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Results 251 - 300 of 892
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Fokkema M Rebecca - - 2009
BACKGROUND: This study evaluates HbA1c measurements from dried blood spots collected on filter paper and compares HbA1c from filter paper (capillary blood) with HbA1c measured in venous blood. METHODS: Patient satisfaction was evaluated using a questionnaire. The performance with the filter paper method was assessed by comparing HbA1c results from ...
Bhatti Parveen - - 2009
Collection of buccal cells from saliva for DNA extraction offers a less invasive and convenient alternative to venipuncture blood collection that may increase participation in genetic epidemiologic studies. However, dried blood spot collection, which is also a convenient method, offers a means of collecting peripheral blood samples from which analytes ...
Garcia Boy Regine - - 2008
The use of dried blood spots (DBS) which has successfully been introduced in neonatal metabolic screening is an appropriate method to reduce virus infection risk to a minimum, facilitating regular mailing and handling of samples in the laboratory. Injection diacetylmorphine use is notably associated with a prevalence of infection and ...
Ferner Matthew C - - 2008
Suspension feeders rely on filter structures of a variety of forms to capture food particles. Much effort has been devoted to examining the operation of such filters, but mechanistic evaluations have generally represented filter elements with artificially stiff cylinders. We extended this previous work to investigate how bending affects the ...
Matamoros Víctor - - 2009
Occurrence and removal efficiencies of 13 pharmaceuticals and personal care products (PPCPs) as well as BOD(5), TSS and NH(4)(+) were evaluated for the first time in thirteen onsite household secondary wastewater treatment systems, including two compact biofilters followed by Filtralite-P filter units, two biological sand filters, five horizontal subsurface flow ...
Stevens Dean Y - - 2008
As part of an effort to create a point-of-care diagnostic system for the developing world, we present a microfluidic flow-through membrane immunoassay with on-card dry reagent storage. By preserving reagent function, the storage and reconstitution of anhydrous reagents enables the devices to remain viable in challenging, unregulated environmental conditions. The ...
Wong Amy P - - 2008
This paper demonstrates that a hand-powered egg beater can be modified to serve as a centrifuge for separating plasma from human whole blood. Immunoassays used to diagnose infectious diseases often require plasma from whole blood, and obtaining plasma typically requires electrically-powered centrifuges, which are not widely available in resource-limited settings. ...
Haak Peterson T - - 2009
State laws in the USA mandate that blood be drawn from all newborn infants to screen for health-threatening conditions. These screening assays consume only a small portion of the blood samples, which are collected on filter paper ('Guthrie') cards. Many states archive unused blood spots, often in unrefrigerated storage. While ...
Forzán María J - - 2008
The introduction of a new group of dendrobatid frogs to an established captive amphibian collection was followed by several acute mortalities in both resident and introduced frog populations. Chytridiomycosis, caused by Batrachochytrium dendrobatidis, was diagnosed by histology in two of the dead frogs. Following the diagnosis, all amphibians were moved ...
Tan, Jocelyn
Whole blood separation is a crucial step prior to the analysis of a whole blood sample in a lateral flow assay (LFA). While there are numerous diagnostic applications that utilize the technique for rapid and sensitive analysis, few are able to directly work with human whole blood samples and provide ...
Eddings Mark A - - 2008
Limitations in depositing ligands using conventional micro-array pin spotting have hindered the application of surface plasmon resonance imaging (SPRi) technology. To address these challenges we introduce a modification to our continuous-flow micro-spotting technology that improves ligand deposition. Using Flexchip protein A/G and neutravidin capturing surfaces, we demonstrate that our new ...
Haase M - - 2008
PURPOSE: A novel type of adsorptive plasma filtering device (ETX-A) capable of removing endotoxin from blood in a single step has recently been developed using nanotechnology. METHODS: In a miniaturized, ex vivo model of extracorporeal circuits, we tested the capacity to reduce plasma cytokine concentration of ETX-A filters in comparison ...
Smith D P - - 2008
Presence of blood spots in eggs has been correlated with a greater rate of Salmonella Enteritidis contamination. Therefore, this study was conducted to determine whether Salmonella inoculated into egg albumen with naturally occurring blood spots would survive or grow. In each of 3 trials, white shell table eggs with blood ...
Funk William E WE Department of Environmental Sciences and Engineering, School of Public Health, University of California at Chapel Hill, Chapel Hill, NC, - - 2008
Adducts of reactive chemicals with hemoglobin (Hb) or human serum albumin can be used as biomarkers of internal doses of carcinogens. Because dried blood spots are easier to collect and store than conventional venous blood samples, they encourage applications of biomarkers of exposure in large epidemiologic studies. In addition, neonatal ...
Tan Hsih Yin - - 2008
Sarin (C(4)H(10)FO(2)P,O-isopropyl methylphosphonofluoridate) is a colourless, odourless and highly toxic phosphonate that acts as a cholinesterase inhibitor and disrupts neuromuscular transmission. Sarin and related phosphonates are chemical warfare agents, and there is a possibility of their application in a military or terrorist attack. This paper reports a lab-on-a-chip device for ...
Moonen Adrianus F C M - - 2008
A pilot study was undertaken to evaluate whether filters integrated in postoperative retransfusion systems affect the amount of blood cells retransfused after total knee arthroplasty. Twenty-two consecutive patients received either the Donor retransfusion system (n=12 patients) or the Bellovac ABT retransfusion system (n=10). Both systems differ with respect to the ...
Lai Wei H - - 2008
Clenbuterol, which may cause symptoms of increased heart rate, muscular tremors, headache, nausea, and muscular cramps in patients, has been prohibited for consumption in many countries including the European Union, the United States, and China. A rapid lateral-flow strip assay was developed in our laboratory, and results obtained with this ...
Smets Koenraad - - 2008
A fatal case of Coxsackie B3 myocarditis in a neonate is described. The clinical features became evident in the 3rd week of life, but enteroviral RNA was detected in the dried blood spot of the baby collected on day 4 after birth. This is the first report on the detection ...
Yu Alfred C H - - 2008
Because of their adaptability to the slow-time signal contents, eigen-based filters have shown potential in improving the flow detection performance of color flow images. This paper proposes a new eigen-based filter called the Hankel-SVD filter that is intended to process each slowtime ensemble individually. The new filter is derived using ...
Lakshmy Ramakrishnan - - 2008
The collection of dried blood spots on filter paper offers a powerful tool in screening programs and in large population-based surveys. The method has the advantage of being less invasive and relatively painless and is particularly suitable for collection in neonates and the elderly. Blood can be collected and transported ...
Riley Jeffrey B JB Circulation Technology Division, School of Allied Medical Professions, The Ohio State University, Columbus, Ohio, USA. - - 2008
Arterial line filters (ALFs) are arguably the most important component in the cardiopulmonary bypass circuit to protect the patient from gaseous macro- and micro-emboli (GME) originating in the perfusion circuit. The GME separating ability of 10 ALFs was ranked according to seven performance criteria. Ten ALFs rated between 20 and ...
Fachiroh J - - 2008
Dried-blood (DB) samples on filter paper are considered clinical specimens for diagnostic use because of the ease of collection, storage, and transport. We recently developed a synthetic-peptide-based immunoglobulin A (IgA) (EBNA1 plus viral capsid antigen [VCA]-p18) enzyme-linked immunosorbent assay (ELISA) for nasopharyngeal carcinoma (NPC) screening. Here, we evaluate the use ...
Inoue Naoki - - 2008
Although the use of dried blood spots has been proposed for screening of newborns with congenital cytomegalovirus infection, viral loads in blood were significantly smaller than those in urine (P < 0.001), and DNA recovery from dried blood spots using the thermal shock procedure was inefficient. In contrast, our urine-based ...
Kane Coumba Toure - - 2008
Measurement of viral load in plasma remains the best marker for the follow-up of antiretroviral therapy. However, its use is limited in developing countries due to the lack of adequate facilities and equipment, and cryopreservation of plasma during storage and transportation. Practical and reliable methods adapted to field conditions for ...
Ikeda Yuji - - 2008
Although continuous hemodiafiltration (CHDF) has been widely accepted in the management of complicated acute renal failure, the requirement for prolonged continuous systemic anticoagulation appears to be a major drawback. We herein describe the case of a patient who developed postoperative multiple organ failure and received CHDF therapy with partial blood ...
Roffi Marco - - 2008
PURPOSE: To investigate the impact of filter design on blood flow impairment in the internal carotid artery (ICA) among patients undergoing carotid artery stenting (CAS) using filter-type emboli protection devices (EPD). METHODS: Between July 2003 and March 2007, 115 filter-protected CAS procedures were performed at an academic institution in 107 ...
Thibault L - - 2008
BACKGROUND AND OBJECTIVES: A previous study indicated that the extension of whole blood (WB) storage from 8 to 24 h at 20-24 degrees C before the processing of platelet-rich plasma (PRP)-depleted red blood cell (RBC) units had a negative effect on the efficacy of leucoreduction filters. In this study, we ...
Bu Ying - - 2008
We described a novel approach to directly amplify genomic DNA from whole blood and dried blood spotted on filter paper without any DNA isolation by using the PCR buffer with a higher pH, which was optimized as pH 9.1-9.6. Direct PCR on blood treated with various anticoagulants showed that the ...
Mlambo Godfree - - 2008
Plasmodium falciparum gametocytes are obligate parasite sexual stages required for transmission of malaria from human hosts to the mosquito vector. Assessment of gametocyte carriers in the population is critical in understanding malaria transmission dynamics and in epidemiology studies. We applied a reverse transcription-polymerase chain reaction (RT-PCR)-based approach to detect pfs25 ...
MacNutt Meaghan J - - 2008
Researchers and clinicians may not be aware that the performance of evacuated blood collection systems is impaired at high altitude. We tested four sizes of Vacutainer tubes at altitudes ranging from sea level to 5341 m to and determined that draw volumes are reduced by approximately 0.5 mL for every ...
Dresse Marie - - 2008
BACKGROUND: Tissue inhibitor of metalloproteinases-1 (TIMP-1) in blood might be a helpful biomarker in various diseases. However, various authors report that TIMP-1 is dependent on preanalytical procedures. Our study was performed to determine how storage conditions and time to centrifugation influence TIMP-1. MATERIALS AND METHODS: Twenty-six blood specimens were collected ...
Wilkes A R - - 2008
The effect of flow on the filtration performance of six different types of filter intended for use in paediatric anaesthesia was measured by challenging the filters with sodium chloride particles at five different flows: 6, 10, 15, 20 and 30 l x min(-1). Twenty-five unused samples of each filter type ...
Tangvarasittichai Orathai - - 2008
The present study was undertaken to assess the feasibility of whole dried blood spots to identify alpha-thalassemia-1 by PCR technique. Logistical constraints associated with sample collection and processing make the measurement of alpha-thalassemia-1 at community laboratories difficult. Dried blood spot samples provide a relatively convenient and minimally invasive means for ...
Lomeo Angelo - - 2008
To simplify collection and transport of blood for HbA1c measuring, we have studied the use of a special paper that absorbs a defined volume of capillary blood and quickly dries it (dried blood-spot, DBS). The DBS can be sent to a central laboratory using regular postal service and without temperature ...
Corran Patrick H - - 2008
BACKGROUND: Blood spots collected onto filter paper are an established and convenient source of antibodies for serological diagnosis and epidemiological surveys. Although recommendations for the storage and analysis of small molecule analytes in blood spots exist, there are no published systematic studies of the stability of antibodies under different storage ...
Elrayah, I. E.
A survey was conducted in a low-endemic and in a non-endemic area of Sudan to evaluate the specificity and efficiency of different serological antibody detection techniques for Trypanosoma brucei gambiense. Comparisons were made of the card agglutination test for trypanosomiasis (CATT) on diluted blood, on diluted plasma and on eluates ...
Braun R K - - 2007
Flow cytometry has evolved from single- and two-color analysis to the current use of 11-16 colors. The relatively bright excitation spectra of most fluorochromes have made color compensation a challenge especially when performed manually. We describe how by choosing filters with narrower bandwidths results in the color compensation values between ...
Okano Yoshiyuki - - 2007
BH(4) administration results in the reduction of blood phenylalanine level in patients with tetrahydrobiopterin (BH(4))-responsive phenylalanine hydroxylase (PAH) deficiency. The mechanism underlying BH(4) response remains unknown. Here, we studied the effects of BH(4) and phenylalanine on in vivo PAH activity of normal controls using the phenylalanine breath test (PBT) by ...
Ferrarotti Ilaria - - 2007
The laboratory diagnosis of alpha(1)-antitrypsin (AAT) deficiency (AATD) has evolved over the last 40 years since the first cases of the disorder were reported. It is currently performed in specialized centers, and it requires a combination of different biochemical methods: nephelometric AAT concentration, isoelectric focusing, genotyping, and sequencing. The availability ...
Elrayah I E - - 2007
A survey was conducted in a low-endemic and in a non-endemic area of Sudan to evaluate the specificity and efficiency of different serological antibody detection techniques for Trypanosoma brucei gambiense. Comparisons were made of the card agglutination test for trypanosomiasis (CATT) on diluted blood, on diluted plasma and on eluates ...
Padilla Carmencita D - - 2007
The success of blood spot newborn screening in the USA led to early screening efforts in parts of the Asia Pacific Region in the mid-1960s. While there were early screening leaders in the region, many of the countries with depressed and developing economies are only now beginning organized screening efforts. ...
Chen Grace P GP Division of Cardiology, Department of Medicine, University of Washington, Seattle, Washington, - - 2007
Filtered backprojection (FBP) is the traditional method for 13N-NH3 PET studies. Ordered-subsets expectation maximization (OSEM) is popular for PET studies because of better noise properties. Scant data exist on the effect of reconstruction algorithms on quantitative myocardial blood flow (MBF) estimation. Twenty patients underwent dynamic acquisition rest/stress 13N-NH3 studies. In ...
Yao Maosheng - - 2007
Portable impactors are increasingly being used to estimate concentration of bioaerosols in residential and occupational environments; however, little data are available about their performance. This study investigated the overall performances of the SMA MicroPortable, BioCulture, Microflow, Microbiological Air Sampler (MAS-100), Millipore Air Tester, SAS Super 180, and RCS High Flow ...
Elvers L H - - 2007
BACKGROUND: Neonatal screening for congenital disorders like phenylketonuria (PKU), congenital hypothyroidism (CH) and congenital adrenal hyperplasia (CAH) is generally performed in dried blood spots on filter paper. The analytes of interest for testing for PKU, CH and CAH are phenylalanine, thyrotropin (TSH) and 17alpha-hydroxyprogesterone (17OHP), respectively. The International Society for ...
Wiltbank Thomas B - - 2007
BACKGROUND: Recent technology allows for the collection of 2-unit red cells (RBCs) and single-unit RBCs plus plasma or platelets (PLTs). STUDY DESIGN AND METHODS: With a common definition of adverse events, 1,023,682 whole-blood collections were evaluated and compared with 249,154 two-unit apheresis RBC collections, 40,870 single-apheresis RBC collections, and 90,082 ...
Tanner Susan - - 2007
Elevated circulating levels of total immunoglobulin E (IgE) are associated with both allergic disease and repeated macro-parasitic infections. Population-based research on IgE has been limited by the logistical constraints associated with obtaining and processing venipuncture blood samples. In this short report, we present an enzyme immunoassay protocol for quantifying circulating ...
Sellam Jérémie - - 2007
Latent tuberculosis infection (LTBI) is detected with the tuberculin skin test (TST) before anti-TNF therapy. We aimed to investigate in vitro blood assays with TB-specific antigens (CFP-10, ESAT-6), in immune-mediated inflammatory diseases (IMID) for LTBI screening. Sixty-eight IMID patients with (n = 35) or without (n = 33) LTBI according ...
Cizdziel James V - - 2007
Screening programs for pediatric lead (Pb) poisoning routinely collect blood by finger stick onto specially designed filter paper (FP) for subsequent laboratory testing. Here, laser ablation inductively coupled plasma time-of-flight mass spectrometry is evaluated as a novel method for the determination of Pb in blood spotted onto FP cards. The ...
Pinkerton P H - - 2007
BACKGROUND AND OBJECTIVES: In an attempt to determine how much scope there might be for blood conservation in Canada, the population based rate of blood collection was compared to that in other countries. MATERIALS AND METHOD: Blood collections and population size in 18 countries were obtained from national blood programs ...
Trudeau S - - 2007
Birds of prey that are poisoned by cholinesterase inhibitors (e.g. organophosphate and carbamate insecticides) are often cared for at animal shelters, rehabilitation centres and wildlife diagnostic facilities. Plasma cholinesterase (ChE) activity is a recognized method of assessing exposure to these insecticides, but standard blood-handling protocols are difficult to follow in ...
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