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Results 351 - 400 of 911
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Huang Huan H Medical School, Nanjing University, - - 2006
To demonstrate an inexpensive method for typing gastric cancer related single nucleotide polymorphisms (SNPs) using whole blood or paper-dried blood as starting materials. PCR amplification is directly carried out from the whole blood or paper-dried blood sample without any DNA extraction step. Before PCR, a blood sample, four primers, and ...
Faqih AbdulMobeen - - 2006
This paper describes a new method to quantitatively measure the flow characteristics of unconfined cohesive powders in a rotating drum. Cohesion plays an important role, affecting flow properties/characteristics, mixing rates, and segregation tendencies. The method relies on measuring the change in center of mass of the powder bed as it ...
Holub Margareta - - 2006
BACKGROUND: Detection of amino acids (AA), acylcarnitines (AC), and guanidinoacetate (GAA) in dried blood spots by tandem mass spectrometry has made it possible to detect different inborn errors of metabolism in neonatal screening programs. Despite its proven sensitivity many issues related to sample preparation remain unsolved. Hematocrit has a profound ...
Suliman F - - 2006
Effects of the inlet and outlet position on flow patterns of saturated fluids in a horizontal subsurface-flow constructed wetland were investigated experimentally using a quasi two-dimensional flow cell representing a vertical cross-section in the longitudinal direction of the wetland. The filter medium consisted of glass beads that were either uniformly ...
Robbe-Austerman S - - 2006
Our objective was to evaluate the effects of time and temperature on whole blood used in the gamma interferon enzyme-linked immunosorbent assay (IFN-gamma ELISA) for paratuberculosis along with evaluating four potential positive controls, and four different mycobacterial antigens for the ELISA. Nine adult Holstein cattle naturally infected with Mycobacterium avium ...
Cardo Lisa J - - 2006
BACKGROUND: Leishmania is an intracellular parasite of monocytes transmissible by transfusion. The feasibility of reducing Leishmania with leukodepletion filters was studied. At collection, infected blood contains the amastigote form of Leishmania within monocytes. Amastigotes cause the rupture of monocytes releasing free amastigotes that convert to promastigotes, which exist extracellularly at ...
Sowemimo-Coker S O - - 2006
BACKGROUND AND OBJECTIVES: Three recent probable cases of transmission of a variant of human Creutzfeldt-Jakob disease (vCJD) through blood transfusion suggest that the disease can be transmitted through transfusion of blood components from presymptomatic blood donors. In this study, we investigated the performance of a new filter for reducing the ...
Gatti A M - - 2006
Vena cava filters are the most commonly used mechanical devices to prevent pulmonary embolism. A retrievable permanent filter has been available since 1999. That has allowed the direct study of thrombi captured in humans and the punctual interaction of blood and device at long and short term. Through traditional histologic ...
Zucker B-A - - 2006
Inhalation of bioaerosols from animal houses can induce acute inflammatory reactions in the respiratory tract. Determination of the concentration of airborne endotoxins is widely used to characterize this risk. In this study, the activity of bioaerosol samples from a duck-fattening unit to induce interleukin-1beta (IL-1beta) in human blood and to ...
Kroll Charles A - - 2006
Wilson disease is an autosomal recessive disorder of copper transport, caused by the reduced or absent function of the Wilson disease gene ATP7B on chromosome 13. The disease is characterized by reduced incorporation of copper into the ceruloplasmin protein and reduced excretion of copper into the bile. Wilson disease is ...
Kim Dong Sung - - 2006
Blood typing is the most important test for both transfusion recipients and blood donors. In this paper, a low cost disposable blood typing integrated microfluidic biochip has been designed, fabricated and characterized. In the biochip, flow splitting microchannels, chaotic micromixers, reaction microchambers and detection microfilters are fully integrated. The loaded ...
Arai Meiji - - 2006
Deficiency of glucose-6-phosphate dehydrogenase (G6PD) causes acute hemolytic anemia triggered by oxidative drugs such as primaquine. It is therefore essential in malaria-endemic areas for malaria patients to be confirmed for their G6PD activity before taking primaquine. The WST-8 method, a newly established screening method for G6PD deficiency, has been demonstrated ...
Gelb Michael H MH Department of Chemistry, University of Washington, Seattle, Washington, USA. - - 2006
Tandem mass spectrometry is currently used in newborn screening programmes to quantify the level of amino acids and acylcarnitines in dried blood spots for detection of metabolites associated with treatable diseases. We have developed assays for lysosomal enzymes in rehydrated dried blood spots in which a set of substrates is ...
Thorslund Sara - - 2006
Miniaturized biochemical devices in glass, silicon and polymer materials are starting to find their way from the academic laboratories to real-life applications. However, most attention has been given to miniaturize the downstream functions of various microfluidic systems, leaving the sample introduction and preparation steps to more conventional, bulkier solutions. For ...
Dean Caroline J - - 2006
BACKGROUND: Mucopolysaccharidosis type II (MPS II) is a lysosomal storage disorder related to a deficiency in the enzyme iduronate-2-sulfatase (IDS). Clinical trials of enzyme replacement therapy are in progress, but effective treatment will require screening assays to enable early detection and diagnosis of MPS II. Our study evaluated the diagnostic ...
Song Eun Young - - 2006
Calcitonin gene-related peptide (CGRP) has roles as a neurotransmitter, neuromodulator and trophic factor. CGRP has been reported to be elevated in neonatal blood of children with autism or mental retardation as compared with normal subjects by recycling immuno-affinity chromatography (RIC). While CGRP detection in neonatal blood is thus important, it ...
Kim Hyun-Joo - - 2006
Hollow-fiber flow field-flow fractionation (HF FlFFF) was applied for the separation and size characterization of airborne particles which were collected in a municipal area and prefractionated into four different-diameter intervals >5.0, 2.5-5.0, 1.5-2.5, <1.5 microm) by continuous split-flow thin (SPLIIT) fractionation. Experiments demonstrated the possibility of utilizing a hollow-fiber module ...
Unger J K - - 2006
Flow distributions are critical determinants in the function of hemofilters. Despite their importance, however, flow distributions cannot currently be measured in filters during experimental or clinical applications. Here, we demonstrate that the thermal conduction properties of extracorporeal circuits may provide a tool to overcome this limitation. More specifically, we show ...
Supali Taniawati - - 2006
The prevalence of lymphatic filariasis was estimated by PCR-based pool screening of night blood collected from 865 individuals living in ten areas endemic for Wuchereria bancrofti, Brugia malayi or B. timori in Indonesia. A total of 232 microfilaraemics were identified by filtration of 1 ml of blood. The microfilaria (mf) ...
Tsai Y H - - 2006
Six fishermen were victims (including one death) of food poisoning from unknown fish on their boat in central Taiwan Strait, in April 2001. The symptoms were like those of tetrodotoxin (TTX) poisoning. As there was no remaining fish, a new protocol was developed to determine TTX in the urine and ...
Molle P - - 2006
Vertical flow constructed wetlands (VFCWs) have been very successful in France over the last 5 years. The sizing of VFCWs is still roughly based on organic load acceptance with slight clear water intrusion into the sewerage system which is often wrong in the context of small communities. To specify the ...
Holdich Richard - - 2006
In filtration, the concept of pore size is not easy to define. In microfiltration, there are numerous advantages in employing a surface filtering membrane, rather than one relying on depth filtration mechanisms from a tortuous pore flow channel. Modern manufacturing techniques provide means to produce surface filtering membranes. For filtration, ...
Li Lixia - - 2006
OBJECTIVES: In response to increasing numbers of states in the US that test newborn babies for cystic fibrosis (CF), the Newborn Screening Quality Assurance Programme initiated a pilot proficiency testing programme for immunoreactive trypsinogen (IRT), the biomarker for CF. Dried blood spot specimens (DBS) were used to evaluate the performance ...
Osorio Francisco - - 2006
The primary objective of this research study was to test the applicability and optimize the design parameters of a system of biological aerated filters in order to obtain an optimal effluent from the citrus industry, which would allow its drainage into the municipal sewer system. Expanded clay was used as ...
Dubay Shelli A - - 2006
We investigated the feasibility of using whole blood dried on paper strips as a means to collect antibody prevalence data for the epizootic hemorrhagic disease viruses (EHDV) and bluetongue viruses (BTV) from hunter-harvested male mule deer (Odocoileus hemionus) in October 2002 from Arizona, USA. We compared antibody prevalence estimates in ...
Quraishi Rizwana - - 2006
Adaptation of assays on dried blood has advantages of ease of collection, transportation, minimal invasiveness and requirement of small volume. A method for extraction and estimation of triglyceride from blood spots dried on filter paper (Whatman no. 3) has been developed. A single dried blood spot containing 10 muL blood ...
Zurfl?h Marcel R - - 2005
Tetrahydrobiopterin (BH4) deficiency among newborns with hyperphenylalaninemia must be rapidly diagnosed and distinguished from classical phenylketonuria (PKU) to initiate immediately specific treatment and to prevent irreversible neurological damage. The characteristic pattern of urinary pterins makes it possible to differentiate between PKU and BH4 deficiencies, and to identify different variants of ...
- - 2005
Phlebotomists at the Jackson-Madison County General Hospital perform more than 18,000 venipunctures a month to collect blood specimens for laboratory tests. Because each of the blood draws involves handling five or six labels for the tubes that the blood is collected in, it means the hospital prints about 90,000 labels ...
Dammann Christiane E L CE Department of Pediatric Pulmonology and Neonatology, Hannover Medical School, Hannover, Germany. - - 2006
The rapid and reliable identification of biomarkers in the smallest possible amount of blood remains a challenge in biomarker epidemiological research involving preterm newborns. We wanted to explore whether the proteomics approach of 'surface-enhanced laser desorption/ionization-time of flight mass spectrometry' (SELDI-TOF MS) is possible and feasible in whole cord blood ...
Prezeau Nicolas - - 2006
Standardization efforts of real time quantitative PCR (RQ-PCR), such as the Europe Against Cancer (EAC) protocol, are actually essential. However, all the stages of the preanalytical phase (blood collection, conservation and procedures of cellular separation) which influence the ex vivo expression of many genes are not controlled. Various kits for ...
Carducci Claudia - - 2006
BACKGROUND: Guanidinoacetate (GAA) and creatine (Cr) are reliable biochemical markers of primary creatine disorders. The aim of this study was to develop and validate a method for the determination of GAA and Cr in dried blood spot through the use of stable isotope dilution and flow injection analysis (FIA)-ESI-MS/MS. METHODS: ...
Uttayamakul Sumonmal - - 2005
The usage of dried blood spots as specimens for diagnosis and monitoring of HIV-1 infection in Thailand was evaluated. EDTA blood samples, which were collected from 100 HIV seronegative and 109 HIV seropositive individuals, were tested on dried blood spots; Whatman, Schleicher and Schuell (S&S) No. 903 and S&S IsoCode ...
Ikejima Hideaki - - 2005
Current studies indicate that a significant percentage of healthy blood donors carry Chlamydia pneumoniae in their blood. Although the clinical significance of such findings is unknown, eradication of such bacteria from blood components may contribute to transfusion safety. Deletion of C. pneumoniae in Red Blood Cell (RBC) units was accomplished ...
von Lode Piia - - 2006
INTRODUCTION: Normal concentrations of D-Dimer can be used to exclude venous thromboembolism (VTE). However, methods for sensitive and quantitative D-Dimer measurements at the point-of-care (POC) are still limited. MATERIALS AND METHODS: We developed a 10-min, non-competitive immunofluorometric assay for D-Dimer in citrated whole blood and plasma using pre-dispensed reagents dried ...
Unger Juliane K - - 2005
BACKGROUND: Apart from their standard applications, haemofiltration (HF) and plasmafiltration (PF) may provide helpful therapy for sepsis, multiple organ- and acute liver-failure. Some colloids cause either decreases or increases in blood cell agglomeration. We hypothesized that solutions which reduce cell aggregability may lead to both improved filter clearance and better ...
Takahashi Hirokazu - - 2005
This paper provides a detailed process flow for fabricating an easy-to-prepare, inexpensive, dense array of tungsten microelectrodes. We designed the process flow to minimize routine tasks by separating an initial preparation of a master mold from a routine preparation of substrate replication, array assembly and tip processing. Sandblast processing first ...
Judge Barbara B The U.K. Newborn Screening Programme - - 2005
The U.K. Newborn Screening Programme Centre (UKNSPC) launched the first UK-wide standards and policies for newborn blood spot screening, together with a national pre-screening information leaflet for parents in December 2004. These standards are included in a document pack that has been sent recently to all heads of midwifery and ...
Song Eun Young - - 2005
The concentration of Vasoactive Intestinal Peptide (VIP) as measured by recycling immunoaffinity chromatography (RIC) has been reported to be elevated in the blood of patients with autism as compared with normal subjects. In this study, we have developed a "Competitive Fluorescent Microsphere Immunoassay" (cFMI) in which VIP competes with biotinylated ...
Wells Alan - - 2005
The effects of arginine vasotocin on the pattern of glomerular perfusion in an elasmobranch fish were examined using an in situ perfused renal trunk preparation. A significant antidiuresis was coupled with a marked reduction in the proportion of filtering glomeruli (86%-25%) and an increase in the proportion of perfused but ...
Haltern Claudia - - 2005
During extracorporeal circulation, activation of the complement system as a bioincompatibility reaction upon contact with artificial surfaces is well known. This may induce adverse effects for the patient as well as for the bioreactor when using a hybrid liver support system. At present, the achievable clearance rates in hybrid liver ...
Maucher Jennifer M - - 2005
Domoic acid (DA), an analog of the excitatory amino acid glutamate, is produced by the diatom genus Pseudo-nitzschia and acts as a neurotoxin in humans. During diatom blooms, DA can contaminate shellfish, as well as other filter feeding organisms, and can be transferred by ingestion to higher trophic levels, including ...
Hein Leanne K - - 2005
BACKGROUND: Mucopolysaccharidosis type VI (MPS VI) is a lysosomal storage disorder (LSD), which is caused by a deficiency in the enzyme N-acetylgalactosamine 4-sulfatase (4-sulfatase). MPS VI is characterized by severe skeletal abnormalities, somatic tissue pathology and early death. Treatment possibilities include bone marrow transplantation (BMT) and enzyme replacement therapy (ERT; ...
Wildberger Joachim Ernst - - 2005
PURPOSE: To evaluate the size and quantity of downstream emboli after thrombectomy using the Arrow-Trerotola Percutaneous Thrombolytic Device (PTD) with or without temporary filtration for extensive iliofemoral and iliocaval thrombi in an in vitro flow model. METHODS: Iliocaval thrombi were simulated by clotted bovine blood in a flow model (semilucent ...
Kløve Bjørn - - 2005
Controlled experiments were carried out in a mesoscale subsurface flow sand filter treating municipal wastewater from a single household. The system consisted of a 50 cm high vertical flow column (pre-filter) with unsaturated flow and a 3 m long horizontal subsurface flow unit (main filter) with saturated flow. Fluxes of ...
Hartmann Jens - - 2005
AIM: The newly developed 'Microspheres based Detoxification System' (MDS) designed for any extracorporeal adsorption therapy uses microparticles as adsorbents characterized by a size of 1-20 microm in diameter which are recirculated in the secondary (filtrate) circuit connected to a hollow fiber filter located in the primary (blood) circuit. In the ...
Langenbeck U - - 2005
The need for regular blood-drawing in the management of chronic metabolic disorders may negatively influence the compliance of patients and their parents; noninvasive analytical procedures could well alleviate this burden. Using data obtained in six adult probands with phenylketonuria, we evaluate the feasibility of noninvasive prediction of phenylalanine blood concentrations ...
Brandl M - - 2005
BACKGROUND: A microsphere-based detoxification system is an adsorption system, whereby microadsorbent particles having diameters of 1-20 microm circulate in an extracorporeal filtrate circle. A thin-wall hollow-fiber membrane filter separates the microparticle-plasma suspension from the bloodstream. For patient safety, it is necessary to have a means to detect membrane ruptures that ...
Li Gang G Coll. of Precision Instrum. & Opto-electron. Eng., Tianjin - - 2005
We developed a brand-new method that fits the non-invasive blood component concentration measurement: the dynamic spectrum (DS) method. With use of this method, it was possible to eliminate the main interference of the individual discrepancy and extract some kinds of the blood component concentration by the analytic method. The definition, ...
Golabbakhsh M M Department of Electrical and Computer Engineering, University of Manitoba Winnipeg, Manitoba, - - 2005
In this study, average power of tracheal sound (Pave) was used to estimate flow by parametric method as well as adaptive filters as a nonparametric method. Based on some preliminary studies, an exponential model was used for describing the relationship between flow and Pave for parametric method. It was assumed ...
Zhang S S State Key Laboratory of Bioelectronics, Southeast University Nanjing, China, - - 2005
This paper describes a new method to fabricate large scale 3D weir-type filter for cell fractionation. The 3D structure is realized by a conformal bonding of two separate PDMS (poly-dimethyloxane) layers with in-situ microchannels which are formed by the conventional soft lithographic techniques. Moreover, the microfluidics channels on the two ...
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