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Results 251 - 300 of 862
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MacNutt Meaghan J - - 2008
Researchers and clinicians may not be aware that the performance of evacuated blood collection systems is impaired at high altitude. We tested four sizes of Vacutainer tubes at altitudes ranging from sea level to 5341 m to and determined that draw volumes are reduced by approximately 0.5 mL for every ...
Dresse Marie - - 2008
BACKGROUND: Tissue inhibitor of metalloproteinases-1 (TIMP-1) in blood might be a helpful biomarker in various diseases. However, various authors report that TIMP-1 is dependent on preanalytical procedures. Our study was performed to determine how storage conditions and time to centrifugation influence TIMP-1. MATERIALS AND METHODS: Twenty-six blood specimens were collected ...
Wilkes A R - - 2008
The effect of flow on the filtration performance of six different types of filter intended for use in paediatric anaesthesia was measured by challenging the filters with sodium chloride particles at five different flows: 6, 10, 15, 20 and 30 l x min(-1). Twenty-five unused samples of each filter type ...
Tangvarasittichai Orathai - - 2008
The present study was undertaken to assess the feasibility of whole dried blood spots to identify alpha-thalassemia-1 by PCR technique. Logistical constraints associated with sample collection and processing make the measurement of alpha-thalassemia-1 at community laboratories difficult. Dried blood spot samples provide a relatively convenient and minimally invasive means for ...
Lomeo Angelo - - 2008
To simplify collection and transport of blood for HbA1c measuring, we have studied the use of a special paper that absorbs a defined volume of capillary blood and quickly dries it (dried blood-spot, DBS). The DBS can be sent to a central laboratory using regular postal service and without temperature ...
Corran Patrick H - - 2008
BACKGROUND: Blood spots collected onto filter paper are an established and convenient source of antibodies for serological diagnosis and epidemiological surveys. Although recommendations for the storage and analysis of small molecule analytes in blood spots exist, there are no published systematic studies of the stability of antibodies under different storage ...
Elrayah, I. E.
A survey was conducted in a low-endemic and in a non-endemic area of Sudan to evaluate the specificity and efficiency of different serological antibody detection techniques for Trypanosoma brucei gambiense. Comparisons were made of the card agglutination test for trypanosomiasis (CATT) on diluted blood, on diluted plasma and on eluates ...
Braun R K - - 2007
Flow cytometry has evolved from single- and two-color analysis to the current use of 11-16 colors. The relatively bright excitation spectra of most fluorochromes have made color compensation a challenge especially when performed manually. We describe how by choosing filters with narrower bandwidths results in the color compensation values between ...
Okano Yoshiyuki - - 2007
BH(4) administration results in the reduction of blood phenylalanine level in patients with tetrahydrobiopterin (BH(4))-responsive phenylalanine hydroxylase (PAH) deficiency. The mechanism underlying BH(4) response remains unknown. Here, we studied the effects of BH(4) and phenylalanine on in vivo PAH activity of normal controls using the phenylalanine breath test (PBT) by ...
Ferrarotti Ilaria - - 2007
The laboratory diagnosis of alpha(1)-antitrypsin (AAT) deficiency (AATD) has evolved over the last 40 years since the first cases of the disorder were reported. It is currently performed in specialized centers, and it requires a combination of different biochemical methods: nephelometric AAT concentration, isoelectric focusing, genotyping, and sequencing. The availability ...
Elrayah I E - - 2007
A survey was conducted in a low-endemic and in a non-endemic area of Sudan to evaluate the specificity and efficiency of different serological antibody detection techniques for Trypanosoma brucei gambiense. Comparisons were made of the card agglutination test for trypanosomiasis (CATT) on diluted blood, on diluted plasma and on eluates ...
Padilla Carmencita D - - 2007
The success of blood spot newborn screening in the USA led to early screening efforts in parts of the Asia Pacific Region in the mid-1960s. While there were early screening leaders in the region, many of the countries with depressed and developing economies are only now beginning organized screening efforts. ...
Chen Grace P - - 2007
Filtered backprojection (FBP) is the traditional method for 13N-NH3 PET studies. Ordered-subsets expectation maximization (OSEM) is popular for PET studies because of better noise properties. Scant data exist on the effect of reconstruction algorithms on quantitative myocardial blood flow (MBF) estimation. Twenty patients underwent dynamic acquisition rest/stress 13N-NH3 studies. In ...
Yao Maosheng - - 2007
Portable impactors are increasingly being used to estimate concentration of bioaerosols in residential and occupational environments; however, little data are available about their performance. This study investigated the overall performances of the SMA MicroPortable, BioCulture, Microflow, Microbiological Air Sampler (MAS-100), Millipore Air Tester, SAS Super 180, and RCS High Flow ...
Elvers L H - - 2007
BACKGROUND: Neonatal screening for congenital disorders like phenylketonuria (PKU), congenital hypothyroidism (CH) and congenital adrenal hyperplasia (CAH) is generally performed in dried blood spots on filter paper. The analytes of interest for testing for PKU, CH and CAH are phenylalanine, thyrotropin (TSH) and 17alpha-hydroxyprogesterone (17OHP), respectively. The International Society for ...
Wiltbank Thomas B - - 2007
BACKGROUND: Recent technology allows for the collection of 2-unit red cells (RBCs) and single-unit RBCs plus plasma or platelets (PLTs). STUDY DESIGN AND METHODS: With a common definition of adverse events, 1,023,682 whole-blood collections were evaluated and compared with 249,154 two-unit apheresis RBC collections, 40,870 single-apheresis RBC collections, and 90,082 ...
Tanner Susan - - 2007
Elevated circulating levels of total immunoglobulin E (IgE) are associated with both allergic disease and repeated macro-parasitic infections. Population-based research on IgE has been limited by the logistical constraints associated with obtaining and processing venipuncture blood samples. In this short report, we present an enzyme immunoassay protocol for quantifying circulating ...
Sellam Jérémie - - 2007
Latent tuberculosis infection (LTBI) is detected with the tuberculin skin test (TST) before anti-TNF therapy. We aimed to investigate in vitro blood assays with TB-specific antigens (CFP-10, ESAT-6), in immune-mediated inflammatory diseases (IMID) for LTBI screening. Sixty-eight IMID patients with (n = 35) or without (n = 33) LTBI according ...
Cizdziel James V - - 2007
Screening programs for pediatric lead (Pb) poisoning routinely collect blood by finger stick onto specially designed filter paper (FP) for subsequent laboratory testing. Here, laser ablation inductively coupled plasma time-of-flight mass spectrometry is evaluated as a novel method for the determination of Pb in blood spotted onto FP cards. The ...
Pinkerton P H - - 2007
BACKGROUND AND OBJECTIVES: In an attempt to determine how much scope there might be for blood conservation in Canada, the population based rate of blood collection was compared to that in other countries. MATERIALS AND METHOD: Blood collections and population size in 18 countries were obtained from national blood programs ...
Trudeau S - - 2007
Birds of prey that are poisoned by cholinesterase inhibitors (e.g. organophosphate and carbamate insecticides) are often cared for at animal shelters, rehabilitation centres and wildlife diagnostic facilities. Plasma cholinesterase (ChE) activity is a recognized method of assessing exposure to these insecticides, but standard blood-handling protocols are difficult to follow in ...
Kutschka Ingo - - 2007
Minimized perfusion circuits (MPCs) have been criticized for insufficient air elimination. The deairing capabilities of a new MPC, including an ultrasound controlled deairing unit, were compared to a standard extracorporeal circuit (ECC) in a laboratory setup. During blood flow of 4.0l/min, we injected 30-cc air over a period of 30 ...
Anjali - - 2007
BACKGROUND AND OBJECTIVES: Glycated hemoglobin (HbA1c) provides an accurate and reliable method to assess the glycemic control in patients with Diabetes. Its measurement is limited by the inconvenience of sample collection that requires venipuncture, sample handling and storage factors. The aim of this study was to assess the feasibility of ...
Ayele Workenesh - - 2007
We studied the use of dried spots of bodily fluids (plasma, whole blood, and mother's milk) on filter paper as a means of sample collection and storage for human immunodeficiency virus type 1 (HIV-1) viral load testing under stringent field conditions. Plasma placed directly in lysis buffer, which is customarily ...
Handali Sukwan - - 2007
Compliance and acceptance for the finger-prick method of blood collection is generally better than for venipuncture. A finger-prick method of blood collection with quantitative antibody recovery is even more important for seroepidemiological surveys. Finger-prick blood collected and dried onto filter paper has been used; but, unfortunately, this method has several ...
Maucher Jennifer M - - 2007
Blood collection cards have been successfully used as a tool to monitor brevetoxin (PbTx) exposure in several species, including fish, mice, and rats. Previous methanolic methods used for extracting brevetoxin from blood collection cards have shown dolphin blood to have matrix difficulties in several biological assays. To better biomonitor protected ...
Fekkes Durk - - 2007
OBJECTIVE: To develop a reliable method for the determination of tetrahydrobiopterin and total biopterin in plasma. METHODS: Modifications include blood collection and handling procedures. RESULTS: Collection of blood in the presence of 0.1% (w/v) dithiothreitol and waiting for 2-3 h before centrifugation are optimal for adequate quantitation of both pterins. ...
Rustige H - - 2007
A three-stage constructed wetland for leachate treatment was monitored on a landfill at a pilot scale. The plant had been designed to achieve at least 75% nitrogen removal. NH4-N input concentration was 240 (median) up to 290 mgl(-1) and COD concentration was 455 to 511 mgl(-1), respectively. A 14 m2 ...
Hamblin Mark N - - 2007
Electroosmotic flow was studied in thin film microchannels with silicon dioxide and silicon nitride sidewalls formed using plasma-enhanced chemical vapor deposition (PECVD). A sacrificial etching process was employed for channel fabrication allowing for cross-sections with heights of 3 mum, ranging from 2 mum to 50 mum in width. Flow rates ...
Fan Yun-Hua - - 2007
Glucose-6-phosphate dehydrogenase (G6PD) deficiency is a common hereditary enzyme defect that affects over 400 million people worldwide. Deficiency in G6PD can cause hemolytic anemia, which can be fatal if it is not treated properly. Mass qualitative G6PD-deficiency screening methods have been available for some time; however, in this study we ...
Arifin Dian R - - 2007
An effective mechanism for rapid and efficient microfluidic particle trapping and concentration is proposed without requiring any mechanically moving parts. When a voltage beyond the threshold atmospheric ionization value is applied on a sharp electrode tip mounted at an angle above a microfluidic liquid chamber, the bulk electrohydrodynamic air thrust ...
Felberova L - - 2007
Among the most common non-conventional wastewater treatment techniques used in the Czech Republic are waste stabilisation ponds (WSP), subsurface horizontal flow constructed wetlands (CW) and vertical flow groundfilters (GF). These extensive systems can be advantageously used for treatment of waters coming from sewerages where the ballast weighting commonly makes more ...
Wang Danping - - 2007
Two micro fibre optic flow checkers are presented in this paper. They are used for a medical analysis to control a solvent flow up to 1microl/min resolution. A fibre optic sensor as well as a hydraulic system are the principle components of these flow checkers. This paper describes the principle ...
Mannion Heidi - - 2007
OBJECTIVE: This study was designed to compare the effectiveness of three alternative structural configurations for inpatient phlebotomy. It was hypothesized that decentralized was less effective when compared to centralized inpatient phlebotomy. DESIGN: A non-experimental prospective survey design was conducted at the institution level. Laboratory managers completed an organizational survey and ...
Edwards John M - - 2007
Electro-osmotic flow (EOF) pumps are attractive for fluid manipulation in microfluidic channels. Open channel EOF pumps can produce high pressures and flow rates, and are relatively easy to fabricate on-chip or integrate with other microfluidic or electrical components. An EOF pump design that is conducive to on-chip fabrication consists of ...
Mafham Marion M MM Renal Studies Group, Clinical Trial Service Unit, Old Road Campus, Oxford, UK. - - 2007
Exogenous tracer-based methods of measuring glomerular filtration rate (GFR) are difficult to perform, whilst creatinine-based estimation formulae are inaccurate. We assessed a new technique of measuring iohexol clearance using timed dried capillary blood spots. A reference GFR was measured in 81 subjects (GFR 15-124 ml/min/1.73 m(2)) by iohexol clearance using ...
Inoue Ryo - - 2007
The combination of Flinders Technology Associates filter papers (FTA cards) and real-time PCR was examined to establish a simple and rapid technique for the detection of porcine reproductive and respiratory syndrome virus (PRRSV) from whole pig blood. A modified live PRRS vaccine was diluted with either sterilised saline or pig ...
Hoogtanders K - - 2007
In a preliminary investigation an assay for tacrolimus based on fingerprick sampling and consecutive application as a blood spot on sampling paper has been developed. The dried blood spot was analysed by HPLC-tandem mass spectrometry. The validated range was 1-30 microg/l. Intra- and inter-assay variability for precision and accuracy was ...
Schultz Melissa M - - 2006
Fluorochemicals have widespread applications and are released into municipal wastewater treatment plants via domestic wastewater. A field study was conducted at a full-scale municipal wastewater treatment plant to determine the mass flows of selected fluorochemicals. Flow-proportional, 24 h samples of raw influent, primary effluent, trickling filter effluent, secondary effluent, and ...
Noorvee Alar - - 2007
Different conditions (water level, oxygen supply) prevailing in both beds of the Kodijärve double-bed horizontal subsurface flow (HSSF) constructed wetland (CW) (Southern Estonia; constructed in 1996, total area 312.5 m(2), 40 pe) provide the opportunity to compare how different operational methods have altered the efficiency of the purification processes inside ...
Strasser Stefan - - 2007
We present a new tool for the estimation of the age of bloodstains, which could probably be used during forensic casework. For this, we used atomic force microscopy (AFM) for high-resolution imaging of erythrocytes in a blood sample and the detection of elasticity changes on a nanometer scale. For the ...
Cardo, Lisa J.
BACKGROUND: Leishmania is an intracellular parasite of monocytes transmissible by transfusion. The feasibility of reducing Leishmania with leukodepletion filters was studied. At collection, infected blood contains the amastigote form of Leishmania within monocytes. Amastigotes cause the rupture of monocytes releasing free amastigotes that convert to promastigotes which exist extracellularly at ...
Brandl M - - 2006
In the extracorporeal adsorption system, MDS (Microspheres based Detoxification System), micro-adsorbent particles measuring 1-25 micrometers circulate in a filtrate circuit for highly specific blood purification/adsorption. The MDS circuit containing the adsorbent microparticles is linked to the patient's blood line by a hollow fiber plasma filter. When the transmembrane pressure or ...
Miller Aaron A - - 2006
Leptin is important in a wide range of physiological processes, but logistical constraints associated with venipuncture blood collection have limited research on leptin in diverse, community-based settings. The aim of this short report is to present and validate an enzyme immunoassay method for quantifying leptin in samples of capillary whole ...
Blanco-Canqui Humberto - - 2006
Effectiveness of grass barriers and vegetative filter strips (FS) for reducing transport of sediment and nutrients in runoff may depend on runoff flow conditions. We assessed the performance of (1) switchgrass (Panicum virgatum L.) barriers (0.7 m) planted above fescue (Festuca arundinacea Schreb.) filter strips under interrill (B-FS) and concentrated ...
Bottein Dechraoui Marie-Yasmine - - 2007
Ciguatera diagnosis relies on clinical observations associated with a recent consumption of fish. Although needed, direct confirmation of exposure in subjects showing ciguatera disease symptoms is currently unavailable. We previously reported that ciguatoxins were measurable in the blood of mice exposed to extracts of Pacific ciguatoxins isolated from Gambierdiscus polynesiensis, ...
Alonso-Echanove Juan - - 2006
OBJECTIVE: To characterize red eye reactions occurring within 24 hours after receipt of units of leukocyte-reduced red blood cells, determine their etiology, and investigate their potential link to transfusion. METHODS: We conducted a survey of transfusion facilities nationwide to determine the scope and magnitude of the reactions; performed case-control and ...
Aksu Zümriye - - 2007
A continuous fixed bed study was carried out by using dried Rhizopus arrhizus as a biosorbent for the removal of three reactive dyes; Gemacion (Procion) Red H-E7B (GR), a monoclorotriazine mono-azo type reactive dye; Gemazol Turquise Blue-G (GTB), a vinyl sulfone mono-azo type reactive dye and Gemactive (Reactive) Black HFGR ...
Sagart, Laurent
This paper argues that the Chinese word for 'blood', Middle Chinese hwit, had initial hm- in Old Chinese.
Chaturvedi Sujata - - 2006
Blood collection is an important preanalytical component of haematological testing. This questionnaire based study was conducted on laboratory personnel to elicit their knowledge and practice regarding blood collection procedures for haematological testing. Questionnaire comprised of 37 multiple choice questions, of which 10 questions each were related to essential and desirable ...
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