Search Results
Results 201 - 250 of 892
< 1 2 3 4 5 6 7 8 9 10 >
Duffey Trisha A TA Department of Chemistry, University of Washington, Seattle, Washington 98195, United - - 2010
We report a new assay of N-acetylgalactosamine-4-sulfatase (aryl sulfatase B) activity in dried blood spots (DBS) for the early detection of mucopolysaccharidosis VI (Maroteaux-Lamy syndrome) in newborn screening. The assay uses a synthetic substrate consisting of N-acetylgalactosamine-4-sulfate moiety glycosidically linked to a hydrophobic residue and furnished with a tert-butyloxycarbamido group ...
Drescher Knut - - 2010
Swimming microorganisms create flows that influence their mutual interactions and modify the rheology of their suspensions. While extensively studied theoretically, these flows have not been measured in detail around any freely-swimming microorganism. We report such measurements for the microphytes Volvox carteri and Chlamydomonas reinhardtii. The minute (∼0.3%) density excess of ...
Wang Jun J NanoSystems Biology Cancer Center, California Institute of Technology, Pasadena, CA 91125, - - 2010
We describe an automated, self-powered chip based on lateral flow immunoassay for rapid, quantitative, and multiplex protein detection from pinpricks of whole blood. The device incorporates on-chip purification of blood plasma by employing inertial forces to focus blood cells away from the assay surface, where plasma proteins are captured and ...
Sorimachi Takatoshi - - 2010
To determine any relationship between angiographically documented flow impairment associated with the Angioguard XP cerebral protection filter during carotid artery stenting (CAS) and the condition of the pores in the retrieved filter. To obtain baseline characteristics of the Angioguard XP, an unused filter was fully expanded and examined photographically from ...
Messinger R J - - 2010
We show that nanoscale surface roughness, which commonly occurs on microfabricated metal electrodes, can significantly suppress electro-osmotic flows when excess surface conductivity is appreciable. We demonstrate the physical mechanism for electro-osmotic flow suppression due to surface curvature, compute the effects of varying surface conductivity and roughness amplitudes on the slip ...
Sander Johannes - - 2011
Quantification of nitisinone, 2-(nitro-4-trifluoromethylbenzoyl)1,3-cyclohexanedione (NTBC) has been repeatedly described. Nevertheless monitoring of NTBC has not yet become part of routine therapy surveillance in tyrosinaemia type I (OMIM 276700). We developed a blood spot test to facilitate collection and transport of samples. Furthermore, the test material can be used for determination ...
Yamada Chisa - - 2010
Hyperkalemia is a serious complication of rapid and massive blood transfusion due to high plasma potassium (K) in stored red blood cell (RBC) units. A potassium adsorption filter (PAF) was developed in Japan to remove K by exchanging with sodium (Na). We performed an in vitro evaluation of its efficacy ...
Zhu Taishan - - 2010
The presented work probes the fundamentals of Knudsen forces. Using the direct simulation Monte Carlo (DSMC) method, the flows induced by temperature inhomogeneity within a representative configuration and the Knudsen force acting on a heated microbeam are captured as functions of Knudsen number in the entire flow regime. Both flow ...
Karinen Ritva - - 2010
The aim of this study was to evaluate the stability of blood concentrations of a variety of illegal and medicinal drugs that are important for forensic analyses when spiked and stored in Vacutainer or Venosafe evacuated plastic collection tubes compared to Vacutainer evacuated glass tubes. Tubes were filled with spiked ...
Sharma Mradul - - 2010
The transport of angular momentum in the outward direction is the fundamental requirement for accretion to proceed in an accretion disk. This objective can be achieved if the accretion flow is turbulent. Instabilities are one of the sources for the turbulence. We study a differentially rotating compressive flow in the ...
Liu Jia - - 2010
Cerebral autoregulation has been studied by linear filter systems, with arterial blood pressure (ABP) as the input and cerebral blood flow velocity (CBFV-from transcranial Doppler Ultrasound) as the output. The current work extends this by using adaptive filters to investigate the dynamics of time-varying cerebral autoregulation during step-wise changes in ...
Karlo Christoph - - 2011
To differentiate proxy renal cystic lesions containing protein, blood, iodine contrast or saline solutions using dual-energy CT (DECT) equipped with a new tin filter technology (TFT). 70 proxies (saline, protein, blood and contrast agent) were placed in unenhanced and contrast-enhanced kidney phantoms. DECT was performed at 80/140 kV with and without ...
Salavitabar Arash - - 2010
Cardiopulmonary bypass (CPB) can be a potential cause of morbidity in patients for several reasons, including significantly higher gaseous microemboli (GME) formation than extracorporeal life support (ECLS) and physiological circulation, diverted blood flow from the patient via an open purge line of the arterial filter, and pressure drop across the ...
Ren Xiao - - 2010
Dried blood spot (DBS) sampling could potentially become the preferred blood collection technique in toxicological and clinical studies. Autoradiography was performed to study compound distribution within a dbs under different conditions using five papers, 31ETF, Grade 226, 903(®), FTA(®) and FTA(®) Elute. The results showed an uneven distribution in all ...
Mei Joanne V - - 2010
The Newborn Screening Quality Assurance Program at the Centers for Disease Control and Prevention assesses the adherence to established performance standards of manufactured lots of whole blood filter paper collection devices that are registered by the US FDA. We examined 26 newborn screening analytes measured from blood applied to filter ...
El Ezzi Asmahan A - - 2010
Congenital hypothyroidism is screened using blood spotted on filter paper that may be transported from remote areas to central testing facilities. However, storage conditions and transportation may affect sample quality. We examined long-term stability of thyroid-stimulating hormone (TSH) and thyroxin (TT4) in blood spotted on filter paper, which was stored ...
Kauffman Peter - - 2010
The two-dimensional paper network (2DPN) is a versatile new microfluidic format for performing complex chemical processes. For chemical detection, for example, 2DPNs have the potential to exceed the capabilities and performance of existing paper-based lateral flow devices at a comparable cost and ease of use. To design such 2DPNs, it ...
Kim Jungkyu - - 2010
Biomolecules, including protein A, albumin, and immunoglobulin G, are spotted on top of a nanoporous substrate by using a continuous-flow microspotter (CFM) system, which normally produces spots 3 to 4 orders of magnitude more sensitive than conventional biomolecule printing methods. The spots are observed with a fluorescence scanner. By using ...
Liebers V - - 2010
Organic dust is composed of numerous components. Exposed people may have health problems. Respiratory tract complaints may occur at workplaces like those in agriculture or in the waste industry. Therefore methods that determine the composition and activity of dust components are important. They help in the performance of risk estimations ...
Rowland Malcolm - - 2010
Dried blood spots are increasingly being used in drug development. This commentary considers the pharmacokinetic issues that arise and compares these with those attached to plasma, the mainstay matrix. A common implicit use of these matrices is as a surrogate for plasma water, and to this extent, the critical assumption ...
Klemencic Stephanie - - 2010
BACKGROUND: The disease-modifying antirheumatic drug methotrexate (MTX) is the treatment of choice for many patients with rheumatoid arthritis. Systemic toxicity in MTX use is well documented. Side effects such as pancytopenia, myelosuppression, hepatotoxicity, and pulmonary toxicity may be life-threatening. Various ocular effects with methotrexate use have been reported; however, to ...
Jones Trevor G - - 2010
Hemoglobin A1c (HbA1c) has been endorsed as a tool for the diagnosis of diabetes. This test requires instrumentation that may not be available in underdeveloped areas. Dried blood spot (DBS) samples collected by finger stick procedures offer a mechanism to transport samples to laboratories that do measure HbA1c. Whole blood ...
Lakshmy Ramakrishnan - - 2010
Developing countries are facing a rise in noncommunicable diseases (NCD), which is a cause for concern. The World Health Organization has recommended a stepwise approach for NCD risk factor surveillance. Screening for risk factors in remote populations is difficult due to lack of resources and technical expertise, including standardized laboratory ...
Cho Sung Hwan - - 2010
There is an increasing need to develop optofluidic flow cytometers. Optofluidics, where optics and microfluidics work together to create novel functionalities on a small chip, holds great promise for lab-on-a-chip flow cytometry. The development of a low-cost, compact, handheld flow cytometer and microfluorescence-activated cell sorter system could have a significant ...
Alves Crésio - - 2010
The evaluation of the salivary flow rate is important in several situations of dental practice. However, weighing the collected salivary volume it is not a practical method in public health services. This work evaluated the use of hypodermic graduated syringes as a simpler alternative method to measure the salivary flow ...
Persson Anders - - 2010
To evaluate the performance of dried blood spots (DBSs) with subsequent analyses of glutamic acid decarboxylase (GADA) and islet antigen-2 (IA-2A) with the RSR-ELISAs, we selected 80 children newly diagnosed with type 1 diabetes and 120 healthy women. DBSs from patients and controls were used for RSR-ELISAs while patients samples ...
Sreelakshmi T R - - 2010
Summary Intra-operative cell salvage is used in more than 75% of NHS hospitals in the United Kingdom and is a safe and cost effective alternative to allogenic blood transfusion. We report a case of acute hypotension during reinfusion of cell salvaged blood through a leucocyte depletion filter that occurred during ...
Rissler Jenny - - 2009
BACKGROUND: Mass-weighted aerodynamic particle-size distribution (APSD) is a key attribute for pharmaceutical products developed to deliver drugs to or through the lungs. In development and quality control, APSD is primarily determined using multistage cascade impactors. For impactor techniques, particle reentrainment is critical because it may lead to an overestimation of ...
Wiltshire Michael - - 2010
BACKGROUND: A filter has been developed (P-Capt, MacoPharma) to remove infectious prions from red blood cells (RBCs). We sought to assess 1) its operational use, 2) the quality of filtered components, and 3) whether filtration resulted in any significant changes to blood group antigens. STUDY DESIGN AND METHODS: A total ...
Figueredo-Cardero Alvio - - 2009
In the present work Computational Fluid Dynamics (CFD) was used to study the flow field and particle dynamics in an internal spin-filter (SF) bioreactor system. Evidence of a radial exchange flow through the filter mesh was detected, with a magnitude up to 130-fold higher than the perfusion flow, thus significantly ...
Hussaini Bader E - - 2009
BACKGROUND: Biocompatible surfaces play an important role in the inflammatory response during cardiopulmonary bypass (CBP), with the arterial filter contributing a large surface area of the circuit. Different filter-coating materials designed to improve blood-filter biocompatibility are currently used in CPB circuits. This study evaluates eight biocompatible coatings used for arterial ...
Lakshmy Ramakrishnan - - 2009
Glycated hemoglobin A1c (A1C) is an important marker in the diagnosis and treatment of diabetes. Dried blood measurement of A1C is useful in large scale epidemiological evaluation of A1C, especially to assess the impact of intervention programs. The possibility of using dried blood for measurement of A1C by the immunoturbidimetric ...
Cho Kyungmin Jacob - - 2010
The aim of this study was to investigate respirator filter and faceseal penetration of particles representing bacterial and fungal spore size ranges (0.7-4 mum). First, field experiments were conducted to determine workplace protection factors (WPFs) for a typical N95 filtering facepiece respirator (FFR). These data (average WPF = 515) were ...
Glenister Kristen M - - 2010
BACKGROUND: In contrast to the well-documented effect of white blood cells on the quality of red blood cells (RBCs), the effect of platelets (PLTs) has received little consideration. In this study, the PLT content and level of PLT-derived cytokines in RBCs prepared using different types of leukoreduction methods were investigated. ...
Whitney James B - - 2009
The ability to detect and quantify acute HIV-1 infection prior to seroconversion would be an important tool for use in HIV vaccine clinical efficacy trials. We have utilized the SIV/rhesus monkey model to evaluate whether samples more easily obtained than peripheral blood might be used for intensive monitoring of vaccine ...
Gulliksson Hans - - 2009
Routines for the storage of whole blood (WB) overnight for the preparation of blood components on the following day are of increasing interest primarily for logistic reasons. The present study focuses on in vitro effects during storage for 6 weeks on red blood cells (RBC) prepared in different blood containers ...
Tan Hooi-Kuan - - 2009
Retrospective testing of neonatal Guthrie card blood spots for specific immunoglobulin M (IgM) can distinguish congenital toxoplasmosis from acquired toxoplasmosis. We determined whether storage temperature reduced IgM detection, using filter paper blood samples "spiked" with anti-Toxoplasma IgM. After 300 days, IgM detection deteriorated with storage at room temperature but not ...
Singer Michael A - - 2009
PURPOSE: To evaluate the hemodynamics of the TrapEase vena cava filter (Cordis, Miami Lakes, Florida) by using three-dimensional computational fluid dynamics, including simulated thrombi of multiple shapes, sizes, and trapping positions. The study was performed to identify areas of stagnant and/or recirculating flow that may have an effect on intrafilter ...
Phetsouvanh Rattanaphone - - 2009
We performed indirect immunofluorescence assays (IFAs) to compare levels of IgM and IgG antibodies to Orientia tsutsugamushi and Rickettsia typhi in admission-phase serum samples and filter paper blood spots (assayed immediately and stored at 5.4 degrees C and 29 degrees C for 30 days) collected on the same day from ...
Qin Lidong L NanoSystems Biology Cancer Center(NSBCC), Kavli Nanoscience Institute, Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, - - 2009
We report herein on a self-powered, self-contained microfluidic-based chip designed to separate plasma from whole blood, and then execute an assay of a multiplexed panel of plasma biomarker proteins. The power source is based upon a chemical reaction that is catalytically triggered by the push of a button on the ...
Johnson Michel J - - 2009
During Doppler ultrasound assessment of blood flow, a wall filter is used to reduce or eliminate high-amplitude, low-velocity signals from the vessel wall and the surrounding tissue. This study investigated the impact of a range of wall filters (22 Hz, 75 Hz, 128 Hz, and 252 Hz) on the accuracy ...
Ricci Zaccaria - - 2009
We wanted to evaluate if brain natriuretic peptide (BNP) is cleared during continuous veno-venous hemofiltration (CVVH) sessions in children with congenital heart disease. A prospective observational single-center study was conducted in a post-cardiac surgery intensive care unit of the city children's hospital. Ten children requiring CVVH for acute kidney injury ...
Myers Gerard J - - 2009
During a previously published study on gaseous microemboli (GMEs) and perfusionist interventions, it was noted that emboli could be detected after the arterial filter when blood/air challenges entered the membrane oxygenator's integral cardiotomy. The findings indicated that further study into the oxygenator's integral cardiotomy reservoir was warranted. This is the ...
Nugent C Thomas - - 2009
Peripheral blood mononuclear cells (PBMCs), saliva, seminal plasma, and dried blood spots were evaluated as specimen types for the APTIMA HIV-1 RNA Qualitative Assay (APTIMA HIV-1 Assay), which employs a target capture step to recover HIV-1-specific sequences from complex specimen types. Analytical sensitivity studies were carried out using samples that ...
Duscher Georg - - 2009
Storage of blood samples for subsequent DNA extraction without loss of integrity can be difficult under field conditions. Filter-based technologies are known to deliver good results for viral, bacterial and protozoan material but have not been tested for blood-dwelling stages of nematodes. In this study Whatman FTA Elute (Whatman plc, ...
Eshbaugh Jonathan P - - 2009
This study investigated the effect of high flow conditions on aerosol penetration and the relationship between penetration at constant and cyclic flow conditions. National Institute for Occupational Safety and Health (NIOSH)-approved N95 and P100 filtering facepiece respirators and cartridges were challenged with inert solid and oil aerosols. A combination of ...
Fenton Erin M - - 2009
We have fabricated paper- and nitrocellulose-based lateral-flow devices that are shaped in two dimensions by a computer-controlled knife. The resulting star, candelabra, and other structures are spotted with multiple bioassay reagents to produce multiplex lateral-flow assays. We have also fabricated laminar composites in which porous nitrocellulose media are sandwiched between ...
Gauffin Fredrika - - 2009
BACKGROUND: Diseases with an onset during childhood or adult life can have their origin during fetal life or at birth. Neonatal blood dried on filter paper (Guthrie cards) collected for screening purposes is routinely stored for decades. In addition to clinical use, these filters in combination with patient registers constitute ...
Garritsen Henk S P - - 2009
Serology, defined as antibody-based diagnostics, has been regarded as the diagnostic gold standard in transfusion medicine. Nowadays however the impact of molecular diagnostics in transfusion medicine is rapidly growing. Molecular diagnostics can improve tissue typing (HLA typing), increase safety of blood products (NAT testing of infectious diseases), and enable blood ...
Chang Shih-Hsiang - - 2009
A theoretical study on the transient electro-osmotic flow through a cylindrical microcapillary containing a salt-free medium is presented for both constant surface charge density and constant surface potential. The exact analytical solutions for the electric potential distribution and the transient electro-osmotic flow velocity are derived by solving the nonlinear Poisson-Boltzmann ...
< 1 2 3 4 5 6 7 8 9 10 >