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Results 401 - 450 of 868
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Chen Xigui - - 2003
We investigated sample collection variables that may influence the measurement of the thyroid stimulating hormone (TSH) including: time after birth, season, different ways for blood spot drying and varied elution time from filter paper. TSH was measured with an enzyme-linked immunoassay (EIA) method on dried blood spots collected from newborns ...
Lukacs Z - - 2003
The definitive diagnosis of lysosomal storage disorders depends on the determination of enzymatic activities in cells, tissues or body fluids. At present, neither an evaluation of the different methods nor an interlaboratory quality assurance scheme is available. We have therefore determined the activities of total hexosaminidase, hexosaminidase A and beta-galactosidase ...
Mashange W - - 2003
OBJECTIVES: To validate a Gelatin Particle Agglutination (GPA) test for HIV using dried blood spots with a view to applying the test in large epidemiological studies. DESIGN: A method comparison study with standard enzyme linked immunosorbent assay (ELISA) using the Recombigen HIV-1/2 kit as the gold standard. SETTING: Blair Research ...
Nindl Bradley C - - 2003
Insulin-like growth factor-I (IGF-I) is sensitive to nutritional stress and is reduced in soldiers during stressful field training. Methods have recently been developed to measure IGF-I from filter paper blood spots. Filter paper has advantages over traditional blood sampling in that neither blood separation equipment nor refrigeration is necessary after ...
McDade Thomas W - - 2002
Iron deficiency is the most common micronutrient deficiency worldwide, and transferrin receptor (TfR) level has been identified as an important measure of iron status that is not confounded by inflammation. However, logistical constraints associated with sample collection and processing have limited efforts to measure TfR, particularly at the community level. ...
Jantunen M - - 2002
This review compiles personal and indoor microenvironment particulate matter (PM) monitoring needs from recently set research objectives, most importantly the NRC published "Research Priorities for Airborne Particulate Matter (1998)". Techniques and equipment used to monitor PM personal exposures and microenvironment concentrations and the constituents of the sampled PM during the ...
Posyniak Andrzej - - 2002
A simple, low-cost, sensitive and selective LC method was developed for the determination of enrofloxacin and ciprofloxacin in chicken blood. The method was applied to whole blood from a chicken using dried blood spots on filter paper disks. The detection limits of enrofloxacin and ciprofloxacin (100 microl of whole blood ...
Gallippi Caterina M - - 2002
A method for adaptive clutter rejection via blind source separation (BSS) using principal and independent component analyses is presented in application to blood velocity measurement in the carotid artery. In particular, the filtering method's efficacy for eliminating clutter and preserving lateral blood flow signal components is presented. The performance of ...
Usami Makoto - - 2002
BACKGROUND: The false-positive elevation of plasma (1-->3)-beta-D-glucan level, a serodiagnostic test for deep-seated mycosis, is suspected in patients administered with blood components. STUDY DESIGN AND METHODS: (1-->3)-beta-D-Glucan and endotoxin levels in blood components consisting of 12 albumins, 8 immunoglobulins, and 3 blood coagulation factors were measured by fungal infection tests ...
Katz Russell S - - 2002
The purpose of this study was to evaluate the use of dried blood spots stored on filter paper as a means to provide specimens for virologic surveillance for measles virus (MV) in situations when the reverse cold chain is not available. Two single-step RT-PCR assays were evaluated for sensitivity of ...
Gisolf J - - 2002
Dynamic cerebral autoregulation (CA) has been studied previously using spectral analysis of oscillations in arterial blood pressure (ABP) and cerebral blood flow velocity (CBFV). The dynamics of the CA can be modeled as a high-pass filter. The purpose of this study is to compare CA of blood pressure oscillations induced ...
Shinar Eilat - - 2002
BACKGROUND: Before-storage WBC reduction by filtration appears to be an effective way to prevent transfusion-associated complications. It also has superiority over WBC reduction at the time of transfusion (bedside), due to the many variables associated with such practice and the difficulty in performing adequate quality control. To determine the adaptability ...
Morawska L - - 2002
Despite the electrostatic collection of aerosol particles as one of the most widely used air cleaning methods, there has not been sufficient amount of effort devoted to investigate its performance in the full range of operating conditions. This paper reports results of the tests of a two-stage electrostatic precipitator (ESP) ...
Bjaerum Steinar - - 2002
The quality of ultrasound color flow images is highly dependent on sufficient attenuation of the clutter signals originating from stationary and slowly moving tissue. Without sufficient clutter rejection, the detection of low velocity blood flow will be poor, and the velocity estimates will have a large bias. In some situations, ...
Ichihar Kenichi - - 2002
A convenient method was developed for preparation of FAME in small amounts from glycerolipids of blood or breast milk. Initially, 0.04-0.06 mL blood or breast milk was spotted onto a small piece of filter paper (1.5 x 1.5 cm) that had been washed with acetone containing 0.05% 2,6-di-tert-butyl-p-cresol (BHT). Each ...
Lindmark K - - 2002
Blood cell filterability is an established method in blood rheology. The dynamics at flow onset and its relevance to the data interpretation is, however, not fully known. This paper aims to investigate what controls the length and slope of flow acceleration as the medium accelerates to reach the steady state, ...
Kjeldsen-Kragh J - - 2002
BACKGROUND AND OBJECTIVES: The RCM1 leucocyte-reduction filter has been incorporated into two types of blood-donation sets of which one uses back-priming of the leucocyte filter with SAG-M solution. In this study we have compared the leucocyte-reduction efficacy of the RCM1 filter with or without back priming with SAG-M solution. MATERIALS ...
Morineau-Thomas O - - 2002
Ultrafiltration of two microalgae species, Chorellasp. and Porphyridium purpureum, has been performed in two filtration units of different configurations: a module generating a classical tangential-plane flow and another one inducing a swirling decaying flow. The swirling motion is generated by a unique tangential inlet at the bottom of an annulus. ...
Holland W G - - 2002
In this study we investigated if whole blood could substitute for serum in the direct card agglutination test (CATT/Trypansosoma evansi) and the indirect card agglutination test (LATEX/T. evansi) for the sero-diagnosis of T. evansi in buffaloes. Likewise blood spots on filter paper were compared with sera for use in the ...
Müller-Steinhardt Michael - - 2002
BACKGROUND: The introduction of universal WBC filtration of RBCs prior to storage is currently under consideration in many countries, as it is thought to minimize the incidence of transfusion-associated adverse effects. Centrifugation of blood containers with newly developed soft-shell WBC filters is more convenient, and so of great interest. STUDY ...
Bjaerum Steinar - - 2002
For ultrasound color flow images with high quality, it is important to suppress the clutter signals originating from stationary and slowly moving tissue sufficiently. Without sufficient clutter rejection, low velocity blood flow cannot be measured, and estimates of higher velocities will have a large bias. The small number of samples ...
Seghatchian Jerard - - 2002
Characterisation of the nature of leucodepletion failure, based on the analysis of leucocyte sub-populations, is an essential task for continual improvement in the clinical safety of blood components and for the selection of a safe and appropriate leucodepletion process. Such information is also critical for improving the quality of filters ...
Erhardt Juergen G - - 2002
We describe an improved method for the measurement of retinol in dried blood spots (DBS) on filter paper. Retinol in human DBS on filter paper was analyzed by normal phase HPLC after a simple extraction method. Retinol associated with its binding protein was eluted from the paper into aqueous solution ...
Lee Tzong-Hae - - 2002
BACKGROUND: The effort to eliminate transfusion complications associated with WBCs has led to the widespread use of filters able to reduce WBC concentrations to <or=0.1 WBC per microL blood. This has necessitated sensitive QC methods to quantitate residual WBCs in filtered units. One fast, effective method is DNA amplification using ...
Ramírez Gemma - - 2002
BACKGROUND: Although transfusion or return of salvaged shed blood has become popular in major orthopedic procedures, this blood-saving method is still controversial because shed blood may be contaminated with chemical and tissular debris, such as fat particles, which may increase the risk of fat embolism after bone surgery. STUDY DESIGN ...
Unger J K - - 2002
In Europe, capillary membrane filters are the favored method for plasmapheresis and detoxification systems including a plasma separation unit. Using capillary membrane filters clearance depends on the filtration rate and the sieving coefficient (Qs) for the plasma substrates. We investigated whether the increase of the effective membrane surface of plasmafilters ...
Koehl M A - - 2001
The first step in processing olfactory information, before neural filtering, is the physical capture of odor molecules from the surrounding fluid. Many animals capture odors from turbulent water currents or wind using antennae that bear chemosensory hairs. We used planar laser-induced fluorescence to reveal how lobster olfactory antennules hydrodynamically alter ...
Chiu C C - - 2001
Time domain cross-correlation analysis of pre-filtered mean arterial blood pressure (MABP) and mean cerebral blood flow velocity (MCBFV) was applied to assess the cerebral autoregulation (CA). Beat-to-beat time series of spontaneous arterial blood pressure and cerebral blood flow velocity were obtained from 13 young normal volunteers with the Finapres device ...
Andersson M - - 2001
Asymmetrical flow field-flow fractionation (flow FFF) was connected to multiangle light scattering (MALS) and refractive index (RI) detectors for characterization of the molar mass distribution and molecular radius of a cellulose derivative, ethylhydroxyethyl cellulose (EHEC). Experimental conditions were optimized to allow study of a wide range of molar mass including ...
Jensen U G - - 2001
BACKGROUND: Classic galactosemia (OMIM 230400) is an inherited disorder in the metabolism of galactose caused by deficiency of the enzyme galactose 1-phosphate uridyl transferase (EC 2.7.7.12). Galactosemia leads to accumulation of galactose and galactose 1-phosphate (gal-1-P) in blood and tissues and, if untreated, produces neonatal death or severe mental retardation, ...
Alku P - - 2001
In the production of voiced speech the main excitation of the vocal tract occurs during the glottal closing phase when the rate of change of the flow reaches its absolute maximum. The level of this maximum, the negative peak amplitude of the differentiated glottal flow, correlates strongly with sound pressure ...
Abdulrazzaq Y M - - 2001
OBJECTIVE: To determine whether the well-established filter paper spotted blood method used for the determination of some amino acids could be reliably used to measure all amino acids and whether amino acid results thus obtained are reproducible and comparable to the results obtained by measuring plasma amino acids in either ...
Düngen H D - - 2001
INTRODUCTION: Physical properties of filters for continous renal replacement therapy have a great impact on biocompatibility. According to Poiseuille's law, a filter with more and shorter hollow fibers should offer a decreased pressure drop and, therefore, lower transmembrane pressure (TMP). The aim of this study was to study the effect ...
Iwama H - - 2001
PURPOSE: The purpose of this study was to examine the effect of temperature on bradykinin generation during blood transfusion using positively charged (positive filter), negatively charged (negative filter), and neutral (neutral filter) filters. MATERIALS AND METHODS: Whole blood collected from six volunteers at 4 degrees C or 37 degrees C ...
Krailadsiri P - - 2001
BACKGROUND: The levels of residual red blood cells (RBC) and platelets (PLT) in WBC-reduced plasma are often below the lower detection limit of automated blood cell counters. This study established a novel flow cytometric method for the enumeration of residual RBC and PLT in plasma. Furthermore, their levels in WBC-reduced ...
Sutton M Y - - 2001
A molecular-based subtyping system for Treponema pallidum was used during an investigation of increasing syphilis in Maricopa County, Arizona. Genital ulcer or whole blood specimens from patients with syphilis were assayed by a polymerase chain reaction (PCR) amplification of a T. pallidum DNA polymerase I gene. Positive specimens were typed ...
Wiwanitkit V - - 2001
This study was set in the Division of Laboratory Medicine, Chulalongkorn Hospital. All 2,000 blood specimens were randomly collected using evacuated blood collection by plain or gel vacuum tubes. After collection, each specimen was considered and judged using criteria of specimen rejection to determine how proper the specimen presentations were. ...
Nielsen H J - - 2001
BACKGROUND AND OBJECTIVES: Poor prognosis after resection of primary colorectal cancer may be related to the combination of perioperative blood transfusion and subsequent development of infectious complications. White blood cell--and platelet-derived cancer growth substances, including vascular endothelial growth factor (VEGF), may be involved in this process. Therefore, we studied the ...
Wilcken B - - 2001
The carnitine transporter defect is a potentially fatal but treatable disorder. We used electrospray tandem mass spectrometry in the New South Wales (Australia) Newborn Screening Programme to measure free carnitine and acylcarnitine species in the newborn population. Free carnitine levels in dried blood samples from 149,000 neonates did not vary ...
Schumacher J - - 2001
The evolution of turbulent spots in a parallel shear flow is studied by means of full three-dimensional numerical simulations. The flow is bounded by free surfaces and driven by a volume force. Three regions in the spanwise spot cross section can be identified: a turbulent interior, an interface layer with ...
Watson D G - - 2001
Monitoring of drugs (such as theophylline) with a narrow therapeutic window could be simplified if patients were able to submit blood spots for analysis. This could reduce clinic attendance for venous blood sampling and save staff time. A rapid sensitive method utilizing liquid chromatography-mass spectrometry has been developed to determine ...
Moon M H - - 2001
An upstream ultrafiltration (UU) method is employed for the on-line concentration of collected particle fraction and for the convenient regulation of flow rates in split-flow thin (SPLITT) fractionation. Concentration of the collected particle fraction is necessary to fractionate particles by smaller cutoff diameters in SPLITT fractionation. By introducing a simple ...
Jones J S - - 2001
The measurement of insulin-like growth factor I (IGF-I) from extracted whole dried blood spots on filter paper is a simple method allowing sample collection to be carried out by the patient at home. One drop of blood is applied to a filter card, air dried and posted to the laboratory. ...
Simpson D M - - 2001
Autoregulation maintains cerebral blood flow (CBF) almost constant in the face of changes in arterial blood pressure (ABP). Tests for impairment of this process using only spontaneous fluctuations in ABP, without provoking large variations, are of great clinical interest, and a range of different approaches have previously been applied. Extending ...
Soli M - - 2001
BACKGROUND AND OBJECTIVES: A multicentre trial was set up to evaluate the performance of a new leucodepletion protocol. MATERIALS AND METHODS: Filtration at high haematocrit was started during collection of red blood cell (RBC) products by apheresis with Trima. SAG-M was added after filtration through the filter. Haematocrits and haemoglobin ...
Jamal R - - 2000
The advent of leukocyte filters has enabled effective removal of leukocytes from certain blood products thus avoiding many adverse effects of blood transfusion. Many different materials have been incorporated into these filters to achieve >95% leukocyte removal. In this study we evaluated the efficacy of leukocyte removal of two different ...
Carroll F T - - 2000
Medical examiners frequently rely on the finding of free morphine present in postmortem specimens to assist in certifying deaths associated with narcotics. In vitro hydrolysis of morphine-3-D glucuronide (M3DG) to free morphine was studied using variable specimen pH, initial degree of specimen putrefaction, storage temperature and time, and the effectiveness ...
David-Neto E - - 2000
Evaluation of Cyclosporin A (CyA) blood concentration is imperative in solid organ transplantation in order to achieve maximal immunosuppression with the least side effects. We compared the results of whole blood concentrations of CyA in 50 blood samples simultaneously evaluated by the fluorescent polarization immune assay (TDx) and the enzymatic ...
Chitambar S D - - 2000
BACKGROUND: Venous blood collection is a cumbersome and uncomfortable procedure during hepatitis A surveillance. Collection of capillary blood by finger prick is an alternative method. AIM: To evaluate the reactivity of capillary blood/anti-hepatitis A virus (HAV) IgG stored on filter paper disks for detection of anti-HAV antibody. METHODS: Venous blood ...
Rivero A - - 2000
Phenylketonuria is an inherited metabolic disorder caused by a defect in the hydroxylation of phenylalanine. Newborn screening is crucial for the diagnosis and treatment of this disease. A phenylalanine dehydrogenase-coupled enzymatic assay (Quantase) in microtiter plates for the screening of phenylketonuria was evaluated and compared with our routine method based ...
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