) is a protein found in significant quantities in human milk (~8-29 μg/ml). At a tenfold lower concentration in the blood (~3 μg/ml), the most notable role of sCD14 is to sequester ...
Kang Yang Jun - - 2013
In this study, we propose a novel microfluidic biosensor for monitoring hemorheological and hemodynamic properties using an extracorporeal bypass loop. To monitor temporal variations of biophysical properties including viscosity, flow rate, and pressure of rat blood, a complex fluidic network is established by connecting the abdominal artery and jugular vein ...
Garcia-Pinto Angélica B - - 2013
Natural products might alter the labeling of blood constituents with technetium-99m ((99m)Tc) and these results may be correlated with modifications of the shape of the red blood cells (RBC). The biodistribution of radiopharmaceuticals can be also altered. This investigation aimed to determine biological effects of an aqueous extract of chamomile ...
Chuang Chih Hsiang - - 2013
Electroacupuncture (EA) has been widely used for treatment of stroke, but there is little information on the effect of EA on the neuroprotective function in traumatic brain injury (TBI). The aim of the present study was to investigate the protective effects and mechanisms of EA treatment in a TBI rat ...
Stabley John N JN Department of Applied Physiology and Kinesiology, and the Center for Exercise Science, University of Florida, Gainesville, FL 32611, - - 2013
Chronic skeletal unloading diminishes hindlimb bone blood flow. The purpose of the present investigation was to determine 1) whether 7 and 14days of skeletal unloading alter femoral bone and marrow blood flow and vascular resistance during reloading, and 2) whether putative changes in bone perfusion are associated with a gross ...
Shi Xuqin - - 2013
Astragali Radix (AR) and Angelicae Sinensis Radix (ASR) were frequently combined and used in China as herbal pair called as Danggui Buxue Decoction (DBD) for treatment of blood deficiency syndrome, such as women's ailments. This study is to investigate the tissue distribution profiles of five major bio-active constituents (ferulic acid, ...
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