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Results 351 - 400 of 884
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Zurfl?h Marcel R - - 2005
Tetrahydrobiopterin (BH4) deficiency among newborns with hyperphenylalaninemia must be rapidly diagnosed and distinguished from classical phenylketonuria (PKU) to initiate immediately specific treatment and to prevent irreversible neurological damage. The characteristic pattern of urinary pterins makes it possible to differentiate between PKU and BH4 deficiencies, and to identify different variants of ...
- - 2005
Phlebotomists at the Jackson-Madison County General Hospital perform more than 18,000 venipunctures a month to collect blood specimens for laboratory tests. Because each of the blood draws involves handling five or six labels for the tubes that the blood is collected in, it means the hospital prints about 90,000 labels ...
Dammann Christiane E L CE Department of Pediatric Pulmonology and Neonatology, Hannover Medical School, Hannover, Germany. - - 2006
The rapid and reliable identification of biomarkers in the smallest possible amount of blood remains a challenge in biomarker epidemiological research involving preterm newborns. We wanted to explore whether the proteomics approach of 'surface-enhanced laser desorption/ionization-time of flight mass spectrometry' (SELDI-TOF MS) is possible and feasible in whole cord blood ...
Prezeau Nicolas - - 2006
Standardization efforts of real time quantitative PCR (RQ-PCR), such as the Europe Against Cancer (EAC) protocol, are actually essential. However, all the stages of the preanalytical phase (blood collection, conservation and procedures of cellular separation) which influence the ex vivo expression of many genes are not controlled. Various kits for ...
Carducci Claudia - - 2006
BACKGROUND: Guanidinoacetate (GAA) and creatine (Cr) are reliable biochemical markers of primary creatine disorders. The aim of this study was to develop and validate a method for the determination of GAA and Cr in dried blood spot through the use of stable isotope dilution and flow injection analysis (FIA)-ESI-MS/MS. METHODS: ...
Uttayamakul Sumonmal - - 2005
The usage of dried blood spots as specimens for diagnosis and monitoring of HIV-1 infection in Thailand was evaluated. EDTA blood samples, which were collected from 100 HIV seronegative and 109 HIV seropositive individuals, were tested on dried blood spots; Whatman, Schleicher and Schuell (S&S) No. 903 and S&S IsoCode ...
Ikejima Hideaki - - 2005
Current studies indicate that a significant percentage of healthy blood donors carry Chlamydia pneumoniae in their blood. Although the clinical significance of such findings is unknown, eradication of such bacteria from blood components may contribute to transfusion safety. Deletion of C. pneumoniae in Red Blood Cell (RBC) units was accomplished ...
von Lode Piia - - 2006
INTRODUCTION: Normal concentrations of D-Dimer can be used to exclude venous thromboembolism (VTE). However, methods for sensitive and quantitative D-Dimer measurements at the point-of-care (POC) are still limited. MATERIALS AND METHODS: We developed a 10-min, non-competitive immunofluorometric assay for D-Dimer in citrated whole blood and plasma using pre-dispensed reagents dried ...
Unger Juliane K - - 2005
BACKGROUND: Apart from their standard applications, haemofiltration (HF) and plasmafiltration (PF) may provide helpful therapy for sepsis, multiple organ- and acute liver-failure. Some colloids cause either decreases or increases in blood cell agglomeration. We hypothesized that solutions which reduce cell aggregability may lead to both improved filter clearance and better ...
Takahashi Hirokazu - - 2005
This paper provides a detailed process flow for fabricating an easy-to-prepare, inexpensive, dense array of tungsten microelectrodes. We designed the process flow to minimize routine tasks by separating an initial preparation of a master mold from a routine preparation of substrate replication, array assembly and tip processing. Sandblast processing first ...
Judge Barbara B The U.K. Newborn Screening Programme - - 2005
The U.K. Newborn Screening Programme Centre (UKNSPC) launched the first UK-wide standards and policies for newborn blood spot screening, together with a national pre-screening information leaflet for parents in December 2004. These standards are included in a document pack that has been sent recently to all heads of midwifery and ...
Song Eun Young - - 2005
The concentration of Vasoactive Intestinal Peptide (VIP) as measured by recycling immunoaffinity chromatography (RIC) has been reported to be elevated in the blood of patients with autism as compared with normal subjects. In this study, we have developed a "Competitive Fluorescent Microsphere Immunoassay" (cFMI) in which VIP competes with biotinylated ...
Wells Alan - - 2005
The effects of arginine vasotocin on the pattern of glomerular perfusion in an elasmobranch fish were examined using an in situ perfused renal trunk preparation. A significant antidiuresis was coupled with a marked reduction in the proportion of filtering glomeruli (86%-25%) and an increase in the proportion of perfused but ...
Haltern Claudia - - 2005
During extracorporeal circulation, activation of the complement system as a bioincompatibility reaction upon contact with artificial surfaces is well known. This may induce adverse effects for the patient as well as for the bioreactor when using a hybrid liver support system. At present, the achievable clearance rates in hybrid liver ...
Maucher Jennifer M - - 2005
Domoic acid (DA), an analog of the excitatory amino acid glutamate, is produced by the diatom genus Pseudo-nitzschia and acts as a neurotoxin in humans. During diatom blooms, DA can contaminate shellfish, as well as other filter feeding organisms, and can be transferred by ingestion to higher trophic levels, including ...
Hein Leanne K - - 2005
BACKGROUND: Mucopolysaccharidosis type VI (MPS VI) is a lysosomal storage disorder (LSD), which is caused by a deficiency in the enzyme N-acetylgalactosamine 4-sulfatase (4-sulfatase). MPS VI is characterized by severe skeletal abnormalities, somatic tissue pathology and early death. Treatment possibilities include bone marrow transplantation (BMT) and enzyme replacement therapy (ERT; ...
Wildberger Joachim Ernst - - 2005
PURPOSE: To evaluate the size and quantity of downstream emboli after thrombectomy using the Arrow-Trerotola Percutaneous Thrombolytic Device (PTD) with or without temporary filtration for extensive iliofemoral and iliocaval thrombi in an in vitro flow model. METHODS: Iliocaval thrombi were simulated by clotted bovine blood in a flow model (semilucent ...
Kløve Bjørn - - 2005
Controlled experiments were carried out in a mesoscale subsurface flow sand filter treating municipal wastewater from a single household. The system consisted of a 50 cm high vertical flow column (pre-filter) with unsaturated flow and a 3 m long horizontal subsurface flow unit (main filter) with saturated flow. Fluxes of ...
Hartmann Jens - - 2005
AIM: The newly developed 'Microspheres based Detoxification System' (MDS) designed for any extracorporeal adsorption therapy uses microparticles as adsorbents characterized by a size of 1-20 microm in diameter which are recirculated in the secondary (filtrate) circuit connected to a hollow fiber filter located in the primary (blood) circuit. In the ...
Langenbeck U - - 2005
The need for regular blood-drawing in the management of chronic metabolic disorders may negatively influence the compliance of patients and their parents; noninvasive analytical procedures could well alleviate this burden. Using data obtained in six adult probands with phenylketonuria, we evaluate the feasibility of noninvasive prediction of phenylalanine blood concentrations ...
Brandl M - - 2005
BACKGROUND: A microsphere-based detoxification system is an adsorption system, whereby microadsorbent particles having diameters of 1-20 microm circulate in an extracorporeal filtrate circle. A thin-wall hollow-fiber membrane filter separates the microparticle-plasma suspension from the bloodstream. For patient safety, it is necessary to have a means to detect membrane ruptures that ...
Li Gang G Coll. of Precision Instrum. & Opto-electron. Eng., Tianjin - - 2005
We developed a brand-new method that fits the non-invasive blood component concentration measurement: the dynamic spectrum (DS) method. With use of this method, it was possible to eliminate the main interference of the individual discrepancy and extract some kinds of the blood component concentration by the analytic method. The definition, ...
Golabbakhsh M M Department of Electrical and Computer Engineering, University of Manitoba Winnipeg, Manitoba, - - 2005
In this study, average power of tracheal sound (Pave) was used to estimate flow by parametric method as well as adaptive filters as a nonparametric method. Based on some preliminary studies, an exponential model was used for describing the relationship between flow and Pave for parametric method. It was assumed ...
Zhang S S State Key Laboratory of Bioelectronics, Southeast University Nanjing, China, - - 2005
This paper describes a new method to fabricate large scale 3D weir-type filter for cell fractionation. The 3D structure is realized by a conformal bonding of two separate PDMS (poly-dimethyloxane) layers with in-situ microchannels which are formed by the conventional soft lithographic techniques. Moreover, the microfluidics channels on the two ...
Morrison Matthew A - - 2004
Artifacts associated with the fractionation of colloids in a freshwater sample were investigated for conventional membrane filtration (0.45 microm cutoff), and two tangential flow ultrafiltration cartridges (0.1 microm cutoff and 3000 MW cutoff). Membrane clogging during conventional filtration removed some colloids smaller than 0.1 microm in diameter, much smaller than ...
Yadav Jay S - - 2004
Emboli prevention devices have become an integral part of carotid stenting and have dramatically lowered the risk of stroke during the procedure. A variety of devices are available with different performance characteristics. Filter devices are the current mainstay and have high technical success rates. Slow flow through the filters can ...
Yamashita Akihiro C - - 2004
BACKGROUND: Many synthetic polymeric membranes have been widely used for blood purification therapies for removing larger solutes and for better blood compatibility. METHODS: Five different filters with polyester polymer alloy (PEPA) membrane (Nikkiso) that included polyvinylpyrrolidone (PVP), that is, standard-pore-size FLX (PEPA with PVP(-)), standard-pore-size FDX (PEPA with PVP(+)), new ...
Baldwin Ian - - 2004
OBJECTIVE: Reductions in blood flow rate may occur undetected during peristaltic pumping of blood through continuous renal replacement therapy circuits. We investigated whether undetected reductions in blood flow rate occur during continuous veno-venous haemofiltration, and whether they are correlated with filter life. PATIENTS: Twelve patients receiving continuous veno-venous haemofiltration in ...
Stroncek David F - - 2004
BACKGROUND Red blood cell (RBC) components from donors with sickle cell trait (Hb AS) often occlude white blood cell (WBC) reduction filters. Techniques were investigated to successfully filter Hb AS donor blood by increasing the Hb oxygen saturation with storage bags and conditions suitable for transfusion products. STUDY DESIGN AND ...
Yamato Osamu - - 2004
This report describes a rapid and simple method for mutation screening of G(M1) gangliosidosis in Shiba dogs by direct amplification of DNA from canine whole-blood specimens using a novel polymerase chain reaction (PCR) reagent cocktail, which can eliminate the DNA extraction process and amplify the genomic DNA directly from human ...
Paunovic Dragica - - 2004
BACKGROUND: Whole-blood (WB) leukoreduction filters in current use retain the majority of PLTs. A new whole-blood filter, which retains significantly fewer of the PLTs (or saves PLTs [WB-SP]), has been developed. The performance characteristics of the WB-SP filter have been evaluated in a multicenter study. STUDY DESIGN AND METHODS: A ...
Heiden M - - 2004
The study evaluated the quality of plasma obtained after whole-blood filtration with four different polyester filters and one polyurethane filter. The activities of coagulation factors and proteinase inhibitors were not or only negligibly affected by filtration, in all experiments. Filtration did not increase markers of clotting and fibrinolysis. Only a ...
Rentas F J - - 2004
BACKGROUND: In late January 2003, some blood centers and hospitals throughout the US voluntarily sus-pended the use of some RBC and plasma units for trans-fusion due to the presence of unknown white particulate matter (WPM) in these units. To better understand the WPM phenomena, a number of technologies were used ...
Simpson David M - - 2004
In order to assess blood flow control, the relationship between blood pressure and blood flow can be modeled by linear filters. We present a bootstrap method, which allows the statistical analysis of an index of blood flow control that is obtained from constrained system identification using an established set of ...
Karthik V Vishnu - - 2004
Filter holders and continuous air monitors are used extensively in the nuclear industry. It is important to minimize leakage in these devices, and, in recognition of this consideration, a limit on leakage for sampling systems is specified in; however, the protocol given in the standard is really germane to measurement ...
De Godoy José Maria Pereira - - 2004
Inferior vena cava filters are indicated for the prevention of pulmonary embolism when anticoagulation using heparin has failed or is contraindicated. The aim of this study was to assess in an in-vitro setting the efficiency of a new inferior vena cava filter to intercept emboli. The new filter, stent-filter, was ...
Gatti A M - - 2004
The research deals with new scanning electron microscopic evaluations of the interface between blood and explanted temporary vena cava filters from patients affected by blood disorders. The biological tissues adherent to the filter and the small thrombi formed in vivo were detached from the metallic structure of the device, fixed, ...
Beard Martin J - - 2004
BACKGROUND: Sickle cell trait donations can block leukodepletion (LD) filters or fail to LD, but the variables affecting blockage are unclear. STUDY DESIGN AND METHODS: To identify critical variables for further study, the relationship was investigated between filter blockage and donor characteristics, processing conditions, PLT and coagulation system activation, and ...
Wilkes A R - - 2004
When tested according to the European standard, the performance of breathing system filters is determined at a flow of 15 or 30 l.min-1 for filters intended for use with paediatric or adult patients, respectively. However, higher flows of gas may pass through a filter in some circumstances. The penetration of ...
Pérez-Pujol Sílvia - - 2004
BACKGROUND: Filtered PLT concentrates (PCs) were prepared in plasma pooling three (for children) or six buffy coats (BCs; for adults) after holding them a maximum of 4 hours (blood bags collected in the afternoon) or 18 hours (blood bags collected in the morning). STUDY DESIGN AND METHODS: With flow cytometry, ...
Pasqualetti Daniela - - 2004
Over the last few years, quality system requirements have been introduced for blood components. The necessary compliance with standard productions will have a considerable impact on Blood Banks. The introduction of automated methods is the most satisfactory means to meet these requirements for blood component preparation. A new device has ...
Schölvinck Elisabeth - - 2004
The gamma interferon secretion levels of 52 adults whose T cells had been stimulated with the Mycobacterium tuberculosis antigens CFP-10 and ESAT-6 were measured by enzyme-linked immunospot methods and a whole-blood-based assay. The test results correlated well (r = 0.689, P < 0.0001). Its simple format and use of only ...
Césaire Raymond - - 2004
BACKGROUND: WBC depletion by filtration may prevent the transmission of HTLV-I, which requires cell-to-cell contact. The removal of HTLV-I-infected cells in routinely filtered blood cell components was measured. STUDY DESIGN AND METHODS: The study was conducted in Martinique where systematic screening for HTLV-I and -II and universal leukoreduction are mandatory. ...
Johnson D W - - 2004
Malonylcarnitine and glutarylcarnitine are important diagnostic metabolites in the screening of dried blood spots by tandem mass spectrometry. The stability of these compounds in spiked blood spots stored at room temperature was studied. Both showed biphasic curves. The malonylcarnitine concentration dropped to 61% in 38 days and averaged 51% +/- ...
Yang Hong - - 2004
The technology core of biological removal of iron and manganese by contact oxidation is iron and manganese oxidizing bacteria. This article made a study on down flow and up flow filtration, the results show that down flow filtration of the homogenous filter medium is more adapted to the reproduction of ...
Mwaba Peter - - 2003
As antiretroviral drugs become widely available in developing countries, practical, field-friendly, and cheap methods of measuring CD4+ lymphocyte counts need to be developed. We tested use of whole blood spots dried on filter paper to measure CD4+ lymphocyte counts. We obtained blood from 42 HIV-1-infected patients from Zambia. We dried ...
Spiehler Vina - - 2003
The object of this study was to evaluate the suitability of the Neogen Corporation microtiter plate enzyme-linked immunoassay (ELISA) for cocaine and metabolites for screening of postmortem blood. Sixty-five postmortem whole blood specimens were obtained from drug-involved deaths that had been screened and confirmed positive for cocaine and/or benzoylecgonine (BE). ...
Gallippi Caterina M - - 2003
Blind source separation (BSS) for adaptive filtering is presented in application to imaging both physiological and acoustic radiation force impulse (ARFI)-induced tissue and blood motion in the common carotid artery. The collected raw radiofrequency (RF) data includes vessel wall motion, blood flow and ARFI-induced motion. In the context of these ...
Visconti Micaela Rios - - 2004
To assess removal of cytomegalovirus (CMV) by leukocyte depletion (LD) filters, we developed a spiking model of latent virus using peripheral blood mononuclear cells (PBMCs) infected by coculture with CMV-infected human fibroblasts. Infected PBMCs were purified by dual magnetic column selection and then spiked into whole blood units or buffy ...
Y. Usui,K. Nakano,Y. Motonaga
The replacement of human visual inspection for eggs with blood spots using transmitted light currently employed at grading and packing centers with a method using near infrared spectroscopy (NIRS) was studied. The determination is made using wavelength absorbance data since the hemoglobin in blood mixed in eggs with blood spots ...
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